Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli BL21 Star (DE3) cells | Trametes ochracea |
Crystallization (Comment) | Organism |
---|---|
mutant enzymes are crystallized by the hanging drop vapor diffusion method, using 10% (w/v) monomethyl ether poly(ethlene glycol) 2000 , 0.1 M MES (pH 5.2), 50 mM MgCl2 | Trametes ochracea |
Protein Variants | Comment | Organism |
---|---|---|
F454A/S455A/Y456A | the mutant shows decreased catalytic efficiency for D-glucose/O2 compared to the wild type enzyme | Trametes ochracea |
F454A/Y456A | the mutant shows decreased catalytic efficiency for D-glucose/O2 compared to the wild type enzyme | Trametes ochracea |
F454N | the apparent kcat value with D-glucose and O2 (fixed at a concentration of 0.256 mM, air saturation) as substrates decreases dramatically compared to the wild type enzyme | Trametes ochracea |
F454N | the mutant shows decreased catalytic efficiency for D-glucose/O2 compared to the wild type enzyme | Trametes ochracea |
F454P | the mutant shows strongly decreased catalytic efficiency for D-glucose/O2 compared to the wild type enzyme | Trametes ochracea |
H450Q | the turnover number for the electron donor/acceptor substrate pair D-galactose/O2 is comparable to that of the wild type enzyme | Trametes ochracea |
additional information | the deletion mutant DELTA454-456 shows 6fold lower kcat (D-galactose/O2), and 0.8fold elevated Km (D-galactose) value compared to the wild type enzyme | Trametes ochracea |
Y456W | the turnover number for the electron donor/acceptor substrate pair D-galactose/O2 is comparable to that of the wild type enzyme | Trametes ochracea |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0052 | - |
1,4-benzoquinone | mutant enzyme F454N, using D-galactose as cosubstrate, in 50 mM KH2PO4 buffer (pH 6.5), at 30°C | Trametes ochracea | |
0.0071 | - |
1,4-benzoquinone | mutant enzyme F454P, using D-galactose as cosubstrate, in 50 mM KH2PO4 buffer (pH 6.5), at 30°C | Trametes ochracea | |
0.0089 | - |
1,4-benzoquinone | mutant enzyme F454A/S455A/Y456A, using D-galactose as cosubstrate, in 50 mM KH2PO4 buffer (pH 6.5), at 30°C | Trametes ochracea | |
0.01 | - |
1,4-benzoquinone | mutant enzyme Y456W, using D-galactose as cosubstrate, in 50 mM KH2PO4 buffer (pH 6.5), at 30°C | Trametes ochracea | |
0.013 | - |
1,4-benzoquinone | mutant enzyme H450Q, using D-galactose as cosubstrate, in 50 mM KH2PO4 buffer (pH 6.5), at 30°C | Trametes ochracea | |
0.015 | - |
ferrocenium hexafluorophosphate | mutant enzyme F454A/S455A/Y456A, using D-galactose as cosubstrate, in 50 mM KH2PO4 buffer (pH 6.5), at 30°C | Trametes ochracea | |
0.016 | - |
ferrocenium hexafluorophosphate | mutant enzyme F454A/Y456A, using D-galactose as cosubstrate, in 50 mM KH2PO4 buffer (pH 6.5), at 30°C | Trametes ochracea | |
0.023 | - |
ferrocenium hexafluorophosphate | mutant enzyme F454P, using D-galactose as cosubstrate, in 50 mM KH2PO4 buffer (pH 6.5), at 30°C | Trametes ochracea | |
0.025 | - |
ferrocenium hexafluorophosphate | mutant enzyme F454N, using D-galactose as cosubstrate, in 50 mM KH2PO4 buffer (pH 6.5), at 30°C | Trametes ochracea | |
0.027 | - |
