Literature summary for 1.1.1.B18 extracted from

Kataoka, M.; Ishige, T.; Urano, N.; Nakamura, Y.; Sakuradani, E.; Fukui, S.; Kita, S.; Sakamoto, K.; Shimizu, S.
Cloning and expression of the L-1-amino-2-propanol dehydrogenase gene from Rhodococcus erythropolis, and its application to double chiral compound production (2008), Appl. Microbiol. Biotechnol., 80, 597-604.

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Cloned(Commentary)

Cloned (Comment)	Organism
gene aadh, DNA and amino acid sequence determination and analysis, expression in Escherichia coli JM109 leading to a 10.4fold increase in specific activity as to catalysis of the reduction of (2S)-2-(methylamino)-1-phenylpropan-1-one, as compared with that of Rhodococcus erythropolis strain MAK154 induced by 1-amino-2-propanol. Coexpression of aadh with glucose dehydrogenase gene gdh as cofactor regeneration enzyme, and installation of a system for sufficient production of d-psi-pseudoephedrine from racemic (S)-1-phenyl-2-methylaminopropan-1-one	Rhodococcus erythropolis

Cloned (Comment)

Organism

gene aadh, DNA and amino acid sequence determination and analysis, expression in Escherichia coli JM109 leading to a 10.4fold increase in specific activity as to catalysis of the reduction of (2S)-2-(methylamino)-1-phenylpropan-1-one, as compared with that of Rhodococcus erythropolis strain MAK154 induced by 1-amino-2-propanol. Coexpression of aadh with glucose dehydrogenase gene gdh as cofactor regeneration enzyme, and installation of a system for sufficient production of d-psi-pseudoephedrine from racemic (S)-1-phenyl-2-methylaminopropan-1-one

Rhodococcus erythropolis

Inhibitors

Inhibitors	Comment	Organism	Structure
(1S,2S)-2-(methylamino)-1-phenylpropan-1-ol	product inhibition above 20 mg/ml	Rhodococcus erythropolis

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
26580	-	x * 26580, calculated	Rhodococcus erythropolis

Organism

Organism	UniProt	Comment	Textmining
Rhodococcus erythropolis	A1IG83	-	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
(2S)-2-(methylamino)-1-phenylpropan-1-one + NADPH + H+	diastereoselective reduction	Rhodococcus erythropolis	(1S,2S)-2-(methylamino)-1-phenylpropan-1-ol + NADP+	i.e. d-psi-pseudoephedrine, molar reaction yields of 89.1% and more than 99% enantiomeric and diastereomeric excess	?

Subunits

Subunits	Comment	Organism
?	x * 26580, calculated	Rhodococcus erythropolis

Synonyms

Synonyms	Comment	Organism
AADH	-	Rhodococcus erythropolis
L-1-amino-2-propanol dehydrogenase	-	Rhodococcus erythropolis

Temperature Optimum [°C]

Temperature Optimum [°C]	Temperature Optimum Maximum [°C]	Comment	Organism
37	-	assay at	Rhodococcus erythropolis

pH Optimum

pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
8	-	assay at	Rhodococcus erythropolis