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Literature summary for 1.1.3.13 extracted from

  • Daniel, G.; Volc, J.; Filonova, L.; Plihal, O.; Kubatova, E.; Halada, P.
    Characteristics of Gloeophyllum trabeum alcohol oxidase, an extracellular source of H2O2 in brown rot decay of wood (2007), Appl. Environ. Microbiol., 73, 6241-6253.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

Cloned (Comment) Organism
-
Gloeophyllum trabeum

General Stability

General Stability Organism
rapid freezing at -80°C and thawing has no significant effect on the enzyme’s activity Gloeophyllum trabeum

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.3
-
methanol
-
Gloeophyllum trabeum

Localization

Localization Comment Organism GeneOntology No. Textmining
cell wall the enzyme is localized in the hyphal periplasmic space and wall and on extracellular tripartite membranes and slime, while there is no labeling of hyphal peroxisomes. AOX is associated with membranous or slime structures secreted by hyphae in wood fiber lumina and within the secondary cell walls of degraded wood fibers Gloeophyllum trabeum 5618
-
extracellular the enzyme is localized in the hyphal periplasmic space and wall and on extracellular tripartite membranes and slime, while there is no labeling of hyphal peroxisomes. AOX is associated with membranous or slime structures secreted by hyphae in wood fiber lumina and within the secondary cell walls of degraded wood fibers Gloeophyllum trabeum
-
-
periplasmic space the enzyme is localized in the hyphal periplasmic space and wall and on extracellular tripartite membranes and slime, while there is no labeling of hyphal peroxisomes. AOX is associated with membranous or slime structures secreted by hyphae in wood fiber lumina and within the secondary cell walls of degraded wood fibers Gloeophyllum trabeum 42597
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
72400
-
8 * 72400, SDS-PAGE Gloeophyllum trabeum
628000
-
equilibrium ultracentrifugation Gloeophyllum trabeum

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
methanol + O2 Gloeophyllum trabeum possible role for AOX as a major source of H2O2 formaldehyde + H2O2
-
?

Organism

Organism UniProt Comment Textmining
Gloeophyllum trabeum A8DPS4
-
-

Posttranslational Modification

Posttranslational Modification Comment Organism
no glycoprotein
-
Gloeophyllum trabeum

Purification (Commentary)

Purification (Comment) Organism
-
Gloeophyllum trabeum

Source Tissue

Source Tissue Comment Organism Textmining
hypha the enzyme is localized in the hyphal periplasmic space and wall and on extracellular tripartite membranes and slime, while there is no labeling of hyphal peroxisomes. AOX is associated with membranous or slime structures secreted by hyphae in wood fiber lumina and within the secondary cell walls of degraded wood fibers Gloeophyllum trabeum
-
mycelium
-
Gloeophyllum trabeum
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
12
-
-
Gloeophyllum trabeum

Storage Stability

Storage Stability Organism
4°C, 1 week storage of the sterile-filtered purified enzyme in 20 mM sodium phosphate (pH 7.5) results in a 68% loss of activity Gloeophyllum trabeum

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1-butanol + O2 54% of the activity with methanol Gloeophyllum trabeum n-butanal + H2O2
-
?
1-pentanol + O2 22% of the activity with methanol Gloeophyllum trabeum n-pentanal + H2O2
-
?
1-propanol + O2 73% of the activity with methanol Gloeophyllum trabeum n-propanal + H2O2
-
?
2-methyl-1-propanol + O2 7% of the activity with methanol Gloeophyllum trabeum butan-2-one + H2O2
-
?
3-methyl-1-butanol + O2 7% of the activity with methanol Gloeophyllum trabeum 3-methylbutanal + H2O2
-
?
allylalcohol + O2 93% of the activity with methanol Gloeophyllum trabeum acrolein + H2O2
-
?
benzyl alcohol + O2 9% of the activity with methanol Gloeophyllum trabeum benzaldehyde + H2O2
-
?
ethanol + O2 94% of the activity with methanol Gloeophyllum trabeum acetaldehyde + H2O2
-
?
methanol + O2
-
Gloeophyllum trabeum formaldehyde + H2O2
-
?
methanol + O2 possible role for AOX as a major source of H2O2 Gloeophyllum trabeum formaldehyde + H2O2
-
?

Subunits

Subunits Comment Organism
octamer 8 * 72400, SDS-PAGE Gloeophyllum trabeum

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
15.6
-
methanol
-
Gloeophyllum trabeum

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6 10 broad optimum Gloeophyllum trabeum

pH Range

pH Minimum pH Maximum Comment Organism
additional information
-
no activity detectable at pH 5.0 and at pH 12.0 Gloeophyllum trabeum
5.5
-
16% of maximal activity Gloeophyllum trabeum

pH Stability

pH Stability pH Stability Maximum Comment Organism
7 11 pH stability optimum on the alkaline side in the range of pH 7 to 11. 80% of maximum stability at pH 9 Gloeophyllum trabeum

Cofactor

Cofactor Comment Organism Structure
FAD noncovalently bonded Gloeophyllum trabeum

pI Value

Organism Comment pI Value Maximum pI Value
Gloeophyllum trabeum isoelectric focusing
-
5.3