General Stability | Organism |
---|---|
SDS, 1.5%, 2-mercaptoethanol, 55°C, residual activity: 24% | Aspergillus niger |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Iron | the enzyme contains 2 mol of iron per 160000 Da | Aspergillus niger |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
79000 | - |
2 * 79000, SDS-PAGE | Aspergillus niger |
158000 | 160000 | gel filtration | Aspergillus niger |
160000 | - |
gel filtration | Aspergillus niger |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
beta-D-glucose + O2 + H2O | Aspergillus niger | - |
D-glucono-1,5-lactone + H2O2 | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Aspergillus niger | - |
- |
- |
Posttranslational Modification | Comment | Organism |
---|---|---|
glycoprotein | the enzyme contains 74% protein, 16.4% neutral sugar and 2.4% amino sugar, major component: mannose | Aspergillus niger |
Purification (Comment) | Organism |
---|---|
from commercial preparation using DEAE-cellulose chromatography and Sephadex G-200 gel filtration | Aspergillus niger |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
commercial preparation | - |
Aspergillus niger | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
172 | - |
purified enzyme | Aspergillus niger |
Storage Stability | Organism |
---|---|
lyophilized, over P2O5 | Aspergillus niger |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
beta-D-glucose + O2 + H2O | - |
Aspergillus niger | D-glucono-1,5-lactone + H2O2 | - |
? |
Subunits | Comment | Organism |
---|---|---|
dimer | 2 * 79000, SDS-PAGE | Aspergillus niger |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
FAD | - |
Aspergillus niger | |
Flavin-hypoxanthine dinucleotide | FHD, can substitute FAD | Aspergillus niger |