KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0368 | - |
2,6-dichloroindophenol | - |
Talaromyces pinophilus | |
6.2 | - |
beta-D-glucose | - |
Talaromyces pinophilus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
77700 | - |
2 * 77700, SDS-PAGE, confirmed by electron-microscopic examinations | Talaromyces pinophilus |
148000 | - |
gel filtration on Superdex 200 | Talaromyces pinophilus |
152800 | - |
electron microscopy | Talaromyces pinophilus |
159000 | - |
calculated value based on Stokes' radius and sedimentation coefficient | Talaromyces pinophilus |
160000 | - |
gel filtration on Sephacryl S-300 | Talaromyces pinophilus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Talaromyces pinophilus | - |
- |
- |
Purification (Comment) | Organism |
---|---|
using an extraction step of the mycelium mass at pH 3.0, cation exchange chromatography on S-Sepharose and gel filtration on Sephacryl S-300 | Talaromyces pinophilus |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
- |
Talaromyces pinophilus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
beta-D-glucose + 2,6-dichlorophenol indophenol | - |
Talaromyces pinophilus | D-glucono-1,5-lactone + ? | - |
? | |
beta-D-glucose + O2 + H2O | specific for D-glucose, 2,6-dichloroindophenol can act as artificial electron acceptor | Talaromyces pinophilus | D-glucono-1,5-lactone + H2O2 | - |
? |
Subunits | Comment | Organism |
---|---|---|
dimer | 2 * 77700, SDS-PAGE, confirmed by electron-microscopic examinations | Talaromyces pinophilus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | 50 | in 100 mM potassium phosphate, pH 5.0 | Talaromyces pinophilus |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
40 | - |
at pH 2-4 and pH 7-8, half life: less than 1 day | Talaromyces pinophilus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
4 | 6 | - |
Talaromyces pinophilus |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
3 | - |
50% activity | Talaromyces pinophilus |
5 | - |
extremely stable, no loss of activity detected after 300 days at 4°C and 30°C | Talaromyces pinophilus |
6 | - |
extremely stable | Talaromyces pinophilus |
7.5 | - |
50% activity | Talaromyces pinophilus |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
FAD | 1.76 nmol FAD per nmol enzyme | Talaromyces pinophilus |