Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli BL21(DE3)pLysS cells | Rhodococcus sp. |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.032 | - |
cholesterol | pH 7.0, 50°C | Rhodococcus sp. |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
55000 | - |
SDS-PAGE | Rhodococcus sp. |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Rhodococcus sp. | Q157H4 | - |
- |
Rhodococcus sp. PTCC 1633 | Q157H4 | - |
- |
Purification (Comment) | Organism |
---|---|
to purify the recombinant enzyme, 500 mg cholesterol is added to 50 ml of crude extract, and then incubated for 2 h at 4°C followed by centrifugation at 10000g for 10 min | Rhodococcus sp. |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
beta-sitosterol + O2 | 64% activity compared to cholesterol | Rhodococcus sp. | stigmast-5-en-3-one + H2O2 | - |
? | |
beta-sitosterol + O2 | 64% activity compared to cholesterol | Rhodococcus sp. PTCC 1633 | stigmast-5-en-3-one + H2O2 | - |
? | |
beta-stigmasterol + O2 | 33% activity compared to cholesterol | Rhodococcus sp. | stigmasta-5,22-dien-3-one + H2O2 | - |
? | |
beta-stigmasterol + O2 | 33% activity compared to cholesterol | Rhodococcus sp. PTCC 1633 | stigmasta-5,22-dien-3-one + H2O2 | - |
? | |
cholesterol + O2 | 100% activity | Rhodococcus sp. | cholest-4-en-3-one + H2O2 | - |
? | |
cholesterol + O2 | 100% activity | Rhodococcus sp. PTCC 1633 | cholest-4-en-3-one + H2O2 | - |
? | |
additional information | no activity with cholic acid, progesterone and 4-androsten-3,17-dione | Rhodococcus sp. | ? | - |
? | |
additional information | no activity with cholic acid, progesterone and 4-androsten-3,17-dione | Rhodococcus sp. PTCC 1633 | ? | - |
? |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
50 | - |
- |
Rhodococcus sp. |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
45 | 55 | after 1 h of incubation, more than 70 and 50% of activity remains at 45 and 55°C, respectively | Rhodococcus sp. |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7 | - |
- |
Rhodococcus sp. |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
5.5 | 8 | relative high activities in a broad pH range of 6.0-8.0, however activity rapidly declines under pH 5.5 | Rhodococcus sp. |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
FAD | - |
Rhodococcus sp. |
Organism | Comment | pI Value Maximum | pI Value |
---|---|---|---|
Rhodococcus sp. | pI-value about 9.0, isoelectric focusing | - |
9 |