Inhibitors | Comment | Organism | Structure |
---|---|---|---|
cyanide | 0.1 mM, strong | Trametes sp. | |
Sodium azide | 0.1 mM, strong | Trametes sp. |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.025 | - |
2,2'-azinobis(3-ethylbenzothiazoline-6-sulfonic acid) | pH 4.5, 25°C | Trametes sp. | |
0.0255 | - |
2,6-dimethoxyphenol | pH 4.5, 25°C | Trametes sp. | |
0.42 | - |
guaiacol | pH 4.5, 25°C | Trametes sp. |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
57000 | - |
gel filtration | Trametes sp. |
58500 | - |
1 * 62000, SDS-PAGE, 1 * 58500, MALDI mass spectrometry | Trametes sp. |
62000 | - |
1 * 62000, SDS-PAGE, 1 * 58500, MALDI mass spectrometry | Trametes sp. |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Trametes sp. | Q5I7J0 | two isozymes after induction by kraft lignin, laccase as forms 85% of total activity | - |
Trametes sp. AH28-2 | Q5I7J0 | two isozymes after induction by kraft lignin, laccase as forms 85% of total activity | - |
Posttranslational Modification | Comment | Organism |
---|---|---|
glycoprotein | carbohydrate content 11-12% | Trametes sp. |
Purification (Comment) | Organism |
---|---|
isozyme laccase | Trametes sp. |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
culture supernatant | - |
Trametes sp. | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
66.8 | - |
pH 4.5, 25°C | Trametes sp. |
79 | - |
pH 4.5, 25°C | Trametes sp. |
670 | - |
pH 4.5, 25°C | Trametes sp. |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2,2'-azinobis(3-ethylbenzothiazoline-6-sulfonic acid) + O2 | - |
Trametes sp. | ? + H2O | - |
? | |
2,2'-azinobis(3-ethylbenzothiazoline-6-sulfonic acid) + O2 | - |
Trametes sp. AH28-2 | ? + H2O | - |
? | |
2,6-dichlorophenol + O2 | - |
Trametes sp. | ? + H2O | - |
? | |
2,6-dichlorophenol + O2 | - |
Trametes sp. AH28-2 | ? + H2O | - |
? | |
2,6-dimethoxyphenol + O2 | - |
Trametes sp. | ? + H2O | - |
? | |
2,6-dimethoxyphenol + O2 | - |
Trametes sp. AH28-2 | ? + H2O | - |
? | |
fluorene + O2 | - |
Trametes sp. | ? + H2O | - |
? | |
fluorene + O2 | - |
Trametes sp. AH28-2 | ? + H2O | - |
? | |
guaiacol + O2 | - |
Trametes sp. | ? + H2O | - |
? | |
guaiacol + O2 | - |
Trametes sp. AH28-2 | ? + H2O | - |
? |
Subunits | Comment | Organism |
---|---|---|
monomer | 1 * 62000, SDS-PAGE, 1 * 58500, MALDI mass spectrometry | Trametes sp. |
Synonyms | Comment | Organism |
---|---|---|
laccase A | - |
Trametes sp. |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
50 | - |
substrate guaiacol | Trametes sp. |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
75 | - |
27 min, 50% residual activity | Trametes sp. |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
69 | - |
guaiacol | pH 4.5, 25°C | Trametes sp. | |
81 | - |
2,6-dimethoxyphenol | pH 4.5, 25°C | Trametes sp. | |
692 | - |
2,2'-azinobis(3-ethylbenzothiazoline-6-sulfonic acid) | pH 4.5, 25°C | Trametes sp. |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
4.5 | - |
substrate guaiacol | Trametes sp. |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
4.2 | 8 | good stability | Trametes sp. |
Organism | Comment | pI Value Maximum | pI Value |
---|---|---|---|
Trametes sp. | isoelectric focusing | - |
4.2 |