Cloned (Comment) | Organism |
---|---|
in Escherichia coli AR120 | Saccharomyces cerevisiae |
Protein Variants | Comment | Organism |
---|---|---|
additional information | three different deletion mutants with deletion of 3, 6 and 9 amino acids from hinge region | Saccharomyces cerevisiae |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Fe2+ | bound to enzyme | Saccharomyces cerevisiae |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
57000 | - |
4 * 57000 | Saccharomyces cerevisiae |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
(S)-lactate + 2 ferricytochrome c | Saccharomyces cerevisiae | can feed electrons to respiratory chain at the level of cytochrome c | pyruvate + 2 ferrocytochrome c + 2 H+ | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Saccharomyces cerevisiae | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Saccharomyces cerevisiae |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
(S)-lactate + 2 ferricytochrome c | - |
Saccharomyces cerevisiae | pyruvate + 2 ferrocytochrome c + 2 H+ | - |
? | |
(S)-lactate + 2 ferricytochrome c | can feed electrons to respiratory chain at the level of cytochrome c | Saccharomyces cerevisiae | pyruvate + 2 ferrocytochrome c + 2 H+ | - |
? | |
additional information | ferricyanide used as electron acceptor | Saccharomyces cerevisiae | ? | - |
? | |
additional information | electron acceptors other than ferricytochrome c used | Saccharomyces cerevisiae | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
tetramer | 4 * 57000 | Saccharomyces cerevisiae |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | systematic determination of turnover numbers for wild type enzyme and deletion mutants with different electron acceptors, significantly reduced activity for deletion mutants with cytochrome c as electron acceptor | Saccharomyces cerevisiae |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
cytochrome c | - |
Saccharomyces cerevisiae | |
additional information | ferricyanide used as electron acceptor | Saccharomyces cerevisiae | |
additional information | electron acceptors other than ferricytochrome c used | Saccharomyces cerevisiae |