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Literature summary for 1.1.3.6 extracted from

  • Doukyu, N.; Aono, R.
    Cloning, sequence analysis and expression of a gene encoding an organic solvent- and detergent-tolerant cholesterol oxidase of Burkholderia cepacia strain ST-200 (2001), Appl. Microbiol. Biotechnol., 57, 146-152.
    View publication on PubMed
Search for more literature for 1.1.3.6

Activating Compound

Activating Compound Comment Organism Structure
acetone 3.8fold increase in activity, 1 ml solvent added to 2 ml 50 mM phosphate buffer Burkholderia cepacia
propan-2-ol 2fold increase in activity, 1 ml solvent added to 2 ml 50 mM phosphate buffer Burkholderia cepacia

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Burkholderia cepacia

Inhibitors

Inhibitors Comment Organism Structure
additional information not inhibited by 1% Triton X-100, 1% Tween 20, 1% sodium cholate and 1% CHAPS Brevibacterium sterolicum
additional information not inhibited by 1% Triton X-100, 1% Tween 20, 1% sodium cholate and 1% CHAPS Burkholderia cepacia
additional information not inhibited by 1% Triton X-100, 1% Tween 20, 1% sodium cholate and 1% CHAPS Nocardia erythropolis
additional information not inhibited by 1% Triton X-100, 1% Tween 20, 1% sodium cholate and 1% CHAPS Pseudomonas sp.
Sarcosyl 1%, 88% inhibition Brevibacterium sterolicum
Sarcosyl 1%, 56% inhibition Streptomyces sp.
SDS 0.1% complete inhibition Brevibacterium sterolicum
SDS 0.1%, 20% inhibition, 1%, 41% inhibition Burkholderia cepacia
SDS 0.1% complete inhibition Nocardia erythropolis
SDS 0.1% complete inhibition Pseudomonas sp.

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
cholesterol + O2 Pseudomonas sp.
-
 cholest-5-en-3-one + H2O2
-
 ?
cholesterol + O2 Streptomyces sp.
-
 cholest-5-en-3-one + H2O2
-
 ?
cholesterol + O2 Burkholderia cepacia
-
 cholest-5-en-3-one + H2O2
-
 ?
cholesterol + O2 Nocardia erythropolis
-
 cholest-5-en-3-one + H2O2
-
 ?
cholesterol + O2 Brevibacterium sterolicum
-
 cholest-5-en-3-one + H2O2
-
 ?
cholesterol + O2 Burkholderia cepacia ST-200
-
 cholest-5-en-3-one + H2O2
-
 ?

Organism

Organism UniProt Comment Textmining
Brevibacterium sterolicum
-
-
-
Burkholderia cepacia
-
-
-
Burkholderia cepacia ST-200
-
-
-
Nocardia erythropolis
-
-
-
Pseudomonas sp.
-
-
-
Streptomyces sp.
-
-
-

Purification (Commentary)

Purification (Comment) Organism
ammonium sulfate, DEAE-cellulose, butyl-toyopearl Burkholderia cepacia

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
16.9
-
-
 Burkholderia cepacia

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
cholesterol + O2
-
 Pseudomonas sp. cholest-4-en-3-one + H2O2
-
 ?
cholesterol + O2
-
 Streptomyces sp. cholest-4-en-3-one + H2O2
-
 ?
cholesterol + O2
-
 Burkholderia cepacia cholest-4-en-3-one + H2O2
-
 ?
cholesterol + O2
-
 Nocardia erythropolis cholest-4-en-3-one + H2O2
-
 ?
cholesterol + O2
-
 Brevibacterium sterolicum cholest-4-en-3-one + H2O2
-
 ?
cholesterol + O2
-
 Burkholderia cepacia ST-200 cholest-4-en-3-one + H2O2
-
 ?
cholesterol + O2
-
 Pseudomonas sp. cholest-5-en-3-one + H2O2
-
 ?
cholesterol + O2
-
 Streptomyces sp. cholest-5-en-3-one + H2O2
-
 ?
cholesterol + O2
-
 Burkholderia cepacia cholest-5-en-3-one + H2O2
-
 ?
cholesterol + O2
-
 Nocardia erythropolis cholest-5-en-3-one + H2O2
-
 ?
cholesterol + O2
-
 Brevibacterium sterolicum cholest-5-en-3-one + H2O2
-
 ?
cholesterol + O2
-
 Burkholderia cepacia ST-200 cholest-5-en-3-one + H2O2
-
 ?

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
70
-
 20% activity lost after incubation for 30 min Burkholderia cepacia