Cloned (Comment) | Organism |
---|---|
gene bdh1, phylogenetic analysis, expression in Escherichia coi strain YYC202(DE3) ldhA-/- ilvC? expressing ilvBN from Escherichia coli and aldB from Lactobacillus lactis, encoding acetolactate synthase and acetolactate decarboxylase activities, respectively, disruption of the lactate biosynthesis pathway in the strain increases pyruvate precursor availability to this strain, increased availability of NADH for acetoin reduction to meso-2,3-butanediol is the most important consequence of ldhA deletion | Saccharomyces cerevisiae |
phylogenetic analysis, enzyme expression in Escherichia coli strains MG1655(DE3) and YYC202(DE3) | Escherichia coli |
Protein Variants | Comment | Organism |
---|---|---|
additional information | expression of gene bdh1 from Saccharomyces cervisiae in Escherichia coi strain YYC202(DE3) ldhA-/- ilvC-/- expressing ilvBN from Escherichia coli and aldB from Lactobacillus lactis, encoding acetolactate synthase and acetolactate decarboxylase activities, respectively. Disruption of the lactate biosynthesis pathway in the strain increases pyruvate precursor availability to this strain, increased availability of NADH for acetoin reduction to meso-2,3-butanediol is the most important consequence of ldhA deletion. Optimization of 2,3-butanediol production in Escherichia coli, overview | Escherichia coli |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
(3R)-acetoin + NADH + H+ | Saccharomyces cerevisiae | - |
meso-2,3-butanediol + NAD+ | - |
r | |
(3R)-acetoin + NADH + H+ | Escherichia coli | low activity in vivo | meso-2,3-butanediol + NAD+ | - |
r |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | - |
- |
- |
Saccharomyces cerevisiae | - |
gene bdh1 | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
(3R)-acetoin + NADH + H+ | - |
Escherichia coli | meso-2,3-butanediol + NAD+ | - |
r | |
(3R)-acetoin + NADH + H+ | - |
Saccharomyces cerevisiae | meso-2,3-butanediol + NAD+ | - |
r | |
(3R)-acetoin + NADH + H+ | low activity in vivo | Escherichia coli | meso-2,3-butanediol + NAD+ | - |
r |
Synonyms | Comment | Organism |
---|---|---|
2,3-BD dehydrogenase | - |
Escherichia coli |
2,3-BD dehydrogenase | - |
Saccharomyces cerevisiae |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NADH | - |
Escherichia coli | |
NADH | - |
Saccharomyces cerevisiae |
General Information | Comment | Organism |
---|---|---|
evolution | phylogenetic analysis | Escherichia coli |
evolution | phylogenetic analysis | Saccharomyces cerevisiae |