Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 1.1.3.13 extracted from

  • Dmytruk, K.V.; Smutok, O.V.; Ryabova, O.B.; Gayda, G.Z.; Sibirny, V.A.; Schuhmann, W.; Gonchar, M.V.; Sibirny, A.A.
    Isolation and characterization of mutated alcohol oxidases from the yeast Hansenula polymorpha with decreased affinity toward substrates and their use as selective elements of an amperometric biosensor (2007), BMC Biotechnol., 7, 33.
    View publication on PubMedView publication on EuropePMC

Application

Application Comment Organism
analysis selection procedure for isolation of the mutant forms of AOX, and their use as a suitable bioelement for biosensor technologies. The created biosensor based on mAOX (from the strain CA2) was characterised by a decreased affinity towards analyzed substrates and slightly increased Vmax. The operational stability of the mAOX-immobilised electrode is not affected and remains similar to an electrode based on the natural enzyme. The described methodology opens up the possibility for construction of biosensors appropriate for precise, rapid, and cheap analysis of target analytes, e.g. ethanol in real samples of wines, beers or fermentation cultures Ogataea angusta

Organism

Organism UniProt Comment Textmining
Ogataea angusta
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
Ogataea angusta

Synonyms

Synonyms Comment Organism
AOX
-
Ogataea angusta