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Literature summary for 1.1.3.37 extracted from

  • Biyani, N.; Madhubala, R.
    Leishmania donovani encodes a functional enzyme involved in vitamin C biosynthesis: arabino-1,4-lactone oxidase (2011), Mol. Biochem. Parasitol., 180, 76-85.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
gene LdALO, DNA and amino acid sequence determination and analysis, phylogenetic tree, expression of maltose binding protein-tagged LdALO in Escherichia coli strain BL21 (DE3), overexpressioin Leishmania donovani Leishmania donovani

Protein Variants

Protein Variants Comment Organism
additional information overexpression of LdALO in Leishmannia donovani results in better ability of survival of the parasite within the host in comparison to the vector transfectants Leishmania donovani

Inhibitors

Inhibitors Comment Organism Structure
HgCl2 68% inhibition at 0.05 mM, complete inhibition at 1 mM Leishmania donovani
N-ethylmaleimide complete inhibition at 0.05 mM Leishmania donovani
ZnCl2 17% inhibition at 0.05 mM, 75% inhibition at 1 mM Leishmania donovani

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
additional information Michaelis-Menten kinetics, overview Leishmania donovani
0.0393
-
D-arabinono-1,4-lactone pH 7.5, 22°C Leishmania donovani

Localization

Localization Comment Organism GeneOntology No. Textmining
glycosome
-
Leishmania donovani 20015
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
57000
-
x * 57000, SDS-PAGE Leishmania donovani

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
D-arabinono-1,4-lactone + O2 Leishmania donovani
-
dehydro-D-arabinono-1,4-lactone + H2O2
-
?
D-arabinono-1,4-lactone + O2 Leishmania donovani AG83
-
dehydro-D-arabinono-1,4-lactone + H2O2
-
?

Organism

Organism UniProt Comment Textmining
Leishmania donovani C8CCV9
-
-
Leishmania donovani AG83 C8CCV9
-
-

Purification (Commentary)

Purification (Comment) Organism
recombinant maltose binding protein-tagged LdALO from Escherichia coli strain BL21 (DE3) by amylose affinity chromatography, Factor Xa cleavage of maltose binding protein-tag Leishmania donovani

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
D-arabinono-1,4-lactone + O2
-
Leishmania donovani dehydro-D-arabinono-1,4-lactone + H2O2
-
?
D-arabinono-1,4-lactone + O2
-
Leishmania donovani AG83 dehydro-D-arabinono-1,4-lactone + H2O2
-
?
additional information no activity with L-gulono-1,4-lactone Leishmania donovani ?
-
?
additional information no activity with L-gulono-1,4-lactone Leishmania donovani AG83 ?
-
?

Subunits

Subunits Comment Organism
? x * 57000, SDS-PAGE Leishmania donovani

Synonyms

Synonyms Comment Organism
ALO
-
Leishmania donovani
arabino-1,4-lactone oxidase
-
Leishmania donovani
arabinonolactone oxidase
-
Leishmania donovani

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
22
-
assay at room temperature Leishmania donovani

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
10.8
-
D-arabinono-1,4-lactone pH 7.5, 22°C Leishmania donovani

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5
-
assay at Leishmania donovani

Cofactor

Cofactor Comment Organism Structure
FAD essentially required Leishmania donovani

General Information

General Information Comment Organism
evolution the enzyme belongs to the family of flavoprotein aldonolactone oxidase/dehydrogenases Leishmania donovani
metabolism the enzyme catalyzes the last step in the biosynthesis of L-ascorbic acid involving the conversion of an aldonolactone substrate to ascorbate (or analogues) Leishmania donovani
additional information cysteine residues are required for enzyme activity Leishmania donovani
physiological function D-arabinono-1,4-lactone oxidase is required for synthesizing ascorbate in Leishmania Leishmania donovani