1,4-benzoquinone | wild type enzyme, using D-galactose as cosubstrate, in 50 mM KH2PO4 buffer (pH 6.5), at 30°C | Trametes ochracea | |
0.029 | - |
1,4-benzoquinone | mutant enzyme F454A/S455A/Y456A, using D-glucose as cosubstrate, in 50 mM KH2PO4 buffer (pH 6.5), at 30°C | Trametes ochracea | |
0.029 | - |
1,4-benzoquinone | mutant enzyme F454A/Y456A, using D-glucose as cosubstrate, in 50 mM KH2PO4 buffer (pH 6.5), at 30°C | Trametes ochracea | |
0.041 | - |
ferrocenium hexafluorophosphate | mutant enzyme Y456W, using D-galactose as cosubstrate, in 50 mM KH2PO4 buffer (pH 6.5), at 30°C | Trametes ochracea | |
0.049 | - |
ferrocenium hexafluorophosphate | mutant enzyme H450Q, using D-galactose as cosubstrate, in 50 mM KH2PO4 buffer (pH 6.5), at 30°C | Trametes ochracea | |
0.052 | - |
1,4-benzoquinone | mutant enzyme F454N, using D-glucose as cosubstrate, in 50 mM KH2PO4 buffer (pH 6.5), at 30°C | Trametes ochracea | |
0.072 | - |
1,4-benzoquinone | mutant enzyme F454P, using D-glucose as cosubstrate, in 50 mM KH2PO4 buffer (pH 6.5), at 30°C | Trametes ochracea | |
0.072 | - |
1,4-benzoquinone | mutant enzyme Y456W, using D-glucose as cosubstrate, in 50 mM KH2PO4 buffer (pH 6.5), at 30°C | Trametes ochracea | |
0.078 | - |
1,4-benzoquinone | mutant enzyme F454A/Y456A, using D-galactose as cosubstrate, in 50 mM KH2PO4 buffer (pH 6.5), at 30°C | Trametes ochracea | |
0.1 | - |
ferrocenium hexafluorophosphate | wild type enzyme, using D-galactose as cosubstrate, in 50 mM KH2PO4 buffer (pH 6.5), at 30°C | Trametes ochracea | |
0.14 | - |
ferrocenium hexafluorophosphate | mutant enzyme F454P, using D-glucose as cosubstrate, in 50 mM KH2PO4 buffer (pH 6.5), at 30°C | Trametes ochracea | |
0.14 | - |
1,4-benzoquinone | wild type enzyme, using D-glucose as cosubstrate, in 50 mM KH2PO4 buffer (pH 6.5), at 30°C | Trametes ochracea | |
0.15 | - |
ferrocenium hexafluorophosphate | mutant enzyme F454A/Y456A, using D-glucose as cosubstrate, in 50 mM KH2PO4 buffer (pH 6.5), at 30°C | Trametes ochracea | |
0.24 | - |
1,4-benzoquinone | mutant enzyme H450Q, using D-glucose as cosubstrate, in 50 mM KH2PO4 buffer (pH 6.5), at 30°C | Trametes ochracea | |
0.24 | - |
ferrocenium hexafluorophosphate | mutant enzyme Y456W, using D-glucose as cosubstrate, in 50 mM KH2PO4 buffer (pH 6.5), at 30°C | Trametes ochracea | |
0.28 | - |
ferrocenium hexafluorophosphate | mutant enzyme F454A/S455A/Y456A, using D-glucose as cosubstrate, in 50 mM KH2PO4 buffer (pH 6.5), at 30°C | Trametes ochracea | |
0.35 | - |
ferrocenium hexafluorophosphate | mutant enzyme F454N, using D-glucose as cosubstrate, in 50 mM KH2PO4 buffer (pH 6.5), at 30°C | Trametes ochracea | |
0.38 | - |
ferrocenium hexafluorophosphate | mutant enzyme H450Q, using D-glucose as cosubstrate, in 50 mM KH2PO4 buffer (pH 6.5), at 30°C | Trametes ochracea | |
0.4 | - |
ferrocenium hexafluorophosphate | wild type enzyme, using D-glucose as cosubstrate, in 50 mM KH2PO4 buffer (pH 6.5), at 30°C | Trametes ochracea | |
0.76 | - |
D-glucose | wild type enzyme, using O2 as cosubstrate, in 50 mM KH2PO4 buffer (pH 6.5), at 30°C | Trametes ochracea | |
1.5 | - |
D-glucose | mutant enzyme F454A/Y456A, using O2 as cosubstrate, in 50 mM KH2PO4 buffer (pH 6.5), at 30°C | Trametes ochracea | |
