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Literature summary for 1.1.1.95 extracted from

  • Benstein, R.M.; Ludewig, K.; Wulfert, S.; Wittek, S.; Gigolashvili, T.; Frerigmann, H.; Gierth, M.; Fluegge, U.I.; Krueger, S.
    Arabidopsis phosphoglycerate dehydrogenase1 of the phosphoserine pathway is essential for development and required for ammonium assimilation and tryptophan biosynthesis (2013), Plant Cell, 25, 5011-5029.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 1.1.1.95

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL-21 cells Arabidopsis thaliana

Inhibitors

Inhibitors Comment Organism Structure
L-serine
-
 Arabidopsis thaliana

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.189
-
 NAD+ isoform PGDH2, at pH 8.1 and 37°C Arabidopsis thaliana
0.239
-
 NAD+ isoform PGDH3, at pH 8.1 and 37°C Arabidopsis thaliana
0.271
-
 NAD+ isoform PGDH2, at pH 7.2 and 37°C Arabidopsis thaliana
0.377
-
 NAD+ isoform PGDH1, at pH 7.2 and 37°C Arabidopsis thaliana
0.39
-
 NAD+ isoform PGDH1, at pH 8.1 and 37°C Arabidopsis thaliana
0.551
-
 NAD+ isoform PGDH3, at pH 7.2 and 37°C Arabidopsis thaliana
0.899
-
 3-phospho-D-glycerate isoform PGDH2, at pH 8.1 and 37°C Arabidopsis thaliana
1.006
-
 3-phospho-D-glycerate isoform PGDH3, at pH 8.1 and 37°C Arabidopsis thaliana
1.308
-
 3-phospho-D-glycerate isoform PGDH1, at pH 8.1 and 37°C Arabidopsis thaliana
1.926
-
 3-phospho-D-glycerate isoform PGDH2, at pH 7.2 and 37°C Arabidopsis thaliana
2.11
-
 3-phospho-D-glycerate isoform PGDH1, at pH 7.2 and 37°C Arabidopsis thaliana
2.559
-
 3-phospho-D-glycerate isoform PGDH3, at pH 7.2 and 37°C Arabidopsis thaliana

Localization

Localization Comment Organism GeneOntology No. Textmining
chloroplast
-
 Arabidopsis thaliana 9507
-

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3-phospho-D-glycerate + NAD+ Arabidopsis thaliana
-
 3-phosphonooxypyruvate + NADH + H+
-
 ?

Organism

Organism UniProt Comment Textmining
Arabidopsis thaliana O04130 ecotype Col-0
-
Arabidopsis thaliana O49485 ecotype Col-0
-
Arabidopsis thaliana Q9LT69 ecotype Col-0
-

Purification (Commentary)

Purification (Comment) Organism
Ni-NTA column chromatography Arabidopsis thaliana

Source Tissue

Source Tissue Comment Organism Textmining
embryo
-
 Arabidopsis thaliana
-
flower
-
 Arabidopsis thaliana
-
leaf
-
 Arabidopsis thaliana
-
root
-
 Arabidopsis thaliana
-
seedling
-
 Arabidopsis thaliana
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3-phospho-D-glycerate + NAD+
-
 Arabidopsis thaliana 3-phosphonooxypyruvate + NADH + H+
-
 ?

Synonyms

Synonyms Comment Organism
PGDH1 isoform Arabidopsis thaliana
PGDH2 isoform Arabidopsis thaliana
PGDH3 isoform Arabidopsis thaliana

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.741
-
 NAD+ isoform PGDH1, at pH 8.1 and 37°C Arabidopsis thaliana
1.741
-
 3-phospho-D-glycerate isoform PGDH1, at pH 8.1 and 37°C Arabidopsis thaliana
97
-
 NAD+ isoform PGDH3, at pH 7.2 and 37°C Arabidopsis thaliana
97
-
 3-phospho-D-glycerate isoform PGDH3, at pH 7.2 and 37°C Arabidopsis thaliana
115.1
-
 NAD+ isoform PGDH1, at pH 7.2 and 37°C Arabidopsis thaliana
115.1
-
 3-phospho-D-glycerate isoform PGDH1, at pH 7.2 and 37°C Arabidopsis thaliana
119.7
-
 NAD+ isoform PGDH2, at pH 7.2 and 37°C Arabidopsis thaliana
119.7
-
 3-phospho-D-glycerate isoform PGDH2, at pH 7.2 and 37°C Arabidopsis thaliana
142.4
-
 NAD+ isoform PGDH3, at pH 8.1 and 37°C Arabidopsis thaliana
142.4
-
 3-phospho-D-glycerate isoform PGDH3, at pH 8.1 and 37°C Arabidopsis thaliana
147.4
-
 NAD+ isoform PGDH2, at pH 8.1 and 37°C Arabidopsis thaliana
147.4
-
 3-phospho-D-glycerate isoform PGDH2, at pH 8.1 and 37°C Arabidopsis thaliana

Cofactor

Cofactor Comment Organism Structure
NAD+
-
 Arabidopsis thaliana

General Information

General Information Comment Organism
malfunction PGDH1-silenced lines are inhibited in growth Arabidopsis thaliana

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
37.91
-
 3-phospho-D-glycerate isoform PGDH3, at pH 7.2 and 37°C Arabidopsis thaliana
54.57
-
 3-phospho-D-glycerate isoform PGDH2, at pH 8.1 and 37°C Arabidopsis thaliana
62.16
-
 3-phospho-D-glycerate isoform PGDH1, at pH 7.2 and 37°C Arabidopsis thaliana
133.1
-
 3-phospho-D-glycerate isoform PGDH1, at pH 8.1 and 37°C Arabidopsis thaliana
141.6
-
 3-phospho-D-glycerate isoform PGDH2, at pH 7.2 and 37°C Arabidopsis thaliana
154
-
 3-phospho-D-glycerate isoform PGDH3, at pH 8.1 and 37°C Arabidopsis thaliana
176.1
-
 NAD+ isoform PGDH3, at pH 7.2 and 37°C Arabidopsis thaliana
305.7
-
 NAD+ isoform PGDH2, at pH 8.1 and 37°C Arabidopsis thaliana
442.6
-
 NAD+ isoform PGDH1, at pH 7.2 and 37°C Arabidopsis thaliana
446.3
-
 NAD+ isoform PGDH1, at pH 8.1 and 37°C Arabidopsis thaliana
595
-
 NAD+ isoform PGDH2, at pH 7.2 and 37°C Arabidopsis thaliana
780.1
-
 NAD+ isoform PGDH3, at pH 8.1 and 37°C Arabidopsis thaliana