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Literature summary for 1.1.1.B38 extracted from

  • Kim, D.Y.; Stauffacher, C.V.; Rodwell, V.W.
    Dual coenzyme specificity of Archaeoglobus fulgidus HMG-CoA reductase (2000), Protein Sci., 9, 1226-1234.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Archaeoglobus fulgidus

Inhibitors

Inhibitors Comment Organism Structure
Mevinolin competitive Archaeoglobus fulgidus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.16
-
NADH pH 9.0, 50°C Archaeoglobus fulgidus
0.5
-
NADPH pH 6.0, 50°C Archaeoglobus fulgidus
0.5
-
NAD+ pH 10.0, 50°C Archaeoglobus fulgidus
0.62
-
mevalonate 50°C, pH not specified in the publication Archaeoglobus fulgidus
1.7
-
NADP+ pH 7.0, 50°C Archaeoglobus fulgidus

Organism

Organism UniProt Comment Textmining
Archaeoglobus fulgidus O28538
-
-

Purification (Commentary)

Purification (Comment) Organism
-
Archaeoglobus fulgidus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
mevalonate + CoA + 2 NAD+ optimal activity using NADP(H) occurs at pH from 1 to 3 units more acidic than that observed using NAD(H) Archaeoglobus fulgidus 3-hydroxy-3-methylglutaryl-CoA + 2 NADH + 2 H+
-
r
mevalonate + CoA + 2 NADP+ slight preference for NAD(H) versus NADP(H). Optimal activity using NADP(H) occurs at a pH from 1 to 3 units more acidic than that observed using NAD(H) Archaeoglobus fulgidus 3-hydroxy-3-methylglutaryl-CoA + 2 NADPH + 2 H+
-
r

Synonyms

Synonyms Comment Organism
AF1736
-
Archaeoglobus fulgidus
HMG-CoA reductase
-
Archaeoglobus fulgidus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
85
-
-
Archaeoglobus fulgidus

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
65 95 65°C: about 45% of maximal activity, 95°C: about 65% of maximal activity Archaeoglobus fulgidus

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.08
-
NADH pH 9.0, 50°C Archaeoglobus fulgidus
0.1
-
NAD+ pH 10.0, 50°C Archaeoglobus fulgidus
2
-
NADPH pH 6.0, 50°C Archaeoglobus fulgidus
6.8
-
NADP+ pH 7.0, 50°C Archaeoglobus fulgidus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6
-
reductive deacylation of 3-hydroxy-3-methylglutaryl-CoA to mevalonate with NADPH Archaeoglobus fulgidus
6.8
-
oxidative acylation of mevalonate to 3-hydroxy-3-methylglutaryl-CoA with NADP+ Archaeoglobus fulgidus
9
-
reductive deacylation of 3-hydroxy-3-methylglutaryl-CoA to mevalonate with NADH Archaeoglobus fulgidus
9.5 10 oxidative acylation of mevalonate to 3-hydroxy-3-methylglutaryl-CoA with NAD+ Archaeoglobus fulgidus

Cofactor

Cofactor Comment Organism Structure
NAD+ slight preference for NAD(H) versus NADP(H). Optimal activity using NADP(H) occurrs at a pH from 1 to 3 units more acidic than that observed using NAD(H). Each coenzyme acts as a competitive inhibitor of the other Archaeoglobus fulgidus
NADH slight preference for NAD(H) versus NADP(H). Optimal activity using NADP(H) occurrs at a pH from 1 to 3 units more acidic than that observed using NAD(H). Each coenzyme acts as a competitive inhibitor of the other Archaeoglobus fulgidus
NADP+ slight preference for NAD(H) versus NADP(H). Optimal activity using NADP(H) occurrs at a pH from 1 to 3 units more acidic than that observed using NAD(H). Each coenzyme acts as a competitive inhibitor of the other Archaeoglobus fulgidus
NADPH slight preference for NAD(H) versus NADP(H). Optimal activity using NADP(H) occurrs at a pH from 1 to 3 units more acidic than that observed using NAD(H). Each coenzyme acts as a competitive inhibitor of the other Archaeoglobus fulgidus

IC50 Value

IC50 Value IC50 Value Maximum Comment Organism Inhibitor Structure
0.18
-
50°C, pH not specified in the publication Archaeoglobus fulgidus Mevinolin

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
0.25
-
NADP+ pH 7.0, 50°C Archaeoglobus fulgidus
1
-
NADPH pH 6.0, 50°C Archaeoglobus fulgidus
2
-
NADH pH 9.0, 50°C Archaeoglobus fulgidus
5
-
NAD+ pH 10.0, 50°C Archaeoglobus fulgidus