Cloned (Comment) | Organism |
---|---|
gene xyl2, the xylose-metabolic gene XYL2 from Scheffersomyces stipitis is introduced into Saccharomyces cerevisiae | Scheffersomyces stipitis |
Protein Variants | Comment | Organism |
---|---|---|
additional information | construction of efficient xylose-fermenting Saccharomyces cerevisiae strain DXS through a synthetic isozyme system of xylose reductase from Scheffersomyces stipitis. The xylose-metabolic genes XYL1 (EC 1.1.1.307), XYL2, and XYL3 (EC 2.7.1.17) from Scheffersomyces stipitis are introduced into Saccharomyces cerevisiae. Construction of control strains SR6 and MM through random integration of the XR (NADPH) or mXR (NADH) expression cassette, respectively, at the multipled elements of the genome of Saccharomyces cerevisiae strain D452-2. Fermentation parameters of engineered Saccharomyces cerevisiae strains in mixed sugar fermentations (glucose and xylose), overview | Scheffersomyces stipitis |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
xylitol + NAD+ | Scheffersomyces stipitis | - |
D-xylulose + NADH + H+ | - |
r | |
xylitol + NAD+ | Scheffersomyces stipitis NRRL Y-11545 | - |
D-xylulose + NADH + H+ | - |
r | |
xylitol + NAD+ | Scheffersomyces stipitis NBRC 10063 | - |
D-xylulose + NADH + H+ | - |
r | |
xylitol + NAD+ | Scheffersomyces stipitis ATCC 58785 | - |
D-xylulose + NADH + H+ | - |
r |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Scheffersomyces stipitis | P22144 | - |
- |
Scheffersomyces stipitis ATCC 58785 | P22144 | - |
- |
Scheffersomyces stipitis NBRC 10063 | P22144 | - |
- |
Scheffersomyces stipitis NRRL Y-11545 | P22144 | - |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
xylitol + NAD+ | - |
Scheffersomyces stipitis | D-xylulose + NADH + H+ | - |
r | |
xylitol + NAD+ | - |
Scheffersomyces stipitis NRRL Y-11545 | D-xylulose + NADH + H+ | - |
r | |
xylitol + NAD+ | - |
Scheffersomyces stipitis NBRC 10063 | D-xylulose + NADH + H+ | - |
r | |
xylitol + NAD+ | - |
Scheffersomyces stipitis ATCC 58785 | D-xylulose + NADH + H+ | - |
r |
Synonyms | Comment | Organism |
---|---|---|
XDH | - |
Scheffersomyces stipitis |
XYL2 | - |
Scheffersomyces stipitis |
xylitol dehydrogenase | - |
Scheffersomyces stipitis |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NAD+ | - |
Scheffersomyces stipitis | |
NADH | - |
Scheffersomyces stipitis |
General Information | Comment | Organism |
---|---|---|
metabolism | one xylose-assimilating pathway consists of xylose reductase (XR, XYL1) and xylitol dehydrogenase (XDH, XYL2, EC 1.1.1.9) from Scheffersomyces stipitis. XR reduces xylose to xylitol by using NAD(P)H as cofactor and XDH further oxidizes xylitol to xylulose using NAD+. While the XR-XDH pathway can offer higher metabolic fluxes than the xylose isomerase (XI) pathway, it accumulates xylitol which is produced due to cofactor imbalance caused by different cofactor requirement between XR and XDH | Scheffersomyces stipitis |