Activating Compound | Comment | Organism | Structure |
---|---|---|---|
SDS | slightly accelerates activity | Pseudomonas sp. |
Application | Comment | Organism |
---|---|---|
analysis | enzyme may by useful for measurement of N-acetyl-beta-D-glucosaminidase | Pseudomonas sp. |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Cd2+ | weak | Pseudomonas sp. | |
Cu2+ | - |
Pseudomonas sp. | |
Hg2+ | - |
Pseudomonas sp. | |
Mn2+ | - |
Pseudomonas sp. | |
Ni2+ | - |
Pseudomonas sp. | |
oxalate | - |
Pseudomonas sp. | |
PCMB | - |
Pseudomonas sp. | |
Zn2+ | - |
Pseudomonas sp. |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.1 | - |
N-acetyl-D-galactosamine | - |
Pseudomonas sp. | |
0.24 | - |
N-acetyl-D-glucosamine | - |
Pseudomonas sp. | |
2.6 | - |
N-Glycolyl-D-glucosamine | - |
Pseudomonas sp. | |
10 | - |
D-galactosamine | - |
Pseudomonas sp. | |
18 | - |
N,N-diacetylchitobiose | - |
Pseudomonas sp. | |
33 | - |
N-acetyl muramic acid | - |
Pseudomonas sp. | |
40 | - |
D-glucosamine | - |
Pseudomonas sp. | |
40 | - |
N-acetyl-D-mannosamine | - |
Pseudomonas sp. | |
59 | - |
D-mannose | - |
Pseudomonas sp. | |
170 | - |
D-galactose | - |
Pseudomonas sp. | |
290 | - |
D-glucose | - |
Pseudomonas sp. |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
22000 | - |
1 * 63000 + 1 * 44000 + 1 * 36000 + 1 * 22000, SDS-PAGE | Pseudomonas sp. |
36000 | - |
1 * 63000 + 1 * 44000 + 1 * 36000 + 1 * 22000, SDS-PAGE | Pseudomonas sp. |
44000 | - |
1 * 63000 + 1 * 44000 + 1 * 36000 + 1 * 22000, SDS-PAGE | Pseudomonas sp. |
63000 | - |
1 * 63000 + 1 * 44000 + 1 * 36000 + 1 * 22000, SDS-PAGE | Pseudomonas sp. |
170000 | - |
gel filtration | Pseudomonas sp. |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Pseudomonas sp. | - |
- |
- |
Pseudomonas sp. 15-1 | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Pseudomonas sp. |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
70.4 | - |
N-acetyl-D-glucosamine | Pseudomonas sp. |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
D-galactosamine + O2 | 35% of the activity with N-acetyl-D-glucosamine | Pseudomonas sp. | ? | - |
? | |
D-galactose + O2 | 0.43% of the activity with N-acetyl-D-glucosamine | Pseudomonas sp. | D-galactono-1,5-lactone + H2O2 | - |
? | |
D-galactose + O2 | 0.43% of the activity with N-acetyl-D-glucosamine | Pseudomonas sp. 15-1 | D-galactono-1,5-lactone + H2O2 | - |
? | |
D-glucosamine + O2 | 14% of the activity with N-acetyl-D-glucosamine | Pseudomonas sp. | ? | - |
? | |
D-glucose + O2 | 0.3% of the activity with N-acetyl-D-glucosamine | Pseudomonas sp. | D-gluconolactone + H2O2 | - |
? | |
D-mannosamine + O2 | 0.03% of the activity with N-acetyl-D-glucosamine | Pseudomonas sp. | ? | - |
? | |
D-mannose + O2 | 0.38% of the activity with N-acetyl-D-glucosamine | Pseudomonas sp. | D-mannonolactone + H2O2 | - |
? | |
N,N-diacetylchitobiose + O2 | 31% of the activity with N-acetyl-D-glucosamine | Pseudomonas sp. | ? | - |
? | |
N-acetyl-beta-D-glucosamine + O2 | alpha-anomer is not oxidized | Pseudomonas sp. | N-acetyl-D-glucosaminolactone + H2O2 | in presence of H2O: N-acetyl-D-glucosaminate + H2O2 | ? | |
N-acetyl-beta-D-glucosamine + O2 | alpha-anomer is not oxidized | Pseudomonas sp. 15-1 | N-acetyl-D-glucosaminolactone + H2O2 | in presence of H2O: N-acetyl-D-glucosaminate + H2O2 | ? | |
N-acetyl-D-galactosamine + O2 | 98% of the activity with N-acetyl-D-glucosamine | Pseudomonas sp. | N-acetyl-D-galactosaminolactone + H2O2 | - |
? | |
N-acetyl-D-galactosamine + O2 | 98% of the activity with N-acetyl-D-glucosamine | Pseudomonas sp. 15-1 | N-acetyl-D-galactosaminolactone + H2O2 | - |
? | |
N-acetyl-D-glucosamine + O2 | - |
Pseudomonas sp. | N-acetyl-D-glucosaminate + H2O2 | - |
r | |
N-acetyl-D-mannosamine + O2 | 4.9% of the activity with N-acetyl-D-glucosamine | Pseudomonas sp. | N-acetyl-D-mannosaminolactone + H2O2 | - |
? | |
N-acetyl-D-mannosamine + O2 | 4.9% of the activity with N-acetyl-D-glucosamine | Pseudomonas sp. 15-1 | N-acetyl-D-mannosaminolactone + H2O2 | - |
? | |
N-acetylmuramic acid + O2 | 44% of the activity with N-acetyl-D-glucosamine | Pseudomonas sp. | ? | - |
? | |
N-glycolyl-D-glucosamine + O2 | 72% of the activity with N-acetyl-D-glucosamine | Pseudomonas sp. | N-glycolyl-D-glucosaminolactone + H2O2 | - |
? | |
N-glycolyl-D-glucosamine + O2 | 72% of the activity with N-acetyl-D-glucosamine | Pseudomonas sp. 15-1 | N-glycolyl-D-glucosaminolactone + H2O2 | - |
? |
Subunits | Comment | Organism |
---|---|---|
tetramer | 1 * 63000 + 1 * 44000 + 1 * 36000 + 1 * 22000, SDS-PAGE | Pseudomonas sp. |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
45 | - |
10 min, stable | Pseudomonas sp. |
70 | - |
100% remaining activity after 10 min | Pseudomonas sp. |
83 | - |
50% remaining activity after 10 min | Pseudomonas sp. |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8 | - |
- |
Pseudomonas sp. |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
5 | 11 | pH 5.0: about 70% of maximal activity, pH 11: about 50% of maximal activity | Pseudomonas sp. |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
3 | 9 | stable at 45°C for 10 min | Pseudomonas sp. |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
additional information | probably contains unidentified flavin component | Pseudomonas sp. |