1.5 | - |
D-glucose | mutant enzyme F454N, using O2 as cosubstrate, in 50 mM KH2PO4 buffer (pH 6.5), at 30°C | Trametes ochracea | |
1.7 | - |
D-glucose | mutant enzyme Y456W, using O2 as cosubstrate, in 50 mM KH2PO4 buffer (pH 6.5), at 30°C | Trametes ochracea | |
1.8 | - |
D-glucose | mutant enzyme F454P, using O2 as cosubstrate, in 50 mM KH2PO4 buffer (pH 6.5), at 30°C | Trametes ochracea | |
2 | 3 | D-melibiose | mutant enzyme F454A/S455A/Y456A, using O2 as cosubstrate, in 50 mM KH2PO4 buffer (pH 6.5), at 30°C | Trametes ochracea | |
2.1 | - |
D-glucose | mutant enzyme F454A/S455A/Y456A, using O2 as cosubstrate, in 50 mM KH2PO4 buffer (pH 6.5), at 30°C | Trametes ochracea | |
2.5 | - |
D-glucose | mutant enzyme H450Q, using O2 as cosubstrate, in 50 mM KH2PO4 buffer (pH 6.5), at 30°C | Trametes ochracea | |
6.1 | - |
D-galactose | wild type enzyme, using O2 as cosubstrate, in 50 mM KH2PO4 buffer (pH 6.5), at 30°C | Trametes ochracea | |
10 | - |
D-galactose | mutant enzyme F454A/Y456A, using O2 as cosubstrate, in 50 mM KH2PO4 buffer (pH 6.5), at 30°C | Trametes ochracea | |
13 | - |
D-galactose | mutant enzyme F454A/S455A/Y456A, using O2 as cosubstrate, in 50 mM KH2PO4 buffer (pH 6.5), at 30°C | Trametes ochracea | |
13 | - |
D-galactose | mutant enzyme F454P, using O2 as cosubstrate, in 50 mM KH2PO4 buffer (pH 6.5), at 30°C | Trametes ochracea | |
26 | - |
D-galactose | mutant enzyme F454N, using O2 as cosubstrate, in 50 mM KH2PO4 buffer (pH 6.5), at 30°C | Trametes ochracea | |
29 | - |
D-galactose | mutant enzyme Y456W, using O2 as cosubstrate, in 50 mM KH2PO4 buffer (pH 6.5), at 30°C | Trametes ochracea | |
34 | - |
D-galactose | mutant enzyme H450Q, using O2 as cosubstrate, in 50 mM KH2PO4 buffer (pH 6.5), at 30°C | Trametes ochracea | |
50 | - |
D-melibiose | mutant enzyme F454P, using O2 as cosubstrate, in 50 mM KH2PO4 buffer (pH 6.5), at 30°C | Trametes ochracea | |
240 | - |
D-melibiose | mutant enzyme F454N, using O2 as cosubstrate, in 50 mM KH2PO4 buffer (pH 6.5), at 30°C | Trametes ochracea | |
260 | - |
D-melibiose | mutant enzyme Y456W, using O2 as cosubstrate, in 50 mM KH2PO4 buffer (pH 6.5), at 30°C | Trametes ochracea | |
350 | - |
D-melibiose | mutant enzyme F454A/Y456A, using O2 as cosubstrate, in 50 mM KH2PO4 buffer (pH 6.5), at 30°C | Trametes ochracea | |
390 | - |
D-melibiose | mutant enzyme H450Q, using O2 as cosubstrate, in 50 mM KH2PO4 buffer (pH 6.5), at 30°C | Trametes ochracea | |
1500 | - |
D-melibiose | wild type enzyme, using O2 as cosubstrate, in 50 mM KH2PO4 buffer (pH 6.5), at 30°C | Trametes ochracea |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
270000 | - |
gel filtration | Trametes ochracea |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Trametes ochracea | Q7ZA32 | synonym Trametes ochracea | - |
Purification (Comment) | Organism |
---|---|
Ni2+-immobilized metal affinity column chromatography | Trametes ochracea |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2-deoxy-2-fluoro-D-glucose + O2 | slow substrate | Trametes ochracea | 2-deoxy-3-dehydro-D-glucose + H2O2 | - |
? | |
beta-D-galactose + O2 | poor substrate | Trametes ochracea | 2-dehydro-D-galactose + H2O2 | - |
? | |
