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Literature summary for 1.1.1.91 extracted from

  • Magomedova, Z.; Grecu, A.; Sensen, C.W.; Schwab, H.; Heidinger, P.
    Characterization of two novel alcohol short-chain dehydrogenases/reductases from Ralstonia eutropha H16 capable of stereoselective conversion of bulky substrates (2016), J. Biotechnol., 221, 78-90.
    View publication on PubMed
Search for more literature for 1.1.1.91

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli Top10 cells Cupriavidus necator

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
7.1
-
 (S)-2-octanol isoform SDR B3, at pH 10.0 and 40°C Cupriavidus necator
7.7
-
 (S)-2-octanol isoform SDR A5, at pH 10.0 and 40°C Cupriavidus necator

Organism

Organism UniProt Comment Textmining
Cupriavidus necator
-
-
-
Cupriavidus necator H16 / ATCC 23440 / NCIB 10442 / S-10-1
-
-
-

Purification (Commentary)

Purification (Comment) Organism
Ni-Sepharose column chromatography Cupriavidus necator

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(R)-2-octanol + NADPH + H+
-
 Cupriavidus necator ?
-
 ?
(R)-2-octanol + NADPH + H+
-
 Cupriavidus necator H16 / ATCC 23440 / NCIB 10442 / S-10-1 ?
-
 ?
(S)-2-octanol + NADPH + H+
-
 Cupriavidus necator ?
-
 ?
(S)-2-octanol + NADPH + H+
-
 Cupriavidus necator H16 / ATCC 23440 / NCIB 10442 / S-10-1 ?
-
 ?
2-methyl-propanol + NADPH + H+
-
 Cupriavidus necator ?
-
 ?
2-methyl-propanol + NADPH + H+
-
 Cupriavidus necator H16 / ATCC 23440 / NCIB 10442 / S-10-1 ?
-
 ?
3-methyl-butanol + NADPH + H+
-
 Cupriavidus necator ?
-
 ?
benzil + NADPH + H+
-
 Cupriavidus necator benzoin + NADP+
-
 ?
butanol + NADPH + H+
-
 Cupriavidus necator ?
-
 ?
additional information no activity is detected for isoform SDR A5 with isobutyraldehyde, isovaleraldehyde, 2-heptanone, 2-phenylacetaldehyde, 1-phenyl-1-butanone, 1-phenyl-1-pentanone, 1-phenyl-1-hexanone, 1-phenyl-2-propanone, 2-hydroxyacetophenone, 2'-hydroxyacetophenone, 1-phenylpropane-1,2-dione, benzophenone, (R)-(-)-benzoin, propanol, butanol, rac-2-propanol, 2-methylpropanol, 3-methylbutanol, rac-3-methyl-2-butanol, (S)-4-methyl-2-pentanol, (R)-4-methyl-2-pentanol, (R)-2-hexanol, (R)-2-octanol, rac-1-amino-2-propanol, rac-1-bromo-2-propanol, butan-2,3-diol, rac-decan-1,2-diol, 3-hydroxybutanoic acid, 2-hexyn-1-ol, (R)-1-phenylethanol, 2-phenylethanol, rac-benzoin, meso-hydrobenzoin, (S,S)-hydrobenzoin, and (R,R)-hydrobenzoin. No activity is detected for isoform SDR B3 with isobutyraldehyde, 2-hexanone, 2-heptanone, 2-octanone, ethyl acetoacetate, acetophenone, 1-phenyl-1-butanone, 1-phenyl-1-pentanone, 1-phenyl-1-hexanone, 1-phenyl-2-propanone, 2-hydroxyacetophenone, 2'-hydroxyacetophenone, 4-(4-hydroxyphenyl)-2-butanone, 1-phenylpropane-1,2-dione, benzophenone, rac-benzoin, (R)-benzoin, propanol, butanol, rac-2-propanol, 2-methylpropanol, 3-methylbutanol, (R)-4-methyl-2-pentanol, (R)-2-hexanol, rac-1-amino-2-propanol, rac-1-bromo-2-propanol, butan-2,3-diol, 3-hydroxybutanoic acid, 2-hexyn-1-ol, (R)-1-phenylethanol, 2-phenylethanol, rac-benzoin, meso-hydrobenzoin, (S,S)-hydrobenzoin, and (R,R)-hydrobenzoin Cupriavidus necator ?
