Cloned (Comment) | Organism |
---|---|
expressed in Pichia pastoris strain KM-71H | Pseudomonas putida |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.21 | - |
ethanol | at pH 9.0 and 25°C | Pseudomonas putida | |
0.43 | - |
1-propanol | at pH 9.0 and 25°C | Pseudomonas putida | |
2.4 | - |
propionaldehyde | at pH 9.0 and 25°C | Pseudomonas putida | |
5.8 | - |
acetaldehyde | at pH 9.0 and 25°C | Pseudomonas putida |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Ca2+ | required | Pseudomonas putida |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Pseudomonas putida | - |
- |
- |
Pseudomonas putida KT 2240 | - |
- |
- |
Purification (Comment) | Organism |
---|---|
ammonium sulfate precipitation, phenyl-Toyopearl 650S column chromatography, and DEAE-Toyopearl 650S column chromatography | Pseudomonas putida |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
1,2-propanediol + 2,6-dichlorophenolindophenol | 9.1% activity compared to 1-propanol | Pseudomonas putida | ? + reduced 2,6-dichlorophenolindophenol | - |
r | |
1,3-propanediol + 2,6-dichlorophenolindophenol | 12.2% activity compared to 1-propanol | Pseudomonas putida | ? + reduced 2,6-dichlorophenolindophenol | - |
r | |
1,4-butanediol + 2,6-dichlorophenolindophenol | 16.2% activity compared to 1-propanol | Pseudomonas putida | ? + reduced 2,6-dichlorophenolindophenol | - |
r | |
1-butanol + 2,6-dichlorophenolindophenol | 82% activity compared to 1-propanol | Pseudomonas putida | butyraldehyde + reduced 2,6-dichlorophenolindophenol | - |
r | |
1-butanol + 2,6-dichlorophenolindophenol | 82% activity compared to 1-propanol | Pseudomonas putida KT 2240 | butyraldehyde + reduced 2,6-dichlorophenolindophenol | - |
r | |
1-heptanol + 2,6-dichlorophenolindophenol | 44.1% activity compared to 1-propanol | Pseudomonas putida | heptanal + reduced 2,6-dichlorophenolindophenol | - |
r | |
1-heptanol + 2,6-dichlorophenolindophenol | 44.1% activity compared to 1-propanol | Pseudomonas putida KT 2240 | heptanal + reduced 2,6-dichlorophenolindophenol | - |
r | |
1-hexanol + 2,6-dichlorophenolindophenol | 62.2% activity compared to 1-propanol | Pseudomonas putida | hexanal + reduced 2,6-dichlorophenolindophenol | - |
r | |
1-hexanol + 2,6-dichlorophenolindophenol | 62.2% activity compared to 1-propanol | Pseudomonas putida KT 2240 | hexanal + reduced 2,6-dichlorophenolindophenol | - |
r | |
1-pentanol + 2,6-dichlorophenolindophenol | 85.5% activity compared to 1-propanol | Pseudomonas putida | pentanaldehyde + reduced 2,6-dichlorophenolindophenol | - |
r | |
1-propanol + 2,6-dichlorophenolindophenol | - |
Pseudomonas putida | propionaldehyde + reduced 2,6-dichlorophenolindophenol | - |
r | |
1-propanol + phenazine methosulfate | 100% activity | Pseudomonas putida | propionaldehyde + reduced phenazine methosulfate | - |
r | |
2,3-butanediol + 2,6-dichlorophenolindophenol | 4.1% activity compared to 1-propanol | Pseudomonas putida | ? + reduced 2,6-dichlorophenolindophenol | - |
r | |
2-(S)-butanol + 2,6-dichlorophenolindophenol | 43.3% activity compared to 1-propanol | Pseudomonas putida | ? + reduced 2,6-dichlorophenolindophenol | - |
r | |
2-propanol + 2,6-dichlorophenolindophenol | 31.9% activity compared to 1-propanol | Pseudomonas putida | ? + reduced 2,6-dichlorophenolindophenol | - |
r | |
acetaldehyde + reduced 2,6-dichlorophenolindophenol | - |
Pseudomonas putida | ethanol + 2,6-dichlorophenolindophenol | - |
r | |
acetaldehyde + reduced phenazine methosulfate | 55.6% activity compared to 1-propanol | Pseudomonas putida | ethanol + phenazine methosulfate | - |
r | |
benzyl alcohol + 2,6-dichlorophenolindophenol | 3.9% activity compared to 1-propanol | Pseudomonas putida | benzaldehyde + reduced 2,6-dichlorophenolindophenol | - |
r | |
benzyl alcohol + 2,6-dichlorophenolindophenol | 3.9% activity compared to 1-propanol | Pseudomonas putida KT 2240 | benzaldehyde + reduced 2,6-dichlorophenolindophenol | - |
r | |
butyraldehyde + reduced 2,6-dichlorophenolindophenol | 32.1% activity compared to 1-propanol | Pseudomonas putida | 1-butanol + 2,6-dichlorophenolindophenol | - |
r | |
cyclohexanol + 2,6-dichlorophenolindophenol | 5.5% activity compared to 1-propanol | Pseudomonas putida | ? + reduced 2,6-dichlorophenolindophenol | - |
r | |
ethanol + 2,6-dichlorophenolindophenol | - |
Pseudomonas putida | acetaldehyde + reduced 2,6-dichlorophenolindophenol | - |
r | |
ethanol + 2,6-dichlorophenolindophenol | - |
Pseudomonas putida KT 2240 | acetaldehyde + reduced 2,6-dichlorophenolindophenol | - |
r | |
ethanol + phenazine methosulfate | 88.9% activity compared to 1-propanol | Pseudomonas putida | acetaldehyde + reduced phenazine methosulfate | - |
r | |
methanol + phenazine methosulfate | 6.3% activity compared to 1-propanol | Pseudomonas putida | formaldehyde + reduced phenazine methosulfate | - |
r | |
propionaldehyde + reduced 2,6-dichlorophenolindophenol | - |
Pseudomonas putida | 1-propanol + 2,6-dichlorophenolindophenol | - |
r | |
propionaldehyde + reduced phenazine methosulfate | 54.5% activity compared to 1-propanol | Pseudomonas putida | 1-propanol + phenazine methosulfate | - |
r |
Synonyms | Comment | Organism |
---|---|---|
PQQ-ADH | - |
Pseudomonas putida |
pyrroloquinoline quinone dependent alcohol dehydrogenase | - |
Pseudomonas putida |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
22 | - |
propionaldehyde | at pH 9.0 and 25°C | Pseudomonas putida | |
24.8 | - |
ethanol | at pH 9.0 and 25°C | Pseudomonas putida | |
31.1 | - |
acetaldehyde | at pH 9.0 and 25°C | Pseudomonas putida | |
32.6 | - |
1-propanol | at pH 9.0 and 25°C | Pseudomonas putida |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
9 | - |
- |
Pseudomonas putida |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
5.4 | - |
acetaldehyde | at pH 9.0 and 25°C | Pseudomonas putida | |
9.2 | - |
propionaldehyde | at pH 9.0 and 25°C | Pseudomonas putida | |
75.8 | - |
1-propanol | at pH 9.0 and 25°C | Pseudomonas putida | |
118.1 | - |
ethanol | at pH 9.0 and 25°C | Pseudomonas putida |