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Literature summary for 1.1.3.15 extracted from

  • Cromartie, T.H.; Walsh, C.
    Mechanistic studies on the rat kidney flavoenzyme L-alpha-hydroxy acid oxidase (1975), Biochemistry, 14, 3482-3490.
    View publication on PubMed

Inhibitors

Inhibitors Comment Organism Structure
DL-2-hydroxy-3-butynoate irreversible inactivation after 25 turnovers, covalent addition to the coenzyme Rattus sp.
DL-2-hydroxy-3-heptynoate inactivation after18000 turnovers Rattus sp.
DL-2-hydroxy-3-hexynoate inactivation after 8500 turnovers Rattus sp.
DL-2-hydroxy-3-octynoate inactivation after 15000 turnovers Rattus sp.
DL-2-hydroxy-3-pentynoate inactivation after 4800 turnovers Rattus sp.
DL-vinylglycolate slight inactivation after 10000 turnovers Rattus sp.

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.14
-
DL-2-hydroxy-3-octynoate
-
Rattus sp.
0.38
-
DL-2-hydroxy-3-heptynoate
-
Rattus sp.
4
-
DL-2-hydroxy-3-butynoate
-
Rattus sp.
7
-
DL-2-hydroxy-3-hexynoate
-
Rattus sp.
9
-
DL-2-hydroxy-3-pentynoate
-
Rattus sp.
10
-
DL-vinylglycolate
-
Rattus sp.

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
DL-2-hydroxybutyrate + O2 Rattus sp.
-
2-oxobutyrate + H2O2
-
?

Organism

Organism UniProt Comment Textmining
Rattus sp.
-
-
-

Purification (Commentary)

Purification (Comment) Organism
partial Rattus sp.

Source Tissue

Source Tissue Comment Organism Textmining
kidney
-
Rattus sp.
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
DL-2-hydroxy-3-butynoate + O2 good substrate, but inactivation after 25 turnovers Rattus sp. 2-oxo-3-butynoate + H2O2
-
?
DL-2-hydroxy-3-heptynoate + O2 86% of the activity compared to DL-2-hydroxybutyrate Rattus sp. 2-oxo-3-heptynoate + H2O2
-
?
DL-2-hydroxy-3-hexynoate + O2 65% of the activity compared to DL-2-hydroxybutyrate Rattus sp. 2-oxo-3-hexynoate + H2O2
-
?
DL-2-hydroxy-3-octynoate + O2 70% of the activity compared to DL-2-hydroxybutyrate Rattus sp. 2-oxo-3-octynoate + H2O2
-
?
DL-2-hydroxy-3-pentynoate + O2 2fold higher activity compared to DL-2-hydroxybutyrate Rattus sp. 2-oxo-3-pentynoate + H2O2
-
?
DL-2-hydroxybutyrate + O2
-
Rattus sp. 2-oxobutyrate + H2O2
-
?
DL-3-chlorolactate + O2
-
Rattus sp. 3-chloropyruvate + H2O2
-
?
DL-vinylglycolate + O2 90% of the activity compared to DL-2-hydroxybutyrate Rattus sp. 2-oxo-3-butenoic acid + H2O2
-
?

Cofactor

Cofactor Comment Organism Structure
FMN
-
Rattus sp.