Inhibitors | Comment | Organism | Structure |
---|---|---|---|
oxalate | complete inhibition, competitive | Rhizopus arrhizus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
3.85 | - |
L-lactate | 2,6-dichlorophenolindopenol as electron acceptor | Rhizopus arrhizus |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Fe2+ | bound to enzyme | Rhizopus arrhizus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
(S)-lactate + 2 ferricytochrome c | Rhizopus arrhizus | replaces activity of EC 1.1.1.27 in limited substrate conditions | pyruvate + 2 ferrocytochrome c + 2 H+ | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Rhizopus arrhizus | - |
- |
- |
Purification (Comment) | Organism |
---|---|
partial | Rhizopus arrhizus |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.055 | - |
(S)-lactate with cytochrome c as electron acceptor | Rhizopus arrhizus |
0.415 | - |
partially purified enzyme with 2,6-dichloroindophenol as electron acceptor | Rhizopus arrhizus |
0.72 | - |
6-dichloroindophenol as electron acceptor | Rhizopus arrhizus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
(S)-lactate + 2 ferricytochrome c | D-isomer not oxidized | Rhizopus arrhizus | pyruvate + 2 ferrocytochrome c + 2 H+ | - |
? | |
(S)-lactate + 2 ferricytochrome c | replaces activity of EC 1.1.1.27 in limited substrate conditions | Rhizopus arrhizus | pyruvate + 2 ferrocytochrome c + 2 H+ | - |
? | |
additional information | 2,6-dichlorophenolindophenol, ferricyanide and cytochrome c but not oxygen can serve as electron acceptor, rate of reduction with cytochrome c much slower than with the other possible acceptors, glycolate and L-malate do not serve as substrates | Rhizopus arrhizus | ? | - |
? | |
additional information | electron acceptors other than ferricytochrome c used | Rhizopus arrhizus | ? | - |
? |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.2 | 8.4 | - |
Rhizopus arrhizus |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
5.2 | - |
almost no activity | Rhizopus arrhizus |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
cytochrome c | - |
Rhizopus arrhizus | |
additional information | electron acceptors other than ferricytochrome c used | Rhizopus arrhizus | |
additional information | NAD+ and NADP+ cannot substitute, 2,6-dichlorophenolindophenol, ferricyanide and cytochrome c but not oxygen can serve as electron acceptor, rate of reduction with cytochrome c much slower than with the other possible acceptors | Rhizopus arrhizus |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
0.4 | - |
oxalate | 10 mM | Rhizopus arrhizus | |
0.445 | - |
oxalate | 1 mM | Rhizopus arrhizus |