Cloned (Comment) | Organism |
---|---|
gene aao, expression of FLAG1-tagged enzyme in Escherichia coli strain W3110, the non-glycosylated recombinant enzyme is successfully activated in vitro after Escherichia coli expression in form of inclusion bodies, expression iand glycosylation of the enzyme in Emericella nidulans strain IJFM A729 | Pleurotus eryngii |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.028 | - |
4-anisyl alcohol | pH 8.0, 25°C, recombinant enzyme from Emericella nidulans | Pleurotus eryngii | |
0.037 | - |
4-anisyl alcohol | pH 8.0, 25°C, recombinant enzyme from Escherichia coli | Pleurotus eryngii | |
0.04 | - |
4-anisyl alcohol | pH 8.0, 25°C, wild-type enzyme | Pleurotus eryngii | |
0.092 | - |
2,4-hexadien-1-ol | pH 8.0, 25°C, recombinant enzyme from Emericella nidulans | Pleurotus eryngii | |
0.12 | - |
2,4-hexadien-1-ol | pH 8.0, 25°C, recombinant enzyme from Escherichia coli | Pleurotus eryngii | |
0.22 | - |
3-anisyl alcohol | pH 8.0, 25°C, wild-type enzyme | Pleurotus eryngii | |
0.269 | - |
3-anisyl alcohol | pH 8.0, 25°C, recombinant enzyme from Escherichia coli | Pleurotus eryngii | |
0.293 | - |
3-anisyl alcohol | pH 8.0, 25°C, recombinant enzyme from Emericella nidulans | Pleurotus eryngii | |
0.41 | - |
veratryl alcohol | pH 8.0, 25°C, wild-type enzyme | Pleurotus eryngii | |
0.541 | - |
veratryl alcohol | pH 8.0, 25°C, recombinant enzyme from Escherichia coli | Pleurotus eryngii | |
0.59 | 1 | veratryl alcohol | pH 8.0, 25°C, recombinant enzyme from Emericella nidulans | Pleurotus eryngii | |
0.758 | - |
benzyl alcohol | pH 8.0, 25°C, recombinant enzyme from Emericella nidulans | Pleurotus eryngii | |
0.84 | - |
benzyl alcohol | pH 8.0, 25°C, wild-type enzyme | Pleurotus eryngii | |
0.873 | - |
benzyl alcohol | pH 8.0, 25°C, recombinant enzyme from Escherichia coli | Pleurotus eryngii |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
61847 | - |
x * 61847, recombinant enzyme from Escherichia coli, mass spectrometry, x * 69114, wild-type enzyme, mass spectrometry, x * 69792, recombinant enzyme from Emericella nidulans, mass spectrometry | Pleurotus eryngii |
69114 | - |
x * 61847, recombinant enzyme from Escherichia coli, mass spectrometry, x * 69114, wild-type enzyme, mass spectrometry, x * 69792, recombinant enzyme from Emericella nidulans, mass spectrometry | Pleurotus eryngii |
69792 | - |
x * 61847, recombinant enzyme from Escherichia coli, mass spectrometry, x * 69114, wild-type enzyme, mass spectrometry, x * 69792, recombinant enzyme from Emericella nidulans, mass spectrometry | Pleurotus eryngii |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | Pleurotus eryngii | the enzyme provides H2O2 for fungal degradation of lignin | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Pleurotus eryngii | O94219 | precursor; gene aao | - |
Posttranslational Modification | Comment | Organism |
---|---|---|
glycoprotein | - |
Pleurotus eryngii |
Purification (Comment) | Organism |
---|---|
recombinant FLAG1-tagged enzyme in Escherichia coli strain W3110 solubilized from inclusion bodies 9.6fold by anion exchange chromatography | Pleurotus eryngii |
Renatured (Comment) | Organism |
---|---|
the non-glycosylated recombinant enzyme is successfully activated in vitro after Escherichia coli expression in form of inclusion bodies | Pleurotus eryngii |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
96 | - |
purified recombinant enzyme from Escherichia coli | Pleurotus eryngii |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2,4-hexadien-1-ol + O2 | - |
Pleurotus eryngii | 2,4-hexadienal + H2O2 | - |
? | |
3-anisyl alcohol + O2 | - |
Pleurotus eryngii | 3-anisyl aldehyde + H2O2 | - |
? | |
4-anisyl alcohol + O2 | - |
Pleurotus eryngii | 4-anisyl aldehyde + H2O2 | - |
? | |
benzyl alcohol + O2 | - |
Pleurotus eryngii | benzaldehyde + H2O2 | - |
? | |
additional information | the enzyme provides H2O2 for fungal degradation of lignin | Pleurotus eryngii | ? | - |
? | |
additional information | oxidation of aromatic and aliphatic polyunsaturated primary alcohols by wild-type and recombinant enzymes, overview | Pleurotus eryngii | ? | - |
? | |
veratryl alcohol + O2 | - |
Pleurotus eryngii | veratryl aldehyde + H2O2 | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 61847, recombinant enzyme from Escherichia coli, mass spectrometry, x * 69114, wild-type enzyme, mass spectrometry, x * 69792, recombinant enzyme from Emericella nidulans, mass spectrometry | Pleurotus eryngii |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
25 | - |
assay at | Pleurotus eryngii |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8 | - |
assay at | Pleurotus eryngii |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
FAD | a flavoenzyme | Pleurotus eryngii |