Cloned (Comment) | Organism |
---|---|
full length cDNA and C-terminal deleted protein overexpressed in Escherichia coli BL21(DE3), pET-28a and pGEM-T easy plasmids | Lotus japonicus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.019 | - |
1,16-hexadecanediol | 1 mM, purified active LjFAO1 protein, compared to substrate preferences with the AtFAO3 gene of Arabidopsis thaliana | Lotus japonicus | |
0.049 | - |
1-hexadecanol | 1 mM, purified active LjFAO1 protein, compared to substrate preferences with the AtFAO3 gene of Arabidopsis thaliana | Lotus japonicus | |
0.059 | - |
1-Dodecanol | 5 mM, purified active LjFAO1 protein, compared to substrate preferences with the AtFAO3 gene of Arabidopsis thaliana | Lotus japonicus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
long-chain alcohol + O2 | Lotus japonicus | - |
? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Lotus japonicus | - |
ecotype Gifu B-129 | - |
Purification (Comment) | Organism |
---|---|
full length version of LjFAO1 protein, gel purification | Lotus japonicus |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
seedling | 8-day-old seedlings, RT-PCR, whole plant expression shown, cold stress treatment of | Lotus japonicus | - |
silique | lowest mRNA expression in | Lotus japonicus | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
LjFAO1 gene identified, genomic structure and mRNA expression, cold stress profiling of LjFAO1 transcripts, overexpression of full-lenght and C-truncated cDNAs, full-length version but not C-truncated part of overexpressed LjFAO1-gene shows long-chain fatty alcohol oxidase activity, substrate specificites of purified protein shown | Lotus japonicus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
1,16-hexadecanediol + O2 | purified active LjFAO1 protein | Lotus japonicus | ? | - |
? | |
1-dodecanol + O2 | purified active LjFAO1 protein | Lotus japonicus | dodecanal + H2O2 | - |
? | |
1-hexadecanol + O2 | purified active LjFAO1 protein | Lotus japonicus | hexadecanal + H2O2 | - |
? | |
long-chain alcohol + O2 | - |
Lotus japonicus | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
LjFAO1 protein | - |
Lotus japonicus |