Application | Comment | Organism |
---|---|---|
diagnostics | used in an automatic glucose assay kit in conjunction with catalase and chiefly in biosensors for the detection and estimation of glucose in industrial solutions and in body fluids such as blood and urine | Aspergillus niger |
food industry | food and beverage additive, used for low alcohol wine production, used for glucose removal from dried egg, improvement of color, flavor, and shelf life of food materials, oxygen removal from fruit juices, canned beverages, and from mayonnaise to prevent rancidity, used as ingredient of toothpaste, for the production of gluconic acid, and as a food preservative | Aspergillus niger |
synthesis | GOD is used as a commercial source of gluconic acid, which can be produced by the hydrolysis of delta-glucono-1, 5-lactone, the endproduct of GOD catalysis | Aspergillus niger |
Cloned (Comment) | Organism |
---|---|
expressed in Saccharomyces cerevisiae | Aspergillus niger |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
4-chloromercuribenzoate | - |
Aspergillus niger | |
Ag+ | - |
Aspergillus niger | |
Cu2+ | - |
Aspergillus niger | |
dimedone | - |
Aspergillus niger | |
Hg2+ | - |
Aspergillus niger | |
hydrazine | - |
Aspergillus niger | |
hydroxylamine | - |
Aspergillus niger | |
phenylhydrazine | - |
Aspergillus niger | |
sodium bisulfate | - |
Aspergillus niger |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
33 | - |
beta-D-glucose | - |
Aspergillus niger |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
152000 | - |
SDS-PAGE | Aspergillus niger |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Aspergillus niger | - |
- |
- |
Purification (Comment) | Organism |
---|---|
ammonium sulfate precipitation, ion exchange chromatography, and gel filtration | Aspergillus niger |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
mycelium | - |
Aspergillus niger | - |
Storage Stability | Organism |
---|---|
-20°C, lyophilised enzyme, minimum of 6 months, remains stable | Aspergillus niger |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2-deoxy-D-glucose + O2 + H2O | low GOD activity | Aspergillus niger | 2-deoxy-D-glucono-1,5-lactone + H2O2 | - |
? | |
beta-D-glucose + O2 + H2O | GOD is highly specific for the beta-anomer of D-glucose | Aspergillus niger | D-glucono-1,5-lactone + H2O2 | - |
ir | |
D-galactose + O2 + H2O | low GOD activity | Aspergillus niger | ? | - |
? | |
D-mannose + O2 + H2O | low GOD activity | Aspergillus niger | ? | - |
? | |
additional information | alpha-D-glucose is not a suitable substrate | Aspergillus niger | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
beta-D-glucose:oxygen 1-oxidoreductase | - |
Aspergillus niger |
GOD | - |
Aspergillus niger |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
40 | 60 | - |
Aspergillus niger |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
GOD has half-life of approximately 30 min at 37°C, immobilized GOD is more effective for applications at 37°C | Aspergillus niger |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
5 | 6 | - |
Aspergillus niger |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
3.5 | 6.5 | - |
Aspergillus niger |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
FAD | - |
Aspergillus niger |