D-galactose + 1,4-benzoquinone | - |
Trametes ochracea | 2-dehydro-D-galactose + hydroquinone | - |
? | |
D-galactose + ferrocenium hexafluorophosphate | - |
Trametes ochracea | 2-dehydro-D-galactose + ferrocene | - |
? | |
D-glucose + 1,4-benzoquinone | 1,4-benzoquinone is a physiologically relevant alternative electron acceptor in the oxidative half-reaction | Trametes ochracea | 2-dehydro-D-glucose + hydroquinone | - |
? | |
D-glucose + ferrocenium hexafluorophosphate | - |
Trametes ochracea | 2-dehydro-D-glucose + ferrocene | - |
? | |
D-glucose + O2 | the wild type enzyme displays a clear preference for D-glucose over D-galactose | Trametes ochracea | 2-dehydro-D-glucose + H2O2 | - |
? | |
D-melibiose + O2 | - |
Trametes ochracea | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
homotetramer | 2 * 000, SDS-PAGE | Trametes ochracea |
Synonyms | Comment | Organism |
---|---|---|
P2O | - |
Trametes ochracea |
pyranose 2-Oxidase | - |
Trametes ochracea |
pyranose:oxygen 2-oxidoreductase | - |
Trametes ochracea |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
60 | 70 | the half life of the wild type enzyme at 60°C and 70°C is 12 min and 0.07 min, respectively. The melting temperature of the wild type enzyme is at 63.5°C | Trametes ochracea |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.14 | - |
D-galactose | mutant enzyme F454A/S455A/Y456A, using O2 as cosubstrate, in 50 mM KH2PO4 buffer (pH 6.5), at 30°C | Trametes ochracea | |
0.19 | - |
D-melibiose | mutant enzyme F454A/S455A/Y456A, using O2 as cosubstrate, in 50 mM KH2PO4 buffer (pH 6.5), at 30°C | Trametes ochracea | |
0.2 | - |
D-glucose | mutant enzyme F454A/S455A/Y456A, using O2 as cosubstrate, in 50 mM KH2PO4 buffer (pH 6.5), at 30°C | Trametes ochracea | |
0.29 | - |
D-melibiose | mutant enzyme F454P, using O2 as cosubstrate, in 50 mM KH2PO4 buffer (pH 6.5), at 30°C | Trametes ochracea | |
0.3 | - |
D-galactose | mutant enzyme F454P, using O2 as cosubstrate, in 50 mM KH2PO4 buffer (pH 6.5), at 30°C | Trametes ochracea | |
0.64 | - |
D-galactose | mutant enzyme F454A/Y456A, using O2 as cosubstrate, in 50 mM KH2PO4 buffer (pH 6.5), at 30°C | Trametes ochracea | |
0.99 | - |
D-glucose | mutant enzyme F454P, using O2 as cosubstrate, in 50 mM KH2PO4 buffer (pH 6.5), at 30°C | Trametes ochracea | |
1.2 | - |
1,4-benzoquinone | mutant enzyme F454A/S455A/Y456A, using D-galactose as cosubstrate, in 50 mM KH2PO4 buffer (pH 6.5), at 30°C | Trametes ochracea | |
1.2 | - |
D-galactose | mutant enzyme F454N, using O2 as cosubstrate, in 50 mM KH2PO4 buffer (pH 6.5), at 30°C | Trametes ochracea | |
1.3 | - |
D-melibiose | mutant enzyme F454A/Y456A, using O2 as cosubstrate, in 50 mM KH2PO4 buffer (pH 6.5), at 30°C | Trametes ochracea | |
1.5 | - |
D-galactose | mutant enzyme Y456W, using O2 as cosubstrate, in 50 mM KH2PO4 buffer (pH 6.5), at 30°C | Trametes ochracea | |
1.7 | - |
D-galactose | wild type enzyme, using O2 as cosubstrate, in 50 mM KH2PO4 buffer (pH 6.5), at 30°C | Trametes ochracea | |
2 | - |
1,4-benzoquinone | mutant enzyme F454P, using D-galactose as cosubstrate, in 50 mM KH2PO4 buffer (pH 6.5), at 30°C | Trametes ochracea | |
2 | - |
