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Literature summary for 1.1.2.3 extracted from

  • Balme, A.; Lederer, F.
    On the rate of proton exchange with solvent of the catalytic histidine in flavocytochrome b2 (yeast L-lactate dehydrogenase) (1994), Protein Sci., 3, 109-117.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 1.1.2.3

Cloned(Commentary)

Cloned (Comment) Organism
-
 Saccharomyces cerevisiae

Protein Variants

Protein Variants Comment Organism
Y254del deletion mutant Saccharomyces cerevisiae
Y254F
-
 Saccharomyces cerevisiae

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.54
-
 L-lactate
-
 Saccharomyces cerevisiae
3.9
-
 L-lactate Y254F mutant Saccharomyces cerevisiae
6
-
 ferricyanide
-
 Saccharomyces cerevisiae
8.9
-
 L-lactate wild type enzyme Saccharomyces cerevisiae

Metals/Ions

Metals/Ions Comment Organism Structure
Fe2+ bound to enzyme Saccharomyces cerevisiae

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
(S)-lactate + 2 ferricytochrome c Saccharomyces cerevisiae can feed electrons to respiratory chain at the level of cytochrome c pyruvate + 2 ferrocytochrome c + 2 H+
-
 r

Organism

Organism UniProt Comment Textmining
Saccharomyces cerevisiae
-
-
-

Storage Stability

Storage Stability Organism
-80°C, 0.1 M phosphate buffer, 1 mM EDTA, 1 mM PMSF Saccharomyces cerevisiae

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(S)-lactate + 2 ferricytochrome c
-
 Saccharomyces cerevisiae pyruvate + 2 ferrocytochrome c + 2 H+
-
 r
(S)-lactate + 2 ferricytochrome c can feed electrons to respiratory chain at the level of cytochrome c Saccharomyces cerevisiae pyruvate + 2 ferrocytochrome c + 2 H+
-
 r
fluoropyruvate + ferrocytochrome c + H+
-
 Saccharomyces cerevisiae ?
-
 ?
L-lactate + ferricyanide
-
 Saccharomyces cerevisiae pyruvate + ferrocyanide + H+
-
 ?
additional information electron acceptors other than ferricytochrome c used Saccharomyces cerevisiae ?
-
 ?

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.2
-
 Fluoropyruvate Y254F mutant enzyme Saccharomyces cerevisiae
2.8
-
 Fluoropyruvate wild type enzyme Saccharomyces cerevisiae
473
-
 L-lactate wild type enzyme Saccharomyces cerevisiae

Cofactor

Cofactor Comment Organism Structure
additional information electron acceptors other than ferricytochrome c used Saccharomyces cerevisiae