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Literature summary for 1.10.3.1 extracted from

  • Mazzafera, P.; Robinson, S.P.
    Characterization of polyphenol oxidase in coffee (2000), Phytochemistry, 55, 285-296.
    View publication on PubMed

Inhibitors

Inhibitors Comment Organism Structure
Cinnamic acid
-
Coffea arabica
hexadecyltrimethyl-ammonium bromide
-
Coffea arabica
polyvinylpyrrolidone 40
-
Coffea arabica
Salicylhydroxamic acid
-
Coffea arabica

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.88
-
chlorogenic acid leaf enzyme Coffea arabica
2.27
-
chlorogenic acid endosperm enzyme Coffea arabica

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
chlorogenic acid + O2 Coffea arabica probably the major substrate in vivo ?
-
?

Organism

Organism UniProt Comment Textmining
Coffea arabica
-
-
-

Source Tissue

Source Tissue Comment Organism Textmining
fruit endosperm Coffea arabica
-
leaf
-
Coffea arabica
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4-methylcatechol + O2
-
Coffea arabica 4-methyl-1,2-benzoquinone
-
?
chlorogenic acid + O2 best substrate for both leaf and endosperm enzyme Coffea arabica ?
-
?
chlorogenic acid + O2 probably the major substrate in vivo Coffea arabica ?
-
?
L-dopa + 1/2 O2 endosperm enzyme, 3.9% of activity with chlorogenic acid Coffea arabica L-dopaquinone + H2O
-
?

Subunits

Subunits Comment Organism
More enzyme may be synthesized as a 67000 Da precursor that can be hydrolyzed to a 45000 Da form still retaining catalytic activity Coffea arabica

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
25 30 leaf and endosperm enzyme Coffea arabica

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
50
-
leaf enzyme, no loss of activity after 30 min, 50% loss of activity after 5 min at 80°C, endosperm enzyme, 20% loss of activity after 30 min at 50°C, 50% loss of activity after 2.5 min at 80°C Coffea arabica

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6 7 leaf and endosperm enzyme Coffea arabica