Cloned (Comment) | Organism |
---|---|
- |
Saccharomyces cerevisiae |
Crystallization (Comment) | Organism |
---|---|
- |
Saccharomyces cerevisiae |
Protein Variants | Comment | Organism |
---|---|---|
R376K | mutant enzyme shows no activity | Saccharomyces cerevisiae |
Y254F | significantly reduced activity of mutant enzyme | Saccharomyces cerevisiae |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.084 | - |
Phenyllactate | Y254F mutant | Saccharomyces cerevisiae | |
0.35 | - |
L-lactate | Y254F mutant | Saccharomyces cerevisiae | |
0.4 | - |
Phenyllactate | wild type enzyme | Saccharomyces cerevisiae | |
0.49 | - |
L-lactate | wild type enzyme | Saccharomyces cerevisiae |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Fe2+ | bound to enzyme | Saccharomyces cerevisiae |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
(S)-lactate + 2 ferricytochrome c | Saccharomyces cerevisiae | can feed electrons to respiratory chain at the level of cytochrome c | pyruvate + 2 ferrocytochrome c + 2 H+ | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Saccharomyces cerevisiae | - |
- |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
(S)-lactate + 2 ferricytochrome c | - |
Saccharomyces cerevisiae | pyruvate + 2 ferrocytochrome c + 2 H+ | - |
? | |
(S)-lactate + 2 ferricytochrome c | can feed electrons to respiratory chain at the level of cytochrome c | Saccharomyces cerevisiae | pyruvate + 2 ferrocytochrome c + 2 H+ | - |
? | |
additional information | electron acceptors other than ferricytochrome c used | Saccharomyces cerevisiae | ? | - |
? | |
phenyllactate + ferricytochrome c | - |
Saccharomyces cerevisiae | phenylpyruvate + ferrocytochrome c | - |
? |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.29 | - |
Phenyllactate | Y254F mutant enzyme | Saccharomyces cerevisiae | |
6.1 | - |
L-lactate | Y254F mutant enzyme | Saccharomyces cerevisiae | |
16.5 | - |
Phenyllactate | wild type enzyme | Saccharomyces cerevisiae | |
270 | - |
L-lactate | wild type enzyme | Saccharomyces cerevisiae |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
FMN | - |
Saccharomyces cerevisiae | |
heme | - |
Saccharomyces cerevisiae | |
additional information | electron acceptors other than ferricytochrome c used | Saccharomyces cerevisiae |