Any feedback?
Literature summary for 1.1.2.4 extracted from
- D-Lactate dehydrogenase of Desulfovibrio vulgaris (1981), J. Biochem., 89, 1423-1431.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| EDTA | slightly stimulating | Desulfovibrio vulgaris |  |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| cyanide | - |
Desulfovibrio vulgaris | |
| EDTA | no inhibitor | Desulfovibrio vulgaris | |
| o-phenanthroline | very strong | Desulfovibrio vulgaris | |
| oxalate | - |
Desulfovibrio vulgaris | |
| p-chloromercuribenzoate | weak | Desulfovibrio vulgaris | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.8 | - |
D-lactate | - |
Desulfovibrio vulgaris | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| additional information | equally distributed between soluble and membrane fraction | Desulfovibrio vulgaris | - |
- |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| (R)-lactate + 2 ferricytochrome c | Desulfovibrio vulgaris | required for growth on lactate as sole carbon source | pyruvate + ferrocytochrome c | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Desulfovibrio vulgaris | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Desulfovibrio vulgaris |
Storage Stability
| Storage Stability | Organism |
|---|---|
| 4°C, 0.1 M Tris-buffer, pH 7.3, 20% loss of activity | Desulfovibrio vulgaris |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| (R)-lactate + 2 ferricytochrome c | required for growth on lactate as sole carbon source | Desulfovibrio vulgaris | pyruvate + ferrocytochrome c | - |
? | |
| (R)-lactate + ferricytochrome c | traces of activity with L-isomer | Desulfovibrio vulgaris | pyruvate + ferrocytochrome c | - |
? | |
| D-2-hydroxybutyrate + cytochrome c | - |
Desulfovibrio vulgaris | 2-oxobutanoate + ferrocytochrome c | - |
? | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.5 | 9 | - |
Desulfovibrio vulgaris |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 5 | 9 | no activity at pH 5, almost maximum activity at pH 9 | Desulfovibrio vulgaris |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| 2,6-dichlorophenolindophenol | can substitute for cytochrome c | Desulfovibrio vulgaris | |
| cytochrome c | only cytochrome c-553 from same organism, eucaryotic cytochromes are not reduced | Desulfovibrio vulgaris | |
| additional information | no activity with NAD+ or NADP+ | Desulfovibrio vulgaris | |
| phenazine methosulfate | can substitute for cytochrome c | Desulfovibrio vulgaris | |
Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.
Release 2023.1 (January 2023)
Legal
Curated at
Member of
