Application | Comment | Organism |
---|---|---|
synthesis | coversion of 1-(3-hydroxyphenyl)-2-(methylamino) ethanone to (S)-phenylephrine with with more than 99% enantiomeric excess, 78% yield and a productivity of 3.9 mmol(S)-phenylepinephrine/l h in 12 h at 30°C and pH 7. The (S)-phenylepinephrine, recovered from reaction mixture by precipitation at pH 11.3, can be converted to (R)-phenylepinephrine by Walden inversion reaction | Rhodococcus erythropolis |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
26580 | - |
x * 26580, calcuulated, x * 30000, SDS-PAGE | Rhodococcus erythropolis |
30000 | - |
x * 26580, calcuulated, x * 30000, SDS-PAGE | Rhodococcus erythropolis |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Rhodococcus erythropolis | D6MMN3 | - |
- |
Rhodococcus erythropolis BCRC 10909 | D6MMN3 | - |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
(2S)-2-(methylamino)-1-phenylpropan-1-one + NADPH + H+ | - |
Rhodococcus erythropolis | (1S,2S)-2-(methylamino)-1-phenylpropan-1-ol + NADP+ | conversion with with more than 99% enantiomeric excess, 78% yield and a productivity of 3.9 mmol(S)-phenylepinephrine/l h in 12 h at 30°C and pH 7 | ? | |
(2S)-2-(methylamino)-1-phenylpropan-1-one + NADPH + H+ | - |
Rhodococcus erythropolis BCRC 10909 | (1S,2S)-2-(methylamino)-1-phenylpropan-1-ol + NADP+ | conversion with with more than 99% enantiomeric excess, 78% yield and a productivity of 3.9 mmol(S)-phenylepinephrine/l h in 12 h at 30°C and pH 7 | ? |
Subunits | Comment | Organism |
---|---|---|
? | x * 26580, calcuulated, x * 30000, SDS-PAGE | Rhodococcus erythropolis |
Synonyms | Comment | Organism |
---|---|---|
amino alcohol dehydrogenase | - |
Rhodococcus erythropolis |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NADPH | specific for NADPH | Rhodococcus erythropolis |