D-galactose | mutant enzyme H450Q, using O2 as cosubstrate, in 50 mM KH2PO4 buffer (pH 6.5), at 30°C | Trametes ochracea | |
2.7 | - |
1,4-benzoquinone | mutant enzyme F454N, using D-galactose as cosubstrate, in 50 mM KH2PO4 buffer (pH 6.5), at 30°C | Trametes ochracea | |
2.7 | - |
D-melibiose | mutant enzyme F454N, using O2 as cosubstrate, in 50 mM KH2PO4 buffer (pH 6.5), at 30°C | Trametes ochracea | |
2.9 | - |
1,4-benzoquinone | mutant enzyme F454A/Y456A, using D-galactose as cosubstrate, in 50 mM KH2PO4 buffer (pH 6.5), at 30°C | Trametes ochracea | |
2.9 | - |
ferrocenium hexafluorophosphate | mutant enzyme F454A/Y456A, using D-galactose as cosubstrate, in 50 mM KH2PO4 buffer (pH 6.5), at 30°C | Trametes ochracea | |
3 | - |
1,4-benzoquinone | mutant enzyme H450Q, using D-galactose as cosubstrate, in 50 mM KH2PO4 buffer (pH 6.5), at 30°C | Trametes ochracea | |
3.3 | - |
D-melibiose | mutant enzyme H450Q, using O2 as cosubstrate, in 50 mM KH2PO4 buffer (pH 6.5), at 30°C | Trametes ochracea | |
3.3 | - |
1,4-benzoquinone | mutant enzyme Y456W, using D-galactose as cosubstrate, in 50 mM KH2PO4 buffer (pH 6.5), at 30°C | Trametes ochracea | |
3.8 | - |
1,4-benzoquinone | wild type enzyme, using D-galactose as cosubstrate, in 50 mM KH2PO4 buffer (pH 6.5), at 30°C | Trametes ochracea | |
4.4 | - |
D-melibiose | mutant enzyme Y456W, using O2 as cosubstrate, in 50 mM KH2PO4 buffer (pH 6.5), at 30°C | Trametes ochracea | |
4.5 | - |
ferrocenium hexafluorophosphate | mutant enzyme F454P, using D-galactose as cosubstrate, in 50 mM KH2PO4 buffer (pH 6.5), at 30°C | Trametes ochracea | |
5.4 | - |
ferrocenium hexafluorophosphate | mutant enzyme F454A/S455A/Y456A, using D-galactose as cosubstrate, in 50 mM KH2PO4 buffer (pH 6.5), at 30°C | Trametes ochracea | |
6.6 | - |
ferrocenium hexafluorophosphate | wild type enzyme, using D-galactose as cosubstrate, in 50 mM KH2PO4 buffer (pH 6.5), at 30°C | Trametes ochracea | |
7.1 | - |
D-glucose | mutant enzyme F454A/Y456A, using O2 as cosubstrate, in 50 mM KH2PO4 buffer (pH 6.5), at 30°C | Trametes ochracea | |
7.3 | - |
ferrocenium hexafluorophosphate | mutant enzyme F454N, using D-galactose as cosubstrate, in 50 mM KH2PO4 buffer (pH 6.5), at 30°C | Trametes ochracea | |
7.3 | - |
ferrocenium hexafluorophosphate | mutant enzyme Y456W, using D-galactose as cosubstrate, in 50 mM KH2PO4 buffer (pH 6.5), at 30°C | Trametes ochracea | |
7.6 | - |
D-melibiose | wild type enzyme, using O2 as cosubstrate, in 50 mM KH2PO4 buffer (pH 6.5), at 30°C | Trametes ochracea | |
12 | - |
D-glucose | mutant enzyme F454N, using O2 as cosubstrate, in 50 mM KH2PO4 buffer (pH 6.5), at 30°C | Trametes ochracea | |
15 | - |
1,4-benzoquinone | mutant enzyme F454A/S455A/Y456A, using D-glucose as cosubstrate, in 50 mM KH2PO4 buffer (pH 6.5), at 30°C | Trametes ochracea | |
17 | - |
ferrocenium hexafluorophosphate | mutant enzyme H450Q, using D-galactose as cosubstrate, in 50 mM KH2PO4 buffer (pH 6.5), at 30°C | Trametes ochracea | |
17 | - |
D-glucose | mutant enzyme H450Q, using O2 as cosubstrate, in 50 mM KH2PO4 buffer (pH 6.5), at 30°C | Trametes ochracea | |
26 | - |
D-glucose | mutant enzyme Y456W, using O2 as cosubstrate, in 50 mM KH2PO4 buffer (pH 6.5), at 30°C | Trametes ochracea | |
