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Literature summary for 1.1.3.13 extracted from

  • Sahm, H.; Schuette, H.; Kula, M.R.
    Alcohol oxidase from Candida boidinii (1982), Methods Enzymol., 89, 424-428.
No PubMed abstract available

Inhibitors

Inhibitors Comment Organism Structure
Cu2+ 1 mM, 95% inhibition [Candida] boidinii
PCMB 1 mM, complete inhibition [Candida] boidinii

Localization

Localization Comment Organism GeneOntology No. Textmining
microbody
-
[Candida] boidinii 42579
-

Metals/Ions

Metals/Ions Comment Organism Structure
additional information no metal requirement [Candida] boidinii

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
74000
-
8 * 74000, SDS-PAGE [Candida] boidinii
600000
-
equilibrium sedimentation [Candida] boidinii

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
methanol + O2 [Candida] boidinii key enzyme of methanol metabolism formaldehyde + H2O2
-
?

Organism

Organism UniProt Comment Textmining
[Candida] boidinii
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
[Candida] boidinii

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
10.94
-
-
[Candida] boidinii

Storage Stability

Storage Stability Organism
-20°C, stable [Candida] boidinii

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2-buten-1-ol + O2
-
[Candida] boidinii 2-buten-1-al + H2O2
-
?
2-propen-1-ol + O2
-
[Candida] boidinii 2-propen-1-al + H2O2
-
?
chloroethanol + O2
-
[Candida] boidinii chloroethanal + H2O2
-
?
ethanol + O2
-
[Candida] boidinii acetaldehyde + H2O2
-
?
formaldehyde + O2
-
[Candida] boidinii formate + H2O2
-
?
methanol + O2
-
[Candida] boidinii formaldehyde + H2O2
-
?
methanol + O2 key enzyme of methanol metabolism [Candida] boidinii formaldehyde + H2O2
-
?
n-butanol + O2
-
[Candida] boidinii n-butanal + H2O2
-
?
n-propanol + O2
-
[Candida] boidinii n-propanal + H2O2
-
?

Subunits

Subunits Comment Organism
octamer 8 * 74000, SDS-PAGE [Candida] boidinii

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
30
-
-
[Candida] boidinii

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5 9.5
-
[Candida] boidinii

pH Stability

pH Stability pH Stability Maximum Comment Organism
7 10 stable [Candida] boidinii

Cofactor

Cofactor Comment Organism Structure
FAD 1 mol FAD per mol of subunit [Candida] boidinii