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Literature summary for 1.1.3.10 extracted from

  • Koths, K.; Halenbeck, R.; Moreland, M.
    Synthesis of the antibiotic cortalcerone from D-glucose using pyranose 2-oxidase and a novel fungal enzyme, aldos-2-ulose dehydratase (1992), Carbohydr. Res., 232, 59-75.
    View publication on PubMed

Organism

Organism UniProt Comment Textmining
Polyporus obtusus
-
-
-
Polyporus obtusus AU124PD
-
-
-

Purification (Commentary)

Purification (Comment) Organism
partial purification, PEG 4000 precipitation, DEAE-Sepharose column chromatography, and gel filtration Polyporus obtusus

Source Tissue

Source Tissue Comment Organism Textmining
mycelium
-
Polyporus obtusus
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
8.5
-
purified enzyme, at pH 5.0 and 25°C Polyporus obtusus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
D-glucose + O2
-
Polyporus obtusus D-arabino-hexos-2-ulose + H2O2 the product is a specific tautomeric form of D-arabino-hexos-2-ulose (form IV, 20%) being in equilibrium with three other forms of D-arabinohexos-2-ulose in solution ?
D-glucose + O2
-
Polyporus obtusus AU124PD D-arabino-hexos-2-ulose + H2O2 the product is a specific tautomeric form of D-arabino-hexos-2-ulose (form IV, 20%) being in equilibrium with three other forms of D-arabinohexos-2-ulose in solution ?
D-xylose + O2
-
Polyporus obtusus D-threo-pentos-2-ulose + H2O2
-
?
D-xylose + O2
-
Polyporus obtusus AU124PD D-threo-pentos-2-ulose + H2O2
-
?

Synonyms

Synonyms Comment Organism
P2O
-
Polyporus obtusus
pyranose 2-Oxidase
-
Polyporus obtusus