-
 ?
additional information the enzyme accepts both NAD+ and NADP+. However, purified enzyme shows strong preference for NADP+ Cupriavidus necator ?
-
 ?
additional information no activity is detected for isoform SDR A5 with isobutyraldehyde, isovaleraldehyde, 2-heptanone, 2-phenylacetaldehyde, 1-phenyl-1-butanone, 1-phenyl-1-pentanone, 1-phenyl-1-hexanone, 1-phenyl-2-propanone, 2-hydroxyacetophenone, 2'-hydroxyacetophenone, 1-phenylpropane-1,2-dione, benzophenone, (R)-(-)-benzoin, propanol, butanol, rac-2-propanol, 2-methylpropanol, 3-methylbutanol, rac-3-methyl-2-butanol, (S)-4-methyl-2-pentanol, (R)-4-methyl-2-pentanol, (R)-2-hexanol, (R)-2-octanol, rac-1-amino-2-propanol, rac-1-bromo-2-propanol, butan-2,3-diol, rac-decan-1,2-diol, 3-hydroxybutanoic acid, 2-hexyn-1-ol, (R)-1-phenylethanol, 2-phenylethanol, rac-benzoin, meso-hydrobenzoin, (S,S)-hydrobenzoin, and (R,R)-hydrobenzoin. No activity is detected for isoform SDR B3 with isobutyraldehyde, 2-hexanone, 2-heptanone, 2-octanone, ethyl acetoacetate, acetophenone, 1-phenyl-1-butanone, 1-phenyl-1-pentanone, 1-phenyl-1-hexanone, 1-phenyl-2-propanone, 2-hydroxyacetophenone, 2'-hydroxyacetophenone, 4-(4-hydroxyphenyl)-2-butanone, 1-phenylpropane-1,2-dione, benzophenone, rac-benzoin, (R)-benzoin, propanol, butanol, rac-2-propanol, 2-methylpropanol, 3-methylbutanol, (R)-4-methyl-2-pentanol, (R)-2-hexanol, rac-1-amino-2-propanol, rac-1-bromo-2-propanol, butan-2,3-diol, 3-hydroxybutanoic acid, 2-hexyn-1-ol, (R)-1-phenylethanol, 2-phenylethanol, rac-benzoin, meso-hydrobenzoin, (S,S)-hydrobenzoin, and (R,R)-hydrobenzoin Cupriavidus necator H16 / ATCC 23440 / NCIB 10442 / S-10-1 ?
-
 ?
additional information the enzyme accepts both NAD+ and NADP+. However, purified enzyme shows strong preference for NADP+ Cupriavidus necator H16 / ATCC 23440 / NCIB 10442 / S-10-1 ?
-
 ?
rac-4-methyl-2-pentanol + NADPH + H+
-
 Cupriavidus necator ?
-
 ?
rac-butan-2,3-diol + NADPH + H+
-
 Cupriavidus necator ?
-
 ?

Synonyms

Synonyms Comment Organism
SDR A5
-
 Cupriavidus necator
SDR B3
-
 Cupriavidus necator
short-chain dehydrogenase/reductase
-
 Cupriavidus necator

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
40
-
 isoform SDR A5 Cupriavidus necator
50
-
 isoform SDR B3 Cupriavidus necator

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
4.3
-
 (S)-2-octanol isoform SDR B3, at pH 10.0 and 40°C Cupriavidus necator
13.7
-
 (S)-2-octanol isoform SDR A5, at pH 10.0 and 40°C Cupriavidus necator

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6.5
-
 for the reduction reaction Cupriavidus necator
11
-
 for the oxidation reaction Cupriavidus necator

Cofactor

Cofactor Comment Organism Structure
NADP+
-
 Cupriavidus necator
NADPH preferred cofactor Cupriavidus necator

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
0.6
-
 (S)-2-octanol isoform SDR B3, at pH 10.0 and 40°C Cupriavidus necator
1.8
-
 (S)-2-octanol isoform SDR A5, at pH 10.0 and 40°C Cupriavidus necator