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Literature summary for 1.1.3.6 extracted from

  • Sojo, M.; Bru, R.; Lopez-Molina, D.; Garcia-Carmona, F.; Arguelles, J.C.
    Cell-linked and extracellular cholesterol oxidase activities from Rhodococcus erythropolis. Isolation and physiological characterization (1997), Appl. Microbiol. Biotechnol., 47, 583-589.
    View publication on PubMed

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.031
-
cholesterol cell-linked and extracellular enzyme Rhodococcus erythropolis

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
cholesterol + O2 Rhodococcus erythropolis
-
cholest-5-en-3-one + H2O2
-
?
cholesterol + O2 Nocardia rhodochrous
-
cholest-5-en-3-one + H2O2
-
?

Organism

Organism UniProt Comment Textmining
Nocardia rhodochrous
-
renamed as Rhodococcus rhodochrous
-
Rhodococcus erythropolis
-
ATCC 25544, de novo enzyme synthesis is induced by cholesterol
-

Reaction

Reaction Comment Organism Reaction ID
cholesterol + O2 = cholest-5-en-3-one + H2O2 bifunctional enzyme, catalyzes both the oxidation of DELTA5-ene-3beta hydroxysteroids with a trans A-B ring junction to the corresponding DELTA5-3-ketosteroid with the reduction of oxygen to hydrogen peroxide, and the isomerization to the DELTA4-3-ketosteroid Rhodococcus erythropolis
cholesterol + O2 = cholest-5-en-3-one + H2O2 bifunctional enzyme, catalyzes both the oxidation of DELTA5-ene-3beta hydroxysteroids with a trans A-B ring junction to the corresponding DELTA5-3-ketosteroid with the reduction of oxygen to hydrogen peroxide, and the isomerization to the DELTA4-3-ketosteroid Nocardia rhodochrous

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
cholesterol + O2
-
Rhodococcus erythropolis cholest-4-en-3-one + H2O2
-
?
cholesterol + O2
-
Nocardia rhodochrous cholest-4-en-3-one + H2O2
-
?
cholesterol + O2
-
Rhodococcus erythropolis cholest-5-en-3-one + H2O2
-
?
cholesterol + O2
-
Nocardia rhodochrous cholest-5-en-3-one + H2O2
-
?