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Literature summary for 1.1.3.15 extracted from

  • Vignaud, C.; Pietrancosta, N.; Williams, E.L.; Rumsby, G.; Lederer, F.
    Purification and characterization of recombinant human liver glycolate oxidase (2007), Arch. Biochem. Biophys., 465, 410-416.
    View publication on PubMed
Search for more literature for 1.1.3.15

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli as a His-tagged fusion protein Homo sapiens

Inhibitors

Inhibitors Comment Organism Structure
2,6-dichlorophenolindophenol inhibition by excess substrate Homo sapiens
glycolate inhibition by excess substrate Homo sapiens
glyoxylate inhibition by excess substrate Homo sapiens
L-Mandelate inhibition by excess substrate Homo sapiens
Mandelate
-
 Homo sapiens
oxalate
-
 Homo sapiens

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0056
-
 glycolate
-
 Homo sapiens
0.033
-
 2,6-dichlorophenolindophenol
-
 Homo sapiens
0.32
-
 glycolate
-
 Homo sapiens
1.5
-
 L-Mandelate
-
 Homo sapiens
3.4
-
 glyoxylate
-
 Homo sapiens
16.5
-
 L-lactate
-
 Homo sapiens

Localization

Localization Comment Organism GeneOntology No. Textmining

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
41290
-
 determined by mass spectrometry. After tag-removal two close bands appear on SDS-PAGE one at 45.287 kDa and one at 41.288 kDa Homo sapiens
45290
-
 determined by mass spectrometry. After tag-removal two close bands appear on SDS-PAGE one at 45.287 kDa and one at 41.288 kDa Homo sapiens

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
-
-

Purification (Commentary)

Purification (Comment) Organism
purified in one step on hydroxyapatite column onto which it is strongly adsorbed. For the initial purification steps, FMN is added to the buffers Homo sapiens

Source Tissue

Source Tissue Comment Organism Textmining
liver
-
 Homo sapiens
-

Storage Stability

Storage Stability Organism
-70°C, concentrated solution, stable for several months Homo sapiens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
glycolate + 2,6-dichlorophenolindophenol
-
 Homo sapiens glyoxylate + reduced 2,6-dichlorophenolindophenol
-
 ?
glyoxylate + 2,6-dichlorophenolindophenol
-
 Homo sapiens ?
-
 ?
L-lactate + 2,6-dichlorophenolindophenol
-
 Homo sapiens pyruvate + reduced 2,6-dichlorophenolindophenol
-
 ?
L-mandelate + 2,6-dichlorophenolindophenol
-
 Homo sapiens ?
-
 ?

Subunits

Subunits Comment Organism
tetramer gel filtration Homo sapiens

Synonyms

Synonyms Comment Organism
glycolate oxidase
-
 Homo sapiens

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
30
-
 assay at Homo sapiens

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.11
-
 L-Mandelate
-
 Homo sapiens
0.46
-
 L-lactate
-
 Homo sapiens
0.83
-
 glyoxylate
-
 Homo sapiens
3.6
-
 glycolate
-
 Homo sapiens
15.6
-
 glycolate
-
 Homo sapiens

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7
-
 assay at Homo sapiens

Cofactor

Cofactor Comment Organism Structure
FMN
-
 Homo sapiens

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
0.078
-
 2,6-dichlorophenolindophenol
-
 Homo sapiens
12
-
 glyoxylate
-
 Homo sapiens
36
-
 glycolate
-
 Homo sapiens
46
-
 glycolate
-
 Homo sapiens
92
-
 L-Mandelate
-
 Homo sapiens

IC50 Value

IC50 Value IC50 Value Maximum Comment Organism Inhibitor Structure
1.8
-
-
 Homo sapiens oxalate
3.7
-
-
 Homo sapiens Mandelate