Inhibitors | Comment | Organism | Structure |
---|---|---|---|
N-bromosuccinimide | completely inhibitory at 1 mM | Phlebiopsis gigantea | |
p-chloromercuribenzoate | completely inhibits at 1 mM | Phlebiopsis gigantea |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
1.2 | - |
D-glucose | - |
Phlebiopsis gigantea | |
16.5 | - |
L-sorbose | - |
Phlebiopsis gigantea | |
22.2 | - |
D-xylose | - |
Phlebiopsis gigantea |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
70000 | - |
2 * 70000 + 2 * 77000, SDS-PAGE, electron microscopy | Phlebiopsis gigantea |
77000 | - |
2 * 70000 + 2 * 77000, SDS-PAGE, electron microscopy | Phlebiopsis gigantea |
273700 | - |
calculation on the basis of the Stoke's radius and sedimentation coefficient | Phlebiopsis gigantea |
280000 | - |
gel filtration | Phlebiopsis gigantea |
305000 | - |
native gradient gel electrophoresis | Phlebiopsis gigantea |
323600 | - |
electron microscopy | Phlebiopsis gigantea |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Phlebiopsis gigantea | - |
- |
- |
Purification (Comment) | Organism |
---|---|
using heat treatment, immunoaffinity chromatography and gel filtration on Superdex 200 | Phlebiopsis gigantea |
Source Tissue | Comment | Organism | Textmining |
---|
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
8.15 | - |
purified enzyme | Phlebiopsis gigantea |
Storage Stability | Organism |
---|---|
40°C, 10 mM sodium phosphate, pH 6-8, half life of enzyme activity: more than 300 days | Phlebiopsis gigantea |
50°C, 10 mM sodium phosphate, pH 7, half life of enzyme activity: more than 110 days | Phlebiopsis gigantea |
65°C, 10 mM sodium phosphate, pH 7, half life of enzyme activity: 1 h | Phlebiopsis gigantea |
inactivated by freezing and thawing | Phlebiopsis gigantea |
room temperature and 30°C, pH 5-10, half life of enzyme activity: more than 300 days | Phlebiopsis gigantea |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
D-galactose + O2 | 8.5% relative activity to D-glucose | Phlebiopsis gigantea | 2-dehydro-D-galactose + H2O2 | - |
? | |
D-glucose + O2 | 100% relative activity | Phlebiopsis gigantea | D-glucosone + H2O2 | - |
? | |
D-glucose + O2 | the activation energy for D-glucose activation is 24.7 kJ/mol | Phlebiopsis gigantea | D-glucosone + H2O2 | - |
? | |
D-glucose + O2 | D-glucose is the preferred substrate | Phlebiopsis gigantea | D-glucosone + H2O2 | - |
? | |
D-xylose + O2 | 43.7% relative activity to D-glucose | Phlebiopsis gigantea | D-xylosone + H2O2 | - |
? | |
L-sorbose + O2 | 96.3% relative activity to D-glucose | Phlebiopsis gigantea | 5-dehydro-D-fructose + H2O2 | - |
? | |
methyl alpha-D-glucoside + O2 | 3% relative activity to D-glucose | Phlebiopsis gigantea | 2-dehydro-alpha-D-methylglucoside + H2O2 | - |
? | |
methyl beta-D-glucoside + O2 | 26.3% relative activity to D-glucose | Phlebiopsis gigantea | 2-dehydro-beta-D-methylglucoside + H2O2 | - |
? |
Subunits | Comment | Organism |
---|---|---|
heterotetramer | 2 * 70000 + 2 * 77000, SDS-PAGE, electron microscopy | Phlebiopsis gigantea |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
65 | - |
- |
Phlebiopsis gigantea |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
5 | 8 | in 100 mM sodium phosphate | Phlebiopsis gigantea |
5 | 7.5 | in 100 mM potassium phosphate | Phlebiopsis gigantea |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
FAD | covalently bound | Phlebiopsis gigantea | |
FAD | 3.12 mol FAD per mol of enzyme | Phlebiopsis gigantea |