Cloned (Comment) | Organism |
---|---|
expression in Pichia pastoris | Colletotrichum graminicola |
Crystallization (Comment) | Organism |
---|---|
homology modeling of structure. The central, seven-bladed beta-propeller domain and the C-terminal, immunoglobulin-like beta-sandwich together constitute the AA5_2 catalytic module (residues Val199 to Leu689). The N-terminal domain contains conserved disulfide bridges and an overall fold typical of a PAN_1 domain | Colletotrichum graminicola |
Protein Variants | Comment | Organism |
---|---|---|
Y334F | mutant exhibits specific activities comparable to the wild-type for carbohydrates, diols, aryl alcohols, 5-hydroxymethylfurfural and 5-hydroxymethyl-2-furancarboxylic acid | Colletotrichum graminicola |
Y334W | mutant exhibits increased specific activity toward carbohydrates and decreased activity toward diols, aryl alcohols, and furans | Colletotrichum graminicola |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
4.5 | - |
4-Hydroxybenzyl alcohol | wild-type, pH 7, 25°C | Colletotrichum graminicola | |
6.5 | - |
5-hydroxymethylfurfural | wild-type, pH 7, 25°C | Colletotrichum graminicola | |
14 | - |
5-hydroxymethylfurfural | mutant Y334F, pH 7, 25°C | Colletotrichum graminicola | |
19 | - |
4-Hydroxybenzyl alcohol | mutant Y334F, pH 7, 25°C | Colletotrichum graminicola | |
21 | - |
3-anisyl alcohol | wild-type, pH 7, 25°C | Colletotrichum graminicola | |
24 | - |
4-anisyl alcohol | wild-type, pH 7, 25°C | Colletotrichum graminicola | |
26.9 | - |
5-(hydroxymethyl)furan-2-carboxylic acid | wild-type, pH 7, 25°C | Colletotrichum graminicola | |
27 | - |
benzyl alcohol | wild-type, pH 7, 25°C | Colletotrichum graminicola | |
27 | - |
5-(hydroxymethyl)furan-2-carboxylic acid | mutant Y334F, pH 7, 25°C | Colletotrichum graminicola | |
42 | - |
5-(hydroxymethyl)furan-2-carboxylic acid | mutant Y334W, pH 7, 25°C | Colletotrichum graminicola | |
52.3 | - |
3-anisyl alcohol | mutant Y334F, pH 7, 25°C | Colletotrichum graminicola | |
53 | - |
5-hydroxymethylfurfural | mutant Y334W, pH 7, 25°C | Colletotrichum graminicola | |
61 | - |
benzyl alcohol | mutant Y334F, pH 7, 25°C | Colletotrichum graminicola | |
88 | - |
4-anisyl alcohol | mutant Y334F, pH 7, 25°C | Colletotrichum graminicola |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
copper | enzyme shows a copper-to-protein ratio of 1.08 | Colletotrichum graminicola |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Colletotrichum graminicola | E3Q9X3 | cf. EC 1.1.3.47 | - |
Colletotrichum graminicola M1.001 | E3Q9X3 | cf. EC 1.1.3.47 | - |
Posttranslational Modification | Comment | Organism |
---|---|---|
glycoprotein | protein is predicted to be N-glycosylated on sites N309, N386, and N644 | Colletotrichum graminicola |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
3-anisyl alcohol + O2 | - |
Colletotrichum graminicola | 3-anisaldehyde + H2O2 | - |
? | |
4-anisyl alcohol + O2 | - |
Colletotrichum graminicola | 4-anisaldehyde + H2O2 | - |
? | |
4-anisyl alcohol + O2 | - |
Colletotrichum graminicola M1.001 | 4-anisaldehyde + H2O2 | - |
? | |
4-hydroxybenzyl alcohol + O2 | - |
Colletotrichum graminicola | 4-hydroxybenzaldehyde + H2O2 | - |
? | |
4-hydroxybenzyl alcohol + O2 | - |
Colletotrichum graminicola M1.001 | 4-hydroxybenzaldehyde + H2O2 | - |
? | |
5-(hydroxymethyl)furan-2-carboxylic acid + O2 | - |
Colletotrichum graminicola | 2,5-formylfurancarboxylic acid + H2O2 | - |
? | |
5-hydroxymethylfurfural + O2 | - |
Colletotrichum graminicola | 5-(hydroxymethyl)furan-2-carboxylic acid + H2O2 | - |
? | |
benzyl alcohol + O2 | - |
Colletotrichum graminicola | benzaldehyde + H2O2 | - |
? | |
benzyl alcohol + O2 | - |
Colletotrichum graminicola M1.001 | benzaldehyde + H2O2 | - |
? | |
additional information | enzyme is active on short-chain alkane diols and glycerol, and aryl alcohols, with similar specific activity values. Highest specific activity is observed on 5-hydroxymethylfurfural. Galactose and galactosylated oligosaccharides are poor substrates | Colletotrichum graminicola | ? | - |
- |
|
additional information | enzyme is active on short-chain alkane diols and glycerol, and aryl alcohols, with similar specific activity values. Highest specific activity is observed on 5-hydroxymethylfurfural. Galactose and galactosylated oligosaccharides are poor substrates | Colletotrichum graminicola M1.001 | ? | - |
