Any feedback?
Literature summary for 1.10.3.2 extracted from
- Atypical laccase isoenzymes from copper supplemented Pleurotus ostreatus cultures (2003), Enzyme Microb. Technol., 33, 220-230.
No PubMed abstract available
General Stability
| General Stability | Organism |
|---|---|
| POXA3a dissociates in 3 M urea, while POXA3b is not dissociated even in 6 M urea | Pleurotus ostreatus |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.036 | - |
syringaldazine | 25°C, isozyme POXA3a | Pleurotus ostreatus |  |
| 0.07 | - |
2,2'-azinobis(3-ethylbenzothiazoline-6-sulfonic acid) | 25°C, isozyme POXA3a | Pleurotus ostreatus | |
| 0.074 | - |
2,2'-azinobis(3-ethylbenzothiazoline-6-sulfonic acid) | 25°C, isozyme POXA3b | Pleurotus ostreatus | |
| 0.079 | - |
syringaldazine | 25°C, isozyme POXA3b | Pleurotus ostreatus | |
| 8.8 | - |
2,6-dimethoxyphenol | 25°C, isozyme POXA3b | Pleurotus ostreatus | |
| 14 | - |
2,6-dimethoxyphenol | 25°C, isozyme POXA3a | Pleurotus ostreatus | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| copper | 3.3 mol copper per mol of protein, type I copper | Pleurotus ostreatus | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 67000 | - |
x * 67000 + x * 16000-18000, SDS-PAGE and MALDI-TOF, both POXA3a and POXA3b | Pleurotus ostreatus |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Pleurotus ostreatus | - |
copper supplemented cultures, isozyme POXA3a activity increases during fungal growth | - |
Posttranslational Modification
| Posttranslational Modification | Comment | Organism |
|---|---|---|
| glycoprotein | carbohydrate content about 10%, two N-glycosilation sites, both POXA3a and POXA3b | Pleurotus ostreatus |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| both POXA3a and POXA3b | Pleurotus ostreatus |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 1050 | - |
25°C, isozyme POXA3b | Pleurotus ostreatus |
| 2000 | - |
25°C, isozyme POXA3a | Pleurotus ostreatus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 2,2'-azinobis(3-ethylbenzothiazoline-6-sulfonic acid) + O2 | - |
Pleurotus ostreatus | ? + H2O | - |
? | |
| 2,6-dimethoxyphenol + O2 | - |
Pleurotus ostreatus | ? + H2O | - |
? | |
| additional information | no significant enzymic activity with guaiacol | Pleurotus ostreatus | ? | - |
? | |
| syringaldazine + O2 | - |
Pleurotus ostreatus | ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 67000 + x * 16000-18000, SDS-PAGE and MALDI-TOF, both POXA3a and POXA3b | Pleurotus ostreatus |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| POXA3a | - |
Pleurotus ostreatus |
| POXA3b | - |
Pleurotus ostreatus |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 35 | - |
POXA3a and POXA3b | Pleurotus ostreatus |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 40 | - |
POXA3a, 50% residual activity after 6 h, POXA3b, 50% residual activity after 14h | Pleurotus ostreatus |
| 60 | - |
POXA3a and POXA3b, 50% residual activity after a few min | Pleurotus ostreatus |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 2830 | - |
syringaldazine | 25°C, isozyme POXA3a | Pleurotus ostreatus | |
| 11670 | - |
syringaldazine | 25°C, isozyme POXA3b | Pleurotus ostreatus | |
| 20000 | - |
2,6-dimethoxyphenol | 25°C, isozyme POXA3b | Pleurotus ostreatus | |
| 23800 | - |
2,6-dimethoxyphenol | 25°C, isozyme POXA3a | Pleurotus ostreatus | |
| 73300 | - |
2,2'-azinobis(3-ethylbenzothiazoline-6-sulfonic acid) | 25°C, isozyme POXA3a | Pleurotus ostreatus | |
| 158300 | - |
2,2'-azinobis(3-ethylbenzothiazoline-6-sulfonic acid) | 25°C, isozyme POXA3b | Pleurotus ostreatus | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 3.6 | - |
POXA3a and POXA3b, substrate 2,2-azinobis(3-ethylbenzothiazoline-6-sulfonic acid) | Pleurotus ostreatus |
| 5.5 | - |
POXA3a and POXA3b, substrate 2,6-dimethoxyphenol | Pleurotus ostreatus |
| 6.2 | - |
POXA3a and POXA3b, substrate syringaldazine | Pleurotus ostreatus |
pH Stability
| pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|
| 5 | 7 | POXA3a and POXA3b, at pH 7.0 after 7 days 50% residual activity | Pleurotus ostreatus |
Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.
Release 2023.1 (January 2023)
Legal
Curated at
Member of