30 | - |
1,4-benzoquinone | mutant enzyme F454P, using D-glucose as cosubstrate, in 50 mM KH2PO4 buffer (pH 6.5), at 30°C | Trametes ochracea | |
33 | - |
D-glucose | wild type enzyme, using O2 as cosubstrate, in 50 mM KH2PO4 buffer (pH 6.5), at 30°C | Trametes ochracea | |
61 | - |
1,4-benzoquinone | mutant enzyme F454A/Y456A, using D-glucose as cosubstrate, in 50 mM KH2PO4 buffer (pH 6.5), at 30°C | Trametes ochracea | |
67 | - |
ferrocenium hexafluorophosphate | mutant enzyme F454P, using D-glucose as cosubstrate, in 50 mM KH2PO4 buffer (pH 6.5), at 30°C | Trametes ochracea | |
74 | - |
ferrocenium hexafluorophosphate | mutant enzyme F454A/Y456A, using D-glucose as cosubstrate, in 50 mM KH2PO4 buffer (pH 6.5), at 30°C | Trametes ochracea | |
110 | - |
ferrocenium hexafluorophosphate | mutant enzyme F454A/S455A/Y456A, using D-glucose as cosubstrate, in 50 mM KH2PO4 buffer (pH 6.5), at 30°C | Trametes ochracea | |
130 | - |
1,4-benzoquinone | mutant enzyme F454N, using D-glucose as cosubstrate, in 50 mM KH2PO4 buffer (pH 6.5), at 30°C | Trametes ochracea | |
160 | - |
1,4-benzoquinone | wild type enzyme, using D-glucose as cosubstrate, in 50 mM KH2PO4 buffer (pH 6.5), at 30°C | Trametes ochracea | |
210 | - |
ferrocenium hexafluorophosphate | wild type enzyme, using D-glucose as cosubstrate, in 50 mM KH2PO4 buffer (pH 6.5), at 30°C | Trametes ochracea | |
220 | - |
1,4-benzoquinone | mutant enzyme H450Q, using D-glucose as cosubstrate, in 50 mM KH2PO4 buffer (pH 6.5), at 30°C | Trametes ochracea | |
220 | - |
1,4-benzoquinone | mutant enzyme Y456W, using D-glucose as cosubstrate, in 50 mM KH2PO4 buffer (pH 6.5), at 30°C | Trametes ochracea | |
400 | - |
ferrocenium hexafluorophosphate | mutant enzyme Y456W, using D-glucose as cosubstrate, in 50 mM KH2PO4 buffer (pH 6.5), at 30°C | Trametes ochracea | |
420 | - |
ferrocenium hexafluorophosphate | mutant enzyme F454N, using D-glucose as cosubstrate, in 50 mM KH2PO4 buffer (pH 6.5), at 30°C | Trametes ochracea | |
470 | - |
ferrocenium hexafluorophosphate | mutant enzyme H450Q, using D-glucose as cosubstrate, in 50 mM KH2PO4 buffer (pH 6.5), at 30°C | Trametes ochracea |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
FAD | - |
Trametes ochracea |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0037 | - |
D-melibiose | mutant enzyme F454A/Y456A, using O2 as cosubstrate, in 50 mM KH2PO4 buffer (pH 6.5), at 30°C | Trametes ochracea | |
0.005 | - |
D-melibiose | wild type enzyme, using O2 as cosubstrate, in 50 mM KH2PO4 buffer (pH 6.5), at 30°C | Trametes ochracea | |
0.0058 | - |
D-melibiose | mutant enzyme F454P, using O2 as cosubstrate, in 50 mM KH2PO4 buffer (pH 6.5), at 30°C | Trametes ochracea | |
0.008 | - |
D-melibiose | mutant enzyme F454A/S455A/Y456A, using O2 as cosubstrate, in 50 mM KH2PO4 buffer (pH 6.5), at 30°C | Trametes ochracea | |
0.0084 | - |
D-melibiose | mutant enzyme H450Q, using O2 as cosubstrate, in 50 mM KH2PO4 buffer (pH 6.5), at 30°C | Trametes ochracea | |
0.011 | - |
D-galactose | mutant enzyme F454A/S455A/Y456A, using O2 as cosubstrate, in 50 mM KH2PO4 buffer (pH 6.5), at 30°C | Trametes ochracea | |
0.0113 | - |
