Cloned (Comment) | Organism |
---|---|
expressed in Saccharomyces cerevisiae strain DX23 | Scheffersomyces stipitis |
recombinant overexpression in Saccharomyces cerevisiae strain D452-2, coexpression with Saccharomyces cerevisiae enzymes/genes xylulokinase XKS1, and xylose reductase XYL1 or XYL3, aldose reductase GRE3. Xylose fermentation efficiency of a strain overexpressing GRE3 is dramatically increased by high expression levels of XYL2 | Scheffersomyces stipitis |
Protein Variants | Comment | Organism |
---|---|---|
additional information | engineered Saccharomyces cerevisiae strain DGX23 overexpressing aldose reductase GRE3, xylitol dehydrogenase XYL2, and xylulokinase XYL3 can ferment xylose as well as a mixture of glucose and xylose with higher ethanol yields and productivities than those of an isogenic strain overexpressing xylose reductase XYL1, xylitol dehydrogenase XYL2, and xylulokinase XYL3 under oxygen-limited conditions. Optimized expression levels of GRE3, XYL2, and XYL3 can overcome redox imbalance during xylose fermentation by engineered Saccharomyces cerevisiae under oxygen-limited conditions, anaerobic xylose fermentation | Scheffersomyces stipitis |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
xylitol + NAD+ | Scheffersomyces stipitis | - |
D-xylulose + NADH + H+ | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Scheffersomyces stipitis | P22144 | - |
- |
Scheffersomyces stipitis | P22144 | i.e Pichia stipitis | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
xylitol + NAD+ | - |
Scheffersomyces stipitis | D-xylulose + NADH + H+ | - |
? |
Synonyms | Comment | Organism |
---|---|---|
XYL2 | - |
Scheffersomyces stipitis |
xylitol dehydrogenase | - |
Scheffersomyces stipitis |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NAD+ | - |
Scheffersomyces stipitis | |
NAD+ | specific for | Scheffersomyces stipitis |