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Literature summary for 1.1.3.7 extracted from

  • Bourbonnais, R.; Paice, M.G.
    Veratryl alcohol oxidases from the lignin-degrading basidiomycete Pleurotus sajor-caju (1988), Biochem. J., 255, 445-450.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 1.1.3.7

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.41
-
 3,4-dimethoxybenzyl alcohol enzyme form VAO I Lentinus sajor-caju
0.46
-
 3,4-dimethoxybenzyl alcohol enzyme form VAO II Lentinus sajor-caju

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
71000
-
 x * 71000, SDS-PAGE Lentinus sajor-caju

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
additional information Lentinus sajor-caju production of H2O2 during oxidation of lignin fragments ?
-
 ?

Organism

Organism UniProt Comment Textmining
Lentinus sajor-caju
-
-
-

Posttranslational Modification

Posttranslational Modification Comment Organism
glycoprotein
-
 Lentinus sajor-caju

Purification (Commentary)

Purification (Comment) Organism
2 enzyme forms: VAO I and VAO II Lentinus sajor-caju

Source Tissue

Source Tissue Comment Organism Textmining
culture medium
-
 Lentinus sajor-caju
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
30.7
-
-
 Lentinus sajor-caju

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2,4-dimethoxybenzyl alcohol + O2 50% of the activity with 3,4-dimethoxybenzyl alcohol, VAO I. 75% of the activity with 3,4-dimethoxybenzyl alcohol, VAO II Lentinus sajor-caju 2,4-dimethoxybenzyl aldehyde + H2O2
-
 ?
3,4-dimethoxybenzyl alcohol + O2 i.e. veratryl alcohol Lentinus sajor-caju veratryl aldehyde + H2O2
-
 ?
3,5-dimethoxybenzyl alcohol + O2 7% of the activity with 3,4-dimethoxybenzyl alcohol, VAO I. 8% of the activity with 3,4-dimethoxybenzyl alcohol, VAO II Lentinus sajor-caju 3,5-dimethoxy benzaldehyde + H2O2
-
 ?
3-hydroxy-4-methoxybenzyl alcohol + O2 62% of the activity with 3,4-dimethoxybenzyl alcohol, VAO I. 71% of the activity with 3,4-dimethoxybenzyl alcohol, VAO II Lentinus sajor-caju 3-hydroxy-4-methoxybenzaldehyde + H2O2
-
 ?
3-methoxybenzyl alcohol + O2 19% of the activity with 3,4-dimethoxybenzyl alcohol, VAO I. 16% of the activity with 3,4-dimethoxybenzyl alcohol, VAO II Lentinus sajor-caju 3-methoxybenzaldehyde + H2O2
-
 ?
3-phenoxybenzyl alcohol + O2 35% of the activity with 3,4-dimethoxybenzyl alcohol, VAO I. 18% of the activity with 3,4-dimethoxybenzyl alcohol, VAO II Lentinus sajor-caju 3-phenoxybenzaldehyde + H2O2
-
 ?
4-methoxybenzyl alcohol + O2 200% of the activity with 3,4-dimethoxybenzyl alcohol, VAO I. 266% of the activity with 3,4-dimethoxybenzyl alcohol, VAO II Lentinus sajor-caju 4-methoxybenzaldehyde + H2O2
-
 ?
benzyl alcohol + O2 30% of the activity with 3,4-dimethoxybenzyl alcohol, VAO I. 25% of the activity with 3,4-dimethoxybenzyl alcohol, VAO II Lentinus sajor-caju benzaldehyde + H2O2
-
 ?
cinnamyl alcohol + O2 oxidation at the gamma position, 77% of the activity with 3,4-dimethoxybenzyl alcohol, VAO I. 55% of the activity with 3,4-dimethoxybenzyl alcohol, VAO II Lentinus sajor-caju cinnamic aldehyde + H2O2
-
 ?
additional information production of H2O2 during oxidation of lignin fragments Lentinus sajor-caju ?
-
 ?

Subunits

Subunits Comment Organism
? x * 71000, SDS-PAGE Lentinus sajor-caju

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
64.3
-
 3,4-dimethoxybenzyl alcohol enzyme form VAO II Lentinus sajor-caju
82.2
-
 3,4-dimethoxybenzyl alcohol enzyme form VAO I Lentinus sajor-caju

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
5
-
-
 Lentinus sajor-caju

pH Range

pH Minimum pH Maximum Comment Organism
3.5 8 pH 3.5: about 70% of maximal activity, pH 8.0: about 65% of maximal activity Lentinus sajor-caju

Cofactor

Cofactor Comment Organism Structure
flavin prosthetic group Lentinus sajor-caju