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Literature summary for 1.1.2.3 extracted from

  • Silvestrini, M.C.; Tegoni, M.; Celerier, J.; Desbois, A.; Gervais, M.
    Expression in Escherichia coli of the flavin and the haem domains of Hansenula anomala flavocytochrome b2 (flavodehydrogenase and b2 core) and characterization of the recombinant proteins (1993), Biochem. J., 295, 501-508.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

Cloned (Comment) Organism
flavin and heme domain expressed in Escherichia coli Wickerhamomyces anomalus

Inhibitors

Inhibitors Comment Organism Structure
L-lactate 40% inhibition at 60 mM Wickerhamomyces anomalus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3
-
L-lactate with ferricyanide as electron acceptor Wickerhamomyces anomalus

Metals/Ions

Metals/Ions Comment Organism Structure
Fe2+ bound to enzyme Wickerhamomyces anomalus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
(S)-lactate + 2 ferricytochrome c Wickerhamomyces anomalus
-
pyruvate + 2 ferrocytochrome c + 2 H+
-
?

Organism

Organism UniProt Comment Textmining
Wickerhamomyces anomalus
-
flavin and heme domain expressed in Escherichia coli
-

Purification (Commentary)

Purification (Comment) Organism
flavin and heme domain from cloned Escherichia coli Wickerhamomyces anomalus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(S)-lactate + 2 ferricytochrome c
-
Wickerhamomyces anomalus pyruvate + 2 ferrocytochrome c + 2 H+
-
?
additional information ferricyanide used as electron acceptor Wickerhamomyces anomalus ?
-
?
additional information electron acceptors other than ferricytochrome c used Wickerhamomyces anomalus ?
-
?

Cofactor

Cofactor Comment Organism Structure
additional information ferricyanide used as electron acceptor Wickerhamomyces anomalus
additional information electron acceptors other than ferricytochrome c used Wickerhamomyces anomalus