Cloned (Comment) | Organism |
---|---|
mutant enzyme S101A is expressed in Escherichia coli Rosetta(DE3)pLysS cells | Arthrobacter globiformis |
Crystallization (Comment) | Organism |
---|---|
mutant enzyme S101A, hanging drop vapor diffusion method, using 80 mM sodium cacodylate, 20% (v/v) PEG6000, 20% (v/v) glycerol, 150 mM Mg-acetate at pH 6.0 | Arthrobacter globiformis |
Protein Variants | Comment | Organism |
---|---|---|
S101A | mutant enzyme with increased efficiencies in the oxidative half-reactions and decreased efficiencies in the reductive half-reactions accompanied by a significant decrease in the overall rate of turnover with choline. Ser101 is important, but not essential, for catalysis | Arthrobacter globiformis |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.25 | - |
choline | wild type enzyme, in 50 mM sodium diphosphate, pH 10.0, at 25°C | Arthrobacter globiformis | |
0.26 | - |
choline | mutant enzyme S101A, in 50 mM sodium diphosphate, pH 10.0, at 25°C | Arthrobacter globiformis |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Arthrobacter globiformis | Q7X2H8 | - |
- |
Purification (Comment) | Organism |
---|---|
- |
Arthrobacter globiformis |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
choline + O2 | - |
Arthrobacter globiformis | betaine aldehyde + H2O2 | - |
r |
Synonyms | Comment | Organism |
---|---|---|
choline-oxygen 1-oxidoreductase | - |
Arthrobacter globiformis |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
6.7 | - |
choline | mutant enzyme S101A, in 50 mM sodium diphosphate, pH 10.0, at 25°C | Arthrobacter globiformis | |
43 | - |
Betaine aldehyde | mutant enzyme S101A, in 50 mM sodium diphosphate, pH 10.0, at 25°C | Arthrobacter globiformis | |
60 | - |
choline | wild type enzyme, in 50 mM sodium diphosphate, pH 10.0, at 25°C | Arthrobacter globiformis | |
133 | - |
Betaine aldehyde | wild type enzyme, in 50 mM sodium diphosphate, pH 10.0, at 25°C | Arthrobacter globiformis |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
FAD | - |
Arthrobacter globiformis |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
25.6 | - |
choline | mutant enzyme S101A, in 50 mM sodium diphosphate, pH 10.0, at 25°C | Arthrobacter globiformis | |
237 | - |
choline | wild type enzyme, in 50 mM sodium diphosphate, pH 10.0, at 25°C | Arthrobacter globiformis |