D-melibiose | mutant enzyme F454N, using O2 as cosubstrate, in 50 mM KH2PO4 buffer (pH 6.5), at 30°C | Trametes ochracea | |
0.0166 | - |
D-melibiose | mutant enzyme Y456W, using O2 as cosubstrate, in 50 mM KH2PO4 buffer (pH 6.5), at 30°C | Trametes ochracea | |
0.024 | - |
D-galactose | mutant enzyme F454P, using O2 as cosubstrate, in 50 mM KH2PO4 buffer (pH 6.5), at 30°C | Trametes ochracea | |
0.046 | - |
D-galactose | mutant enzyme F454N, using O2 as cosubstrate, in 50 mM KH2PO4 buffer (pH 6.5), at 30°C | Trametes ochracea | |
0.053 | - |
D-galactose | mutant enzyme Y456W, using O2 as cosubstrate, in 50 mM KH2PO4 buffer (pH 6.5), at 30°C | Trametes ochracea | |
0.059 | - |
D-galactose | mutant enzyme H450Q, using O2 as cosubstrate, in 50 mM KH2PO4 buffer (pH 6.5), at 30°C | Trametes ochracea | |
0.062 | - |
D-galactose | mutant enzyme F454A/Y456A, using O2 as cosubstrate, in 50 mM KH2PO4 buffer (pH 6.5), at 30°C | Trametes ochracea | |
0.094 | - |
D-glucose | mutant enzyme F454A/S455A/Y456A, using O2 as cosubstrate, in 50 mM KH2PO4 buffer (pH 6.5), at 30°C | Trametes ochracea | |
0.27 | - |
D-galactose | wild type enzyme, using O2 as cosubstrate, in 50 mM KH2PO4 buffer (pH 6.5), at 30°C | Trametes ochracea | |
0.54 | - |
D-glucose | mutant enzyme F454P, using O2 as cosubstrate, in 50 mM KH2PO4 buffer (pH 6.5), at 30°C | Trametes ochracea | |
4.7 | - |
D-glucose | mutant enzyme F454A/Y456A, using O2 as cosubstrate, in 50 mM KH2PO4 buffer (pH 6.5), at 30°C | Trametes ochracea | |
7 | - |
D-glucose | mutant enzyme H450Q, using O2 as cosubstrate, in 50 mM KH2PO4 buffer (pH 6.5), at 30°C | Trametes ochracea | |
8.2 | - |
D-glucose | mutant enzyme F454N, using O2 as cosubstrate, in 50 mM KH2PO4 buffer (pH 6.5), at 30°C | Trametes ochracea | |
15 | - |
D-glucose | mutant enzyme Y456W, using O2 as cosubstrate, in 50 mM KH2PO4 buffer (pH 6.5), at 30°C | Trametes ochracea | |
37 | - |
1,4-benzoquinone | mutant enzyme F454A/Y456A, using D-galactose as cosubstrate, in 50 mM KH2PO4 buffer (pH 6.5), at 30°C | Trametes ochracea | |
43 | - |
D-glucose | wild type enzyme, using O2 as cosubstrate, in 50 mM KH2PO4 buffer (pH 6.5), at 30°C | Trametes ochracea | |
63 | - |
ferrocenium hexafluorophosphate | wild type enzyme, using D-galactose as cosubstrate, in 50 mM KH2PO4 buffer (pH 6.5), at 30°C | Trametes ochracea | |
130 | - |
1,4-benzoquinone | mutant enzyme F454A/S455A/Y456A, using D-galactose as cosubstrate, in 50 mM KH2PO4 buffer (pH 6.5), at 30°C | Trametes ochracea | |
140 | - |
1,4-benzoquinone | wild type enzyme, using D-galactose as cosubstrate, in 50 mM KH2PO4 buffer (pH 6.5), at 30°C | Trametes ochracea | |
180 | - |
ferrocenium hexafluorophosphate | mutant enzyme F454A/Y456A, using D-galactose as cosubstrate, in 50 mM KH2PO4 buffer (pH 6.5), at 30°C | Trametes ochracea | |
180 | - |
ferrocenium hexafluorophosphate | mutant enzyme Y456W, using D-galactose as cosubstrate, in 50 mM KH2PO4 buffer (pH 6.5), at 30°C | Trametes ochracea | |
200 | - |
ferrocenium hexafluorophosphate | mutant enzyme F454P, using D-galactose as cosubstrate, in 50 mM KH2PO4 buffer (pH 6.5), at 30°C | Trametes ochracea | |
230 | - |
1,4-benzoquinone | mutant enzyme H450Q, using D-galactose as cosubstrate, in 50 mM KH2PO4 buffer (pH 6.5), at 30°C | Trametes ochracea | |
