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Literature summary for 1.10.3.1 extracted from

  • Erat, M.; Sakiroglu, H.; Kufrevioglu, O.I.
    Purification and characterization of polyphenol oxidase from Ferula sp. (2006), Food Chem., 95, 503-508.
    View publication on PubMed
Search for more literature for 1.10.3.1

Inhibitors

Inhibitors Comment Organism Structure
2-mercaptoethanol competitive Ferula sp.
L-ascorbic acid noncompetitive Ferula sp.
L-cysteine chloride competitive Ferula sp.
Sodium diethyl dithiocarbamate competitive Ferula sp.
Sodium metabisulfite competitive Ferula sp.

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.31
-
 (+)-catechin pH 6.5, 30°C, stem enzyme Ferula sp.
0.554
-
 (+)-catechin pH 6.5, 30°C, leaf enzyme Ferula sp.
0.764
-
 chlorogenic acid pH 6.0, 20°C, leaf enzyme Ferula sp.
0.798
-
 (-)-epicatechin pH 6.0, 15°C, leaf enzyme Ferula sp.
1.07
-
 chlorogenic acid pH 6.0, 20°C, stem enzyme Ferula sp.
2.34
-
 catechol pH 7.0, 12°C, leaf enzyme Ferula sp.
2.64
-
 catechol pH 7.0, 12°C, stem enzyme Ferula sp.
2.89
-
 (-)-epicatechin pH 6.0, 15°C, stem enzyme Ferula sp.
6.58
-
 4-methylcatechol pH 6.0, 25°C, leaf enzyme Ferula sp.
6.78
-
 4-methylcatechol pH 6.0, 25°C, stem enzyme Ferula sp.

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
(+)-catechin + O2 Ferula sp.
-
 ?
-
 ?
(-)-epicatechin + O2 Ferula sp.
-
 ?
-
 ?
4-methylcatechol + O2 Ferula sp.
-
 4-methyl-1,2-benzoquinone + H2O
-
 ?
catechol + 1/2 O2 Ferula sp.
-
 1,2-benzoquinone + H2O
-
 ?
chlorogenic acid + O2 Ferula sp.
-
 ?
-
 ?
additional information Ferula sp. polyphenol oxidase is a major enzyme responsible for the browning reaction in damaged plant tissues and fruits ?
-
 ?

Organism

Organism UniProt Comment Textmining
Ferula sp.
-
-
-

Purification (Commentary)

Purification (Comment) Organism
native enzyme, 25.8fold from stem and 43.3 from leaves, by (NH4)2SO4 precipitation, dialysis, and gel filtration Ferula sp.

Source Tissue

Source Tissue Comment Organism Textmining
leaf
-
 Ferula sp.
-
root
-
 Ferula sp.
-
stem
-
 Ferula sp.
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
 substrate specificity, tissue specific activities, overview Ferula sp.
13.15
-
 purified stem enzyme Ferula sp.
41.6
-
 purified leaf enzyme Ferula sp.

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(+)-catechin + O2
-
 Ferula sp. ?
-
 ?
(-)-epicatechin + O2
-
 Ferula sp. ?
-
 ?
(-)-epicatechin + O2 high activity Ferula sp. ?
-
 ?
4-methylcatechol + O2
-
 Ferula sp. 4-methyl-1,2-benzoquinone + H2O
-
 ?
catechin + O2
-
 Ferula sp. ?
-
 ?
catechol + 1/2 O2
-
 Ferula sp. 1,2-benzoquinone + H2O
-
 ?
catechol + 1/2 O2 best substrate Ferula sp. 1,2-benzoquinone + H2O
-
 ?
chlorogenic acid + O2
-
 Ferula sp. ?
-
 ?
dopamine + O2 low activity Ferula sp. ?
-
 ?
gallic acid + O2 low activity Ferula sp. ?
-
 ?
L-DOPA + O2 low activity Ferula sp. ?
-
 ?
additional information polyphenol oxidase is a major enzyme responsible for the browning reaction in damaged plant tissues and fruits Ferula sp. ?
-
 ?
additional information substrate specificity, overview, no activity with L-tyrosine Ferula sp. ?
-
 ?
pyrogallol + O2 low activity Ferula sp. ?
-
 ?

Synonyms

Synonyms Comment Organism
polyphenol oxidase
-
 Ferula sp.
PPO
-
 Ferula sp.

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
12 30 dependent on the substrate, overview Ferula sp.

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
5 70
-
 Ferula sp.

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
30
-
 60 min, purified enzyme, 40% reduced activity Ferula sp.
40
-
 60 min, purified enzyme, 65% reduced activity Ferula sp.
50
-
 60 min, purified enzyme, complete inactivation Ferula sp.
60
-
 40 min, purified enzyme, irreversible denaturation Ferula sp.

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6 7 dependent on the substrate, overview Ferula sp.

pH Range

pH Minimum pH Maximum Comment Organism
5 7.5
-
 Ferula sp.

pH Stability

pH Stability pH Stability Maximum Comment Organism
5 7.5
-
 Ferula sp.

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
0.0259
-
 Sodium metabisulfite pH 7.0, stem enzyme Ferula sp.
0.0454
-
 Sodium diethyl dithiocarbamate pH 7.0, stem enzyme Ferula sp.
0.0632
-
 2-mercaptoethanol pH 7.0, stem enzyme Ferula sp.
0.0645
-
 Sodium diethyl dithiocarbamate pH 7.0, leaf enzyme Ferula sp.
0.0718
-
 L-ascorbic acid pH 7.0, stem enzyme Ferula sp.
0.0815
-
 L-ascorbic acid pH 7.0, leaf enzyme Ferula sp.
0.116
-
 L-cysteine chloride pH 7.0, stem enzyme Ferula sp.
0.119
-
 Sodium metabisulfite pH 7.0, leaf enzyme Ferula sp.
0.126
-
 L-cysteine chloride pH 7.0, leaf enzyme Ferula sp.
0.138
-
 2-mercaptoethanol pH 7.0, leaf enzyme Ferula sp.