Activating Compound | Comment | Organism | Structure |
---|---|---|---|
dodecylpoly(ethylene glycol ether)9-10 | trivial name thesit, 0.1 mM cholesterol: increase in enzyme activity in the presence of 0.1% to 2% thesit, 0.34 mM cholesterol: sharp decrease with increasing thesit concentrations | Streptomyces hygroscopicus | |
dodecylpoly(ethylene glycol ether)9-10 | trivial name thesit, 0.1 mM cholesterol: increase in enzyme activity in the presence of 0.1% to 2% thesit, 0.34 mM cholesterol: sharp decrease with increasing thesit concentrations | Brevibacterium sterolicum | |
phosphate ions | marked increase above 90 mM | Streptomyces hygroscopicus | |
phosphate ions | marked increase above 90 mM | Brevibacterium sterolicum | |
propan-2-ol | maximal enzyme activity with 10%(v/v) in the presence of 500 mM potassium phosphate, decrease in activity in the presence of 50 mM potassium phosphate | Streptomyces hygroscopicus |
General Stability | Organism |
---|---|
65% activity remains after 24 h at 25°C in 50 mM potassium phosphate, pH 7.5, enzyme is completely stable for 300 min in the presence of 1% propan-2-ol and 1% thesit as well as in the presence of 10% propan-2-ol and 8% thesit | Streptomyces hygroscopicus |
65% activity remains after 24 h at 25°C in 50 mM potassium phosphate, pH 7.5, enzyme is completely stable for 300 min in the presence of 1% propan-2-ol and 1% thesit as well as in the presence of 10% propan-2-ol and 8% thesit | Brevibacterium sterolicum |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Triton X-100 | strong decrease in enzyme activity at concentration of 0.2% Triton X-100 or higher | Brevibacterium sterolicum | |
Triton X-100 | strong decrease in enzyme activity at concentration of 0.2% Triton X-100 or higher | Streptomyces hygroscopicus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.4 | - |
dehydroepiandrosterone | - |
Brevibacterium sterolicum |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
cholesterol + O2 | Streptomyces hygroscopicus | - |
cholest-5-en-3-one + H2O2 | - |
? | |
cholesterol + O2 | Brevibacterium sterolicum | - |
cholest-5-en-3-one + H2O2 | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Brevibacterium sterolicum | - |
- |
- |
Streptomyces hygroscopicus | - |
- |
- |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
cholesterol + O2 = cholest-5-en-3-one + H2O2 | bifunctional enzyme, catalyzes both the oxidation of DELTA5-ene-3beta hydroxysteroids with a trans A-B ring junction to the corresponding DELTA5-3-ketosteroid with the reduction of oxygen to hydrogen peroxide, and the isomerization to the DELTA4-3-ketosteroid | Streptomyces hygroscopicus | |
cholesterol + O2 = cholest-5-en-3-one + H2O2 | bifunctional enzyme, catalyzes both the oxidation of DELTA5-ene-3beta hydroxysteroids with a trans A-B ring junction to the corresponding DELTA5-3-ketosteroid with the reduction of oxygen to hydrogen peroxide, and the isomerization to the DELTA4-3-ketosteroid | Brevibacterium sterolicum |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
cholesterol + O2 | - |
Streptomyces hygroscopicus | cholest-4-en-3-one + H2O2 | - |
? | |
cholesterol + O2 | - |
Brevibacterium sterolicum | cholest-4-en-3-one + H2O2 | - |
? | |
cholesterol + O2 | - |
Streptomyces hygroscopicus | cholest-5-en-3-one + H2O2 | - |
? | |
cholesterol + O2 | - |
Brevibacterium sterolicum | cholest-5-en-3-one + H2O2 | - |
? |