Inhibitors | Comment | Organism | Structure |
---|---|---|---|
catechol | catechol produces substrate inhibition above 16 mM | Panulirus argus | |
dopamine | dopamine produces substrate inhibition above 2 mM | Panulirus argus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.181 | - |
dopamine | SDS-activated hemocyanin | Panulirus argus | |
2.565 | - |
L-Dopa | SDS-activated hemocyanin | Panulirus argus | |
7.174 | - |
catechol | SDS-activated hemocyanin | Panulirus argus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
77000 | - |
1 * 77000 + 1 * 80000 + 1 * 89000 | Panulirus argus |
80000 | - |
1 * 77000 + 1 * 80000 + 1 * 89000 | Panulirus argus |
89000 | - |
1 * 77000 + 1 * 80000 + 1 * 89000 | Panulirus argus |
170000 | - |
incubation of native hemocyanin with trypsin, produces a band of 170000 Da with intense phenoloxidase activity | Panulirus argus |
190000 | - |
incubation of native hemocyanin with chymotrypsin, produces a band of 190000 Da with intense phenoloxidase activity | Panulirus argus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Panulirus argus | - |
spiny lobster | - |
Purification (Comment) | Organism |
---|---|
Sephacryl S-300 gel filtration | Panulirus argus |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
hemolymph | - |
Panulirus argus | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2 catechol + O2 | - |
Panulirus argus | 2 1,2-benzoquinone + 2 H2O | - |
? | |
2 dopamine + O2 | dopamine is the most specific substrate | Panulirus argus | 2 4-(2-aminoethyl)-1,2-benzoquinone + 2 H2O | - |
? | |
L-Dopa + O2 | - |
Panulirus argus | L-dopaquinone + H2O | - |
? | |
additional information | phenoloxidase activity is not observed when tyramine and tyrosine (monophenols) were used as substrates | Panulirus argus | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
trimer | 1 * 77000 + 1 * 80000 + 1 * 89000 | Panulirus argus |
Synonyms | Comment | Organism |
---|---|---|
hemocyanin | behaves as a catecholoxidase and shows phenoloxidase activity in vitro | Panulirus argus |
phenoloxidase | - |
Panulirus argus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
5.5 | - |
there are two peaks of optimal pH, at pH 5.5 and pH 7.0 | Panulirus argus |
7 | - |
there are two peaks of optimal pH, at pH 5.5 and pH 7.0 | Panulirus argus |