Crystallization (Comment) | Organism |
---|---|
P2O H167A mutant in complex with 3-deoxy-3-fluoro-D-glucose, microseeded hanging drops, mixing of equal volumes of 0.1 M MES, pH 5.2, 50 mM MgCl2, 10% w/v PEG monomethyl ether 2000 with 20 mg/ml protein in 20 mM MES, pH 5.2, addition of ligand in saturated mother liquor with 28% w/v PEG 2000, X-ray diffraction structure determination and analysis at 1.35 A resolution | Trametes ochracea |
Protein Variants | Comment | Organism |
---|---|---|
D452A/R472A | the mutant shows drastic effects on the binding constant for D-glucose | Trametes ochracea |
H167A | the P2O mutant in complex with the slow substrate 2-deoxy-2-fluoro-D-glucose shows a substrate-binding mode compatible with oxidation at C3. To accommodate the sugar, a gating segment, 454FSY456, in the substrate recognition loop partly unfolded to create a spacious and more polar active site that is distinct from the closed state of P2O. With 3-deoxy-3-fluoro-D-glucose the mutant prefers C2 oxidation, crystal structure of the ordered complex of P2O H167A with at 1.35 A resolution, overview | Trametes ochracea |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
periplasm | hyphal periplasmic space | Trametes ochracea | - |
- |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
270000 | - |
- |
Trametes ochracea |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
D-glucose + O2 | Trametes ochracea | highly regioselective mechanism | 2-dehydro-D-glucose + H2O2 | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Trametes ochracea | Q7ZA32 | - |
- |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
D-glucose + O2 = 2-dehydro-D-glucose + H2O2 | highly regioselective mechanism, overview. The different conformations of the 454FSY456 gating segment in the semiopen and closed states induce backbone and side-chain movements of Thr169 and Asp452 that add further differential stabilization to the individual states | Trametes ochracea |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
D-glucose + O2 | highly regioselective mechanism | Trametes ochracea | 2-dehydro-D-glucose + H2O2 | - |
? |
Subunits | Comment | Organism |
---|---|---|
homotetramer | - |
Trametes ochracea |
Synonyms | Comment | Organism |
---|---|---|
P2O | - |
Trametes ochracea |
pyranose 2-Oxidase | - |
Trametes ochracea |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
FAD | dependent on | Trametes ochracea |
General Information | Comment | Organism |
---|---|---|
additional information | substrate recognition loop, active-site protein interactions for D-glucose, and structural determinants of the productive glucose C2-oxidation mode, detailed overview | Trametes ochracea |