Cloned (Comment) | Organism |
---|---|
gene LdALO, DNA and amino acid sequence determination and analysis, phylogenetic tree, expression of maltose binding protein-tagged LdALO in Escherichia coli strain BL21 (DE3), overexpressioin Leishmania donovani | Leishmania donovani |
Protein Variants | Comment | Organism |
---|---|---|
additional information | overexpression of LdALO in Leishmannia donovani results in better ability of survival of the parasite within the host in comparison to the vector transfectants | Leishmania donovani |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
HgCl2 | 68% inhibition at 0.05 mM, complete inhibition at 1 mM | Leishmania donovani | |
N-ethylmaleimide | complete inhibition at 0.05 mM | Leishmania donovani | |
ZnCl2 | 17% inhibition at 0.05 mM, 75% inhibition at 1 mM | Leishmania donovani |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | Michaelis-Menten kinetics, overview | Leishmania donovani | |
0.0393 | - |
D-arabinono-1,4-lactone | pH 7.5, 22°C | Leishmania donovani |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
glycosome | - |
Leishmania donovani | 20015 | - |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
57000 | - |
x * 57000, SDS-PAGE | Leishmania donovani |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
D-arabinono-1,4-lactone + O2 | Leishmania donovani | - |
dehydro-D-arabinono-1,4-lactone + H2O2 | - |
? | |
D-arabinono-1,4-lactone + O2 | Leishmania donovani AG83 | - |
dehydro-D-arabinono-1,4-lactone + H2O2 | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Leishmania donovani | C8CCV9 | - |
- |
Leishmania donovani AG83 | C8CCV9 | - |
- |
Purification (Comment) | Organism |
---|---|
recombinant maltose binding protein-tagged LdALO from Escherichia coli strain BL21 (DE3) by amylose affinity chromatography, Factor Xa cleavage of maltose binding protein-tag | Leishmania donovani |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
D-arabinono-1,4-lactone + O2 | - |
Leishmania donovani | dehydro-D-arabinono-1,4-lactone + H2O2 | - |
? | |
D-arabinono-1,4-lactone + O2 | - |
Leishmania donovani AG83 | dehydro-D-arabinono-1,4-lactone + H2O2 | - |
? | |
additional information | no activity with L-gulono-1,4-lactone | Leishmania donovani | ? | - |
? | |
additional information | no activity with L-gulono-1,4-lactone | Leishmania donovani AG83 | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 57000, SDS-PAGE | Leishmania donovani |
Synonyms | Comment | Organism |
---|---|---|
ALO | - |
Leishmania donovani |
arabino-1,4-lactone oxidase | - |
Leishmania donovani |
arabinonolactone oxidase | - |
Leishmania donovani |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
22 | - |
assay at room temperature | Leishmania donovani |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
10.8 | - |
D-arabinono-1,4-lactone | pH 7.5, 22°C | Leishmania donovani |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | - |
assay at | Leishmania donovani |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
FAD | essentially required | Leishmania donovani |
General Information | Comment | Organism |
---|---|---|
evolution | the enzyme belongs to the family of flavoprotein aldonolactone oxidase/dehydrogenases | Leishmania donovani |
metabolism | the enzyme catalyzes the last step in the biosynthesis of L-ascorbic acid involving the conversion of an aldonolactone substrate to ascorbate (or analogues) | Leishmania donovani |
additional information | cysteine residues are required for enzyme activity | Leishmania donovani |
physiological function | D-arabinono-1,4-lactone oxidase is required for synthesizing ascorbate in Leishmania | Leishmania donovani |