Literature summary for 1.1.3.6 extracted from

Park, M.S.; Kwon, B.; Shim, J.J.; Huh, C.S.; Ji, G.E.
Heterologous expression of cholesterol oxidase in Bifidobacterium longum under the control of 16S rRNA gene promoter of bifidobacteria (2008), Biotechnol. Lett., 30, 165-172.

Cloned(Commentary)

Cloned (Comment)	Organism
construction of a constitutive high-level-expression vector for the genus Bifidobacterium to express cholesterol oxidase from Streptomyces coelicola. The promoter region of the 16S rRNA gene is amplified by inverse PCR and used for the construction of pBES16PR. The optimal ribosome-binding site for Bifidobacterium is incorporated in pBES16PR. pBES16PR-CHOL with the structural gene for cholesterol oxidase under the control of the 16S rRNA promoter is used to transform Bifidobacterium longum. The gene is successfully expressed and high level of cholesterol oxidase activity is obtained in Bifidobacterium longum	Streptomyces coelicolor

Cloned (Comment)

Organism

construction of a constitutive high-level-expression vector for the genus Bifidobacterium to express cholesterol oxidase from Streptomyces coelicola. The promoter region of the 16S rRNA gene is amplified by inverse PCR and used for the construction of pBES16PR. The optimal ribosome-binding site for Bifidobacterium is incorporated in pBES16PR. pBES16PR-CHOL with the structural gene for cholesterol oxidase under the control of the 16S rRNA promoter is used to transform Bifidobacterium longum. The gene is successfully expressed and high level of cholesterol oxidase activity is obtained in Bifidobacterium longum

Streptomyces coelicolor

Organism

Organism	UniProt	Comment	Textmining
Streptomyces coelicolor	-	-	-

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Release 2023.1 (January 2023)