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Literature summary for 1.1.3.13 extracted from
- Purification and characterization of alcohol oxidase from a genetically constructed over-producing strain of the methylotrophic yeast Hansenula polymorpha (2006), Biochemistry (Moscow), 71, 245-250.
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| NaN3 | competitive, reversible | Ogataea angusta |  |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.4 | - |
methanol | pH 7.5, 30°C | Ogataea angusta | |
| 6.6 | - |
ethanol | pH 7.5, 30°C | Ogataea angusta | |
| 10.5 | - |
formaldehyde | pH 7.5, 30°C | Ogataea angusta | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 80000 | - |
8 * 80000, SDS-PAGE | Ogataea angusta |
| 620000 | - |
gel filtration | Ogataea angusta |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ethanol + O2 | Ogataea angusta | - |
acetaldehyde + H2O2 | - |
? | |
| formaldehyde + O2 | Ogataea angusta | - |
formic acid + H2O2 | - |
? | |
| methanol + O2 | Ogataea angusta | - |
formaldehyde + H2O2 | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Ogataea angusta | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| recombinant enzyme 7.7fold from overproducing Hansenula polymorpha strain C-105 by ammonium sulfate fractionation and ion exchange chromatography to homogeneity | Ogataea angusta |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 23 | - |
purified enzyme | Ogataea angusta |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ethanol + O2 | - |
Ogataea angusta | acetaldehyde + H2O2 | - |
? | |
| formaldehyde + O2 | - |
Ogataea angusta | formic acid + H2O2 | - |
? | |
| formaldehyde + O2 | low activity | Ogataea angusta | formic acid + H2O2 | - |
? | |
| methanol + O2 | - |
Ogataea angusta | formaldehyde + H2O2 | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| More | secondary structure analysis, overview | Ogataea angusta |
| octamer | 8 * 80000, SDS-PAGE | Ogataea angusta |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| AOX | - |
Ogataea angusta |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 30 | - |
assay at | Ogataea angusta |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 32 | - |
formaldehyde | pH 7.5, 30°C | Ogataea angusta | |
| 58 | - |
ethanol | pH 7.5, 30°C | Ogataea angusta | |
| 60 | - |
methanol | pH 7.5, 30°C | Ogataea angusta | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.5 | - |
assay at | Ogataea angusta |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| FAD | - |
Ogataea angusta | |
pI Value
| Organism | Comment | pI Value Maximum | pI Value |
|---|---|---|---|
| Ogataea angusta | isoelectric focusing | - |
6.1 |
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Release 2023.1 (January 2023)
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