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Literature summary for 1.1.3.12 extracted from
- The bacterial oxidation of vitamin B6. V. The enzymatic formation of pyridoxal and isopyridoxal from pyridoxine (1969), J. Biol. Chem., 244, 2577-2584.
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| 1,10-phenanthroline | - |
Pseudomonas sp. |  |
| 3-chloromercuribenzoate | o-chloromercuribenzoate, m-chloromercuribenzoate, and p-chloromercuribenzoate inhibit in absence but not in presence of cyanide | Pseudomonas sp. | |
| alpha,alpha'-dipyridyl | - |
Pseudomonas sp. | |
| o-chloromercuribenzoate | o-chloromercuribenzoate, m-chloromercuribenzoate, and p-chloromercuribenzoate inhibit in absence but not in presence of cyanide | Pseudomonas sp. | |
| p-chloromercuribenzoate | o-chloromercuribenzoate, m-chloromercuribenzoate, and p-chloromercuribenzoate inhibit in absence but not in presence of cyanide | Pseudomonas sp. | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.0083 | - |
2,6-dichloroindophenol | - |
Pseudomonas sp. | |
| 0.43 | - |
pyridoxine | - |
Pseudomonas sp. | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Pseudomonas sp. | - |
MA-1 | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Pseudomonas sp. |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| additional information | - |
- |
Pseudomonas sp. |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 2-demethyl-2-ethylpyridoxine + O2 | - |
Pseudomonas sp. | 2-demethyl-2-ethylpyridoxal + H2O2 | - |
? | |
| 5-deoxypyridoxine + O2 | - |
Pseudomonas sp. | 5-deoxypyridoxal + H2O2 | - |
? | |
| pyridoxine + 2,6-dichloroindophenol | high degree of specificity for pyridoxine | Pseudomonas sp. | pyridoxol + reduced 2,6-dichlorophenolindophenol | - |
? | |
| pyridoxine + O2 | other acceptor: 2,6-dichloroindophenol, high degree of specificity for pyridoxine | Pseudomonas sp. | pyridoxal + H2O2 | - |
? | |
| pyridoxine + O2 | other acceptor: 2,6-dichloroindophenol, high degree of specificity for pyridoxine | Pseudomonas sp. IA | pyridoxal + H2O2 | - |
? | |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 26 | - |
assay at | Pseudomonas sp. |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.5 | 8 | - |
Pseudomonas sp. |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| additional information | - |
- |
Pseudomonas sp. |
| 7 | 9 | pH 7: 80% of maximal activity, pH 9: 50% of maximal acitvity | Pseudomonas sp. |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| FAD | - |
Pseudomonas sp. | |
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Release 2023.1 (January 2023)
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