280 | - |
1,4-benzoquinone | mutant enzyme F454P, using D-galactose as cosubstrate, in 50 mM KH2PO4 buffer (pH 6.5), at 30°C | Trametes ochracea | |
290 | - |
ferrocenium hexafluorophosphate | mutant enzyme F454N, using D-galactose as cosubstrate, in 50 mM KH2PO4 buffer (pH 6.5), at 30°C | Trametes ochracea | |
330 | - |
1,4-benzoquinone | mutant enzyme Y456W, using D-galactose as cosubstrate, in 50 mM KH2PO4 buffer (pH 6.5), at 30°C | Trametes ochracea | |
340 | - |
ferrocenium hexafluorophosphate | mutant enzyme H450Q, using D-galactose as cosubstrate, in 50 mM KH2PO4 buffer (pH 6.5), at 30°C | Trametes ochracea | |
360 | - |
ferrocenium hexafluorophosphate | mutant enzyme F454A/S455A/Y456A, using D-galactose as cosubstrate, in 50 mM KH2PO4 buffer (pH 6.5), at 30°C | Trametes ochracea | |
370 | - |
ferrocenium hexafluorophosphate | mutant enzyme F454A/S455A/Y456A, using D-glucose as cosubstrate, in 50 mM KH2PO4 buffer (pH 6.5), at 30°C | Trametes ochracea | |
420 | - |
1,4-benzoquinone | mutant enzyme F454P, using D-glucose as cosubstrate, in 50 mM KH2PO4 buffer (pH 6.5), at 30°C | Trametes ochracea | |
480 | - |
ferrocenium hexafluorophosphate | mutant enzyme F454P, using D-glucose as cosubstrate, in 50 mM KH2PO4 buffer (pH 6.5), at 30°C | Trametes ochracea | |
500 | - |
ferrocenium hexafluorophosphate | mutant enzyme F454A/Y456A, using D-glucose as cosubstrate, in 50 mM KH2PO4 buffer (pH 6.5), at 30°C | Trametes ochracea | |
510 | - |
ferrocenium hexafluorophosphate | wild type enzyme, using D-glucose as cosubstrate, in 50 mM KH2PO4 buffer (pH 6.5), at 30°C | Trametes ochracea | |
520 | - |
1,4-benzoquinone | mutant enzyme F454A/S455A/Y456A, using D-glucose as cosubstrate, in 50 mM KH2PO4 buffer (pH 6.5), at 30°C | Trametes ochracea | |
520 | - |
1,4-benzoquinone | mutant enzyme F454N, using D-galactose as cosubstrate, in 50 mM KH2PO4 buffer (pH 6.5), at 30°C | Trametes ochracea | |
940 | - |
1,4-benzoquinone | mutant enzyme H450Q, using D-glucose as cosubstrate, in 50 mM KH2PO4 buffer (pH 6.5), at 30°C | Trametes ochracea | |
1200 | - |
1,4-benzoquinone | wild type enzyme, using D-glucose as cosubstrate, in 50 mM KH2PO4 buffer (pH 6.5), at 30°C | Trametes ochracea | |
1210 | - |
ferrocenium hexafluorophosphate | mutant enzyme F454N, using D-glucose as cosubstrate, in 50 mM KH2PO4 buffer (pH 6.5), at 30°C | Trametes ochracea | |
1250 | - |
ferrocenium hexafluorophosphate | mutant enzyme H450Q, using D-glucose as cosubstrate, in 50 mM KH2PO4 buffer (pH 6.5), at 30°C | Trametes ochracea | |
1640 | - |
ferrocenium hexafluorophosphate | mutant enzyme Y456W, using D-glucose as cosubstrate, in 50 mM KH2PO4 buffer (pH 6.5), at 30°C | Trametes ochracea | |
2100 | - |
1,4-benzoquinone | mutant enzyme F454A/Y456A, using D-glucose as cosubstrate, in 50 mM KH2PO4 buffer (pH 6.5), at 30°C | Trametes ochracea | |
2600 | - |
1,4-benzoquinone | mutant enzyme F454N, using D-glucose as cosubstrate, in 50 mM KH2PO4 buffer (pH 6.5), at 30°C | Trametes ochracea | |
3000 | - |
1,4-benzoquinone | mutant enzyme Y456W, using D-glucose as cosubstrate, in 50 mM KH2PO4 buffer (pH 6.5), at 30°C | Trametes ochracea |