Cloned (Comment) | Organism |
---|---|
- |
Gloeophyllum trabeum |
General Stability | Organism |
---|---|
rapid freezing at -80°C and thawing has no significant effect on the enzymes activity | Gloeophyllum trabeum |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
2.3 | - |
methanol | - |
Gloeophyllum trabeum |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
cell wall | the enzyme is localized in the hyphal periplasmic space and wall and on extracellular tripartite membranes and slime, while there is no labeling of hyphal peroxisomes. AOX is associated with membranous or slime structures secreted by hyphae in wood fiber lumina and within the secondary cell walls of degraded wood fibers | Gloeophyllum trabeum | 5618 | - |
extracellular | the enzyme is localized in the hyphal periplasmic space and wall and on extracellular tripartite membranes and slime, while there is no labeling of hyphal peroxisomes. AOX is associated with membranous or slime structures secreted by hyphae in wood fiber lumina and within the secondary cell walls of degraded wood fibers | Gloeophyllum trabeum | - |
- |
periplasmic space | the enzyme is localized in the hyphal periplasmic space and wall and on extracellular tripartite membranes and slime, while there is no labeling of hyphal peroxisomes. AOX is associated with membranous or slime structures secreted by hyphae in wood fiber lumina and within the secondary cell walls of degraded wood fibers | Gloeophyllum trabeum | 42597 | - |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
72400 | - |
8 * 72400, SDS-PAGE | Gloeophyllum trabeum |
628000 | - |
equilibrium ultracentrifugation | Gloeophyllum trabeum |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
methanol + O2 | Gloeophyllum trabeum | possible role for AOX as a major source of H2O2 | formaldehyde + H2O2 | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Gloeophyllum trabeum | A8DPS4 | - |
- |
Posttranslational Modification | Comment | Organism |
---|---|---|
no glycoprotein | - |
Gloeophyllum trabeum |
Purification (Comment) | Organism |
---|---|
- |
Gloeophyllum trabeum |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
hypha | the enzyme is localized in the hyphal periplasmic space and wall and on extracellular tripartite membranes and slime, while there is no labeling of hyphal peroxisomes. AOX is associated with membranous or slime structures secreted by hyphae in wood fiber lumina and within the secondary cell walls of degraded wood fibers | Gloeophyllum trabeum | - |
mycelium | - |
Gloeophyllum trabeum | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
12 | - |
- |
Gloeophyllum trabeum |
Storage Stability | Organism |
---|---|
4°C, 1 week storage of the sterile-filtered purified enzyme in 20 mM sodium phosphate (pH 7.5) results in a 68% loss of activity | Gloeophyllum trabeum |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
1-butanol + O2 | 54% of the activity with methanol | Gloeophyllum trabeum | n-butanal + H2O2 | - |
? | |
1-pentanol + O2 | 22% of the activity with methanol | Gloeophyllum trabeum | n-pentanal + H2O2 | - |
? | |
1-propanol + O2 | 73% of the activity with methanol | Gloeophyllum trabeum | n-propanal + H2O2 | - |
? | |
2-methyl-1-propanol + O2 | 7% of the activity with methanol | Gloeophyllum trabeum | butan-2-one + H2O2 | - |
? | |
3-methyl-1-butanol + O2 | 7% of the activity with methanol | Gloeophyllum trabeum | 3-methylbutanal + H2O2 | - |
? | |
allylalcohol + O2 | 93% of the activity with methanol | Gloeophyllum trabeum | acrolein + H2O2 | - |
? | |
benzyl alcohol + O2 | 9% of the activity with methanol | Gloeophyllum trabeum | benzaldehyde + H2O2 | - |
? | |
ethanol + O2 | 94% of the activity with methanol | Gloeophyllum trabeum | acetaldehyde + H2O2 | - |
? | |
methanol + O2 | - |
Gloeophyllum trabeum | formaldehyde + H2O2 | - |
? | |
methanol + O2 | possible role for AOX as a major source of H2O2 | Gloeophyllum trabeum | formaldehyde + H2O2 | - |
? |
Subunits | Comment | Organism |
---|---|---|
octamer | 8 * 72400, SDS-PAGE | Gloeophyllum trabeum |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
15.6 | - |
methanol | - |
Gloeophyllum trabeum |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6 | 10 | broad optimum | Gloeophyllum trabeum |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
additional information | - |
no activity detectable at pH 5.0 and at pH 12.0 | Gloeophyllum trabeum |
5.5 | - |
16% of maximal activity | Gloeophyllum trabeum |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
7 | 11 | pH stability optimum on the alkaline side in the range of pH 7 to 11. 80% of maximum stability at pH 9 | Gloeophyllum trabeum |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
FAD | noncovalently bonded | Gloeophyllum trabeum |
Organism | Comment | pI Value Maximum | pI Value |
---|---|---|---|
Gloeophyllum trabeum | isoelectric focusing | - |
5.3 |