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Literature summary for 1.1.3.21 extracted from

  • Streitenberger, S.A.; Lopez-Mas, J.A.; Sanchez-Ferrer, A.; Garcia-Carmona, J.A.
    Highly efficient Aerococcus viridians L-alpha-glycerophosphate oxidase production in the presence of H2O2-decomposing agent: purification and kinetic characterization (2001), Appl. Microbiol. Biotechnol., 57, 329-333.
    View publication on PubMed

Inhibitors

Inhibitors Comment Organism Structure
benzoylformic acid strain CETC 978, competitive inhibition Aerococcus viridans
glyoxylic acid strain CETC 978, competitive inhibition Aerococcus viridans
methylglyoxal strain CETC 978, 277 mM, complete inactivation after 50 min, in the presence of 30 mM benzoylformic acid complete inactivation after 20 min Aerococcus viridans
additional information strain CETC 978, not inhibited by 20-80 mM fructose-1-phosphate or fructose-6-phosphate Aerococcus viridans

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.3
-
sn-glycerol 3-phosphate strain CETC 978 Aerococcus viridans

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
63000
-
strain CETC 978, x * 63000, SDS-PAGE Aerococcus viridans

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
sn-glycerol-3-phosphate + O2 Aerococcus viridans
-
glycerone phosphate + H2O2 i.e. dihydroxyacetone phosphate ?
sn-glycerol-3-phosphate + O2 Aerococcus viridans CETC 978
-
glycerone phosphate + H2O2 i.e. dihydroxyacetone phosphate ?

Organism

Organism UniProt Comment Textmining
Aerococcus viridans
-
-
-
Aerococcus viridans CETC 978
-
-
-

Purification (Commentary)

Purification (Comment) Organism
strain CETC 978, Triton X-114, ammonium sulfate, ion-exchange chromatography, hydrophobic chromatography Aerococcus viridans

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
433.2
-
strain CETC 978 Aerococcus viridans

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
sn-glycerol 3-phosphate + O2
-
Aerococcus viridans glycerone phosphate + H2O2
-
r
sn-glycerol 3-phosphate + O2
-
Aerococcus viridans CETC 978 glycerone phosphate + H2O2
-
r
sn-glycerol-3-phosphate + O2
-
Aerococcus viridans glycerone phosphate + H2O2 i.e. dihydroxyacetone phosphate ?
sn-glycerol-3-phosphate + O2
-
Aerococcus viridans CETC 978 glycerone phosphate + H2O2 i.e. dihydroxyacetone phosphate ?

Subunits

Subunits Comment Organism
? strain CETC 978, x * 63000, SDS-PAGE Aerococcus viridans

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8
-
strain CETC 978 Aerococcus viridans

Cofactor

Cofactor Comment Organism Structure
FAD
-
Aerococcus viridans