Literature summary for 1.1.3.6 extracted from

Saxena, U.; Goswami, P.
Silk mat as bio-matrix for the immobilization of cholesterol oxidase (2010), Appl. Biochem. Biotechnol., 162, 1122-1131.

Search for more literature for 1.1.3.6

General Stability

General Stability	Organism
the ChOx-immobilized silk mat maintains its initial activity till the fourth successive measurement and retains about 50% of its initial activity at the end of 25 measurements	Pseudomonas fluorescens

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
0.2123	-	cholesterol	apparent value, free enzyme, in 50 mM potassium phosphate buffer, pH 7.5, 30°C	Pseudomonas fluorescens
0.2599	-	cholesterol	apparent value, silk mat-immobilized enzyme, in 50 mM potassium phosphate buffer, pH 7.5, 30°C	Pseudomonas fluorescens

Organism

Organism	UniProt	Comment	Textmining
Pseudomonas fluorescens	-	-	-

Source Tissue

Source Tissue	Comment	Organism	Textmining

Storage Stability

Storage Stability	Organism
4°C, ChOx-immobilized silk mat, when the measurements are made at 3 days interval the immobilized ChOx can retain about 50% of its original activity after 45 days of storage and when the same carried out with 7 days interval, about 70% of its original activity is retained till 60 days	Pseudomonas fluorescens

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
cholesterol + O2	-	Pseudomonas fluorescens	cholest-4-en-3-one + H2O2	-	ir

Synonyms

Synonyms	Comment	Organism
ChOx	-	Pseudomonas fluorescens

Temperature Optimum [°C]

Temperature Optimum [°C]	Temperature Optimum Maximum [°C]	Comment	Organism
30	-	silk mat-immobilized enzyme	Pseudomonas fluorescens

Temperature Range [°C]

Temperature Minimum [°C]	Temperature Maximum [°C]	Comment	Organism
25	45	the oxygen depletion rate is increased with increasing temperature from 25°C to 40°C but it decreases sharply as the temperature increases further to 45°C	Pseudomonas fluorescens

pH Optimum

pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
7.5	-	silk mat-immobilized enzyme	Pseudomonas fluorescens

pH Range

pH Minimum	pH Maximum	Comment	Organism
4	7.5	the activity increases with increasing pH value from 4.0 to 7.5 and then declines as pH increases further	Pseudomonas fluorescens

Cofactor

Cofactor	Comment	Organism	Structure
FAD	-	Pseudomonas fluorescens

kcat/KM [mM/s]

kcat/KM Value [1/mMs^-1]	kcat/KM Value Maximum [1/mMs^-1]	Substrate	Comment	Organism	Structure
0.0013	-	cholesterol	apparent value, silk mat-immobilized enzyme, in 50 mM potassium phosphate buffer, pH 7.5, 30°C	Pseudomonas fluorescens

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Release 2023.1 (January 2023)