KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.41 | - |
3,4-dimethoxybenzyl alcohol | enzyme form VAO I | Lentinus sajor-caju | |
0.46 | - |
3,4-dimethoxybenzyl alcohol | enzyme form VAO II | Lentinus sajor-caju |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
71000 | - |
x * 71000, SDS-PAGE | Lentinus sajor-caju |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | Lentinus sajor-caju | production of H2O2 during oxidation of lignin fragments | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Lentinus sajor-caju | - |
- |
- |
Posttranslational Modification | Comment | Organism |
---|---|---|
glycoprotein | - |
Lentinus sajor-caju |
Purification (Comment) | Organism |
---|---|
2 enzyme forms: VAO I and VAO II | Lentinus sajor-caju |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
culture medium | - |
Lentinus sajor-caju | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
30.7 | - |
- |
Lentinus sajor-caju |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2,4-dimethoxybenzyl alcohol + O2 | 50% of the activity with 3,4-dimethoxybenzyl alcohol, VAO I. 75% of the activity with 3,4-dimethoxybenzyl alcohol, VAO II | Lentinus sajor-caju | 2,4-dimethoxybenzyl aldehyde + H2O2 | - |
? | |
3,4-dimethoxybenzyl alcohol + O2 | i.e. veratryl alcohol | Lentinus sajor-caju | veratryl aldehyde + H2O2 | - |
? | |
3,5-dimethoxybenzyl alcohol + O2 | 7% of the activity with 3,4-dimethoxybenzyl alcohol, VAO I. 8% of the activity with 3,4-dimethoxybenzyl alcohol, VAO II | Lentinus sajor-caju | 3,5-dimethoxy benzaldehyde + H2O2 | - |
? | |
3-hydroxy-4-methoxybenzyl alcohol + O2 | 62% of the activity with 3,4-dimethoxybenzyl alcohol, VAO I. 71% of the activity with 3,4-dimethoxybenzyl alcohol, VAO II | Lentinus sajor-caju | 3-hydroxy-4-methoxybenzaldehyde + H2O2 | - |
? | |
3-methoxybenzyl alcohol + O2 | 19% of the activity with 3,4-dimethoxybenzyl alcohol, VAO I. 16% of the activity with 3,4-dimethoxybenzyl alcohol, VAO II | Lentinus sajor-caju | 3-methoxybenzaldehyde + H2O2 | - |
? | |
3-phenoxybenzyl alcohol + O2 | 35% of the activity with 3,4-dimethoxybenzyl alcohol, VAO I. 18% of the activity with 3,4-dimethoxybenzyl alcohol, VAO II | Lentinus sajor-caju | 3-phenoxybenzaldehyde + H2O2 | - |
? | |
4-methoxybenzyl alcohol + O2 | 200% of the activity with 3,4-dimethoxybenzyl alcohol, VAO I. 266% of the activity with 3,4-dimethoxybenzyl alcohol, VAO II | Lentinus sajor-caju | 4-methoxybenzaldehyde + H2O2 | - |
? | |
benzyl alcohol + O2 | 30% of the activity with 3,4-dimethoxybenzyl alcohol, VAO I. 25% of the activity with 3,4-dimethoxybenzyl alcohol, VAO II | Lentinus sajor-caju | benzaldehyde + H2O2 | - |
? | |
cinnamyl alcohol + O2 | oxidation at the gamma position, 77% of the activity with 3,4-dimethoxybenzyl alcohol, VAO I. 55% of the activity with 3,4-dimethoxybenzyl alcohol, VAO II | Lentinus sajor-caju | cinnamic aldehyde + H2O2 | - |
? | |
additional information | production of H2O2 during oxidation of lignin fragments | Lentinus sajor-caju | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 71000, SDS-PAGE | Lentinus sajor-caju |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
64.3 | - |
3,4-dimethoxybenzyl alcohol | enzyme form VAO II | Lentinus sajor-caju | |
82.2 | - |
3,4-dimethoxybenzyl alcohol | enzyme form VAO I | Lentinus sajor-caju |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
5 | - |
- |
Lentinus sajor-caju |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
3.5 | 8 | pH 3.5: about 70% of maximal activity, pH 8.0: about 65% of maximal activity | Lentinus sajor-caju |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
flavin | prosthetic group | Lentinus sajor-caju |