- |
|
veratryl alcohol + O2 | - |
Colletotrichum graminicola | veratryl aldehyde + H2O2 | - |
? | |
veratryl alcohol + O2 | - |
Colletotrichum graminicola M1.001 | veratryl aldehyde + H2O2 | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 77500, SDS-PAGE, and x * 77507, calculated from sequence, deglycosylated protein | Colletotrichum graminicola |
Synonyms | Comment | Organism |
---|---|---|
GLRG_02805 | - |
Colletotrichum graminicola |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
25 | - |
initial loss of 10% of activity, thereafter stable or at least 22 h | Colletotrichum graminicola |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
3.7 | - |
5-(hydroxymethyl)furan-2-carboxylic acid | mutant Y334W, pH 7, 25°C | Colletotrichum graminicola | |
15.4 | - |
5-(hydroxymethyl)furan-2-carboxylic acid | mutant Y334F, pH 7, 25°C | Colletotrichum graminicola | |
20.1 | - |
4-Hydroxybenzyl alcohol | wild-type, pH 7, 25°C | Colletotrichum graminicola | |
28.3 | - |
5-(hydroxymethyl)furan-2-carboxylic acid | wild-type, pH 7, 25°C | Colletotrichum graminicola | |
33 | - |
4-Hydroxybenzyl alcohol | mutant Y334F, pH 7, 25°C | Colletotrichum graminicola | |
48 | - |
4-anisyl alcohol | wild-type, pH 7, 25°C | Colletotrichum graminicola | |
54.5 | - |
benzyl alcohol | wild-type, pH 7, 25°C | Colletotrichum graminicola | |
71 | - |
5-hydroxymethylfurfural | mutant Y334W, pH 7, 25°C | Colletotrichum graminicola | |
126 | - |
5-hydroxymethylfurfural | wild-type, pH 7, 25°C | Colletotrichum graminicola | |
140 | - |
3-anisyl alcohol | wild-type, pH 7, 25°C | Colletotrichum graminicola | |
201 | - |
5-hydroxymethylfurfural | mutant Y334F, pH 7, 25°C | Colletotrichum graminicola | |
218 | - |
4-anisyl alcohol | mutant Y334F, pH 7, 25°C | Colletotrichum graminicola | |
226 | - |
benzyl alcohol | mutant Y334F, pH 7, 25°C | Colletotrichum graminicola | |
289 | - |
3-anisyl alcohol | mutant Y334F, pH 7, 25°C | Colletotrichum graminicola |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7 | - |
substrate5-hydroxymethylfurfural | Colletotrichum graminicola |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
5.5 | - |
50% of the activity at pH 7.0, substrate 5-hydroxymethylfurfural | Colletotrichum graminicola |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.088 | - |
5-(hydroxymethyl)furan-2-carboxylic acid | mutant Y334W, pH 7, 25°C | Colletotrichum graminicola | |
0.45 | - |
4-Hydroxybenzyl alcohol | mutant Y334W, pH 7, 25°C | Colletotrichum graminicola | |
0.57 | - |
5-(hydroxymethyl)furan-2-carboxylic acid | mutant Y334F, pH 7, 25°C | Colletotrichum graminicola | |
0.66 | - |
4-anisyl alcohol | mutant Y334W, pH 7, 25°C | Colletotrichum graminicola | |
0.75 | - |
benzyl alcohol | mutant Y334W, pH 7, 25°C | Colletotrichum graminicola | |
0.85 | - |
3-anisyl alcohol | mutant Y334W, pH 7, 25°C | Colletotrichum graminicola | |
0.97 | - |
veratryl alcohol | mutant Y334W, pH 7, 25°C | Colletotrichum graminicola | |
1.1 | - |
5-(hydroxymethyl)furan-2-carboxylic acid | wild-type, pH 7, 25°C | Colletotrichum graminicola | |
1.34 | - |
5-hydroxymethylfurfural | mutant Y334W, pH 7, 25°C | Colletotrichum graminicola | |
1.74 | - |
4-Hydroxybenzyl alcohol | mutant Y334F, pH 7, 25°C | Colletotrichum graminicola | |
2 | - |
benzyl alcohol | wild-type, pH 7, 25°C | Colletotrichum graminicola | |
2 | - |
4-anisyl alcohol | wild-type, pH 7, 25°C | Colletotrichum graminicola | |
2.47 | - |
4-anisyl alcohol | mutant Y334F, pH 7, 25°C | Colletotrichum graminicola | |
3.7 | - |
benzyl alcohol | mutant Y334F, pH 7, 25°C | Colletotrichum graminicola | |
4.4 | - |
veratryl alcohol | mutant Y334F, pH 7, 25°C | Colletotrichum graminicola | |
4.5 | - |
4-Hydroxybenzyl alcohol | wild-type, pH 7, 25°C | Colletotrichum graminicola | |
4.7 | - |
veratryl alcohol | wild-type, pH 7, 25°C | Colletotrichum graminicola | |
5.5 | - |
3-anisyl alcohol | mutant Y334F, pH 7, 25°C | Colletotrichum graminicola | |
6.6 | - |
3-anisyl alcohol | wild-type, pH 7, 25°C | Colletotrichum graminicola | |
14.4 | - |
5-hydroxymethylfurfural | mutant Y334F, pH 7, 25°C | Colletotrichum graminicola | |
19.4 | - |
5-hydroxymethylfurfural | wild-type, pH 7, 25°C | Colletotrichum graminicola |