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Literature summary for 1.1.5.5 extracted from

  • Takeda, K.; Matsumura, H.; Ishida, T.; Samejima, M.; Igarashi, K.; Nakamura, N.; Ohno, H.
    The two-step electrochemical oxidation of alcohols using a novel recombinant PQQ alcohol dehydrogenase as a catalyst for a bioanode (2013), Bioelectrochemistry, 94, 75-78.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Pichia pastoris strain KM-71H Pseudomonas putida

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.21
-
ethanol at pH 9.0 and 25°C Pseudomonas putida
0.43
-
1-propanol at pH 9.0 and 25°C Pseudomonas putida
2.4
-
propionaldehyde at pH 9.0 and 25°C Pseudomonas putida
5.8
-
acetaldehyde at pH 9.0 and 25°C Pseudomonas putida

Metals/Ions

Metals/Ions Comment Organism Structure
Ca2+ required Pseudomonas putida

Organism

Organism UniProt Comment Textmining
Pseudomonas putida
-
-
-
Pseudomonas putida KT 2240
-
-
-

Purification (Commentary)

Purification (Comment) Organism
ammonium sulfate precipitation, phenyl-Toyopearl 650S column chromatography, and DEAE-Toyopearl 650S column chromatography Pseudomonas putida

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1,2-propanediol + 2,6-dichlorophenolindophenol 9.1% activity compared to 1-propanol Pseudomonas putida ? + reduced 2,6-dichlorophenolindophenol
-
r
1,3-propanediol + 2,6-dichlorophenolindophenol 12.2% activity compared to 1-propanol Pseudomonas putida ? + reduced 2,6-dichlorophenolindophenol
-
r
1,4-butanediol + 2,6-dichlorophenolindophenol 16.2% activity compared to 1-propanol Pseudomonas putida ? + reduced 2,6-dichlorophenolindophenol
-
r
1-butanol + 2,6-dichlorophenolindophenol 82% activity compared to 1-propanol Pseudomonas putida butyraldehyde + reduced 2,6-dichlorophenolindophenol
-
r
1-butanol + 2,6-dichlorophenolindophenol 82% activity compared to 1-propanol Pseudomonas putida KT 2240 butyraldehyde + reduced 2,6-dichlorophenolindophenol
-
r
1-heptanol + 2,6-dichlorophenolindophenol 44.1% activity compared to 1-propanol Pseudomonas putida heptanal + reduced 2,6-dichlorophenolindophenol
-
r
1-heptanol + 2,6-dichlorophenolindophenol 44.1% activity compared to 1-propanol Pseudomonas putida KT 2240 heptanal + reduced 2,6-dichlorophenolindophenol
-
r
1-hexanol + 2,6-dichlorophenolindophenol 62.2% activity compared to 1-propanol Pseudomonas putida hexanal + reduced 2,6-dichlorophenolindophenol
-
r
1-hexanol + 2,6-dichlorophenolindophenol 62.2% activity compared to 1-propanol Pseudomonas putida KT 2240 hexanal + reduced 2,6-dichlorophenolindophenol
-
r
1-pentanol + 2,6-dichlorophenolindophenol 85.5% activity compared to 1-propanol Pseudomonas putida pentanaldehyde + reduced 2,6-dichlorophenolindophenol
-
r
1-propanol + 2,6-dichlorophenolindophenol
-
Pseudomonas putida propionaldehyde + reduced 2,6-dichlorophenolindophenol
-
r
1-propanol + phenazine methosulfate 100% activity Pseudomonas putida propionaldehyde + reduced phenazine methosulfate
-
r
2,3-butanediol + 2,6-dichlorophenolindophenol 4.1% activity compared to 1-propanol Pseudomonas putida ? + reduced 2,6-dichlorophenolindophenol
-
r
2-(S)-butanol + 2,6-dichlorophenolindophenol 43.3% activity compared to 1-propanol Pseudomonas putida ? + reduced 2,6-dichlorophenolindophenol
-
r
2-propanol + 2,6-dichlorophenolindophenol 31.9% activity compared to 1-propanol Pseudomonas putida ? + reduced 2,6-dichlorophenolindophenol
-
r
acetaldehyde + reduced 2,6-dichlorophenolindophenol
-
Pseudomonas putida ethanol + 2,6-dichlorophenolindophenol
-
r
acetaldehyde + reduced phenazine methosulfate 55.6% activity compared to 1-propanol Pseudomonas putida ethanol + phenazine methosulfate
-
r
benzyl alcohol + 2,6-dichlorophenolindophenol 3.9% activity compared to 1-propanol Pseudomonas putida benzaldehyde + reduced 2,6-dichlorophenolindophenol
-
r
benzyl alcohol + 2,6-dichlorophenolindophenol 3.9% activity compared to 1-propanol Pseudomonas putida KT 2240 benzaldehyde + reduced 2,6-dichlorophenolindophenol
-
r
butyraldehyde + reduced 2,6-dichlorophenolindophenol 32.1% activity compared to 1-propanol Pseudomonas putida 1-butanol + 2,6-dichlorophenolindophenol
-
r
cyclohexanol + 2,6-dichlorophenolindophenol 5.5% activity compared to 1-propanol Pseudomonas putida ? + reduced 2,6-dichlorophenolindophenol
-
r
ethanol + 2,6-dichlorophenolindophenol
-
Pseudomonas putida acetaldehyde + reduced 2,6-dichlorophenolindophenol
-
r
ethanol + 2,6-dichlorophenolindophenol
-
Pseudomonas putida KT 2240 acetaldehyde + reduced 2,6-dichlorophenolindophenol
-
r
ethanol + phenazine methosulfate 88.9% activity compared to 1-propanol Pseudomonas putida acetaldehyde + reduced phenazine methosulfate
-
r
methanol + phenazine methosulfate 6.3% activity compared to 1-propanol Pseudomonas putida formaldehyde + reduced phenazine methosulfate
-
r
propionaldehyde + reduced 2,6-dichlorophenolindophenol
-
Pseudomonas putida 1-propanol + 2,6-dichlorophenolindophenol
-
r
propionaldehyde + reduced phenazine methosulfate 54.5% activity compared to 1-propanol Pseudomonas putida 1-propanol + phenazine methosulfate
-
r

Synonyms

Synonyms Comment Organism
PQQ-ADH
-
Pseudomonas putida
pyrroloquinoline quinone dependent alcohol dehydrogenase
-
Pseudomonas putida

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
22
-
propionaldehyde at pH 9.0 and 25°C Pseudomonas putida
24.8
-
ethanol at pH 9.0 and 25°C Pseudomonas putida
31.1
-
acetaldehyde at pH 9.0 and 25°C Pseudomonas putida
32.6
-
1-propanol at pH 9.0 and 25°C Pseudomonas putida

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
9
-
-
Pseudomonas putida

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
5.4
-
acetaldehyde at pH 9.0 and 25°C Pseudomonas putida
9.2
-
propionaldehyde at pH 9.0 and 25°C Pseudomonas putida
75.8
-
1-propanol at pH 9.0 and 25°C Pseudomonas putida
118.1
-
ethanol at pH 9.0 and 25°C Pseudomonas putida