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Literature summary for 1.1.1.9 extracted from

  • Poonperm, W.; Takata, G.; Morimoto, K.; Granstroem, T.B.; Izumori, K.
    Production of L-xylulose from xylitol by a newly isolated strain of Bacillus pallidus Y25 and characterization of its relevant enzyme xylitol dehydrogenase (2007), Enzyme Microb. Technol., 40, 1206-1212.
No PubMed abstract available

Inhibitors

Inhibitors Comment Organism Structure
Cu2+
-
Aeribacillus pallidus
Zn2+
-
Aeribacillus pallidus

Localization

Localization Comment Organism GeneOntology No. Textmining
additional information no activity in membrane fraction Aeribacillus pallidus
-
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
28000
-
x * 28000, SDS-PAGE Aeribacillus pallidus

Organism

Organism UniProt Comment Textmining
Aeribacillus pallidus
-
-
-
Aeribacillus pallidus Y25
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
Aeribacillus pallidus

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
23.5
-
-
Aeribacillus pallidus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
D-iditol + NAD+ 11.2% of the activity with xylitol Aeribacillus pallidus ?
-
?
D-iditol + NAD+ 11.2% of the activity with xylitol Aeribacillus pallidus Y25 ?
-
?
D-threitol + NAD+ 109% of the activity with xylitol Aeribacillus pallidus ?
-
?
D-threitol + NAD+ 109% of the activity with xylitol Aeribacillus pallidus Y25 ?
-
?
additional information the enzyme is specific for polyols that have a hydroxyl group at the C-2 and C-3 positions in the L- and D-sides, respectively, in the Fischer projection Aeribacillus pallidus ?
-
?
additional information the enzyme is specific for polyols that have a hydroxyl group at the C-2 and C-3 positions in the L- and D-sides, respectively, in the Fischer projection Aeribacillus pallidus Y25 ?
-
?
xylitol + NAD+
-
Aeribacillus pallidus L-xylulose + NADH + H+
-
?
xylitol + NAD+
-
Aeribacillus pallidus Y25 L-xylulose + NADH + H+
-
?

Subunits

Subunits Comment Organism
? x * 28000, SDS-PAGE Aeribacillus pallidus

Synonyms

Synonyms Comment Organism
NAD-dependent xylitol dehydrogenase
-
Aeribacillus pallidus
XDH-Y25
-
Aeribacillus pallidus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
40
-
-
Aeribacillus pallidus

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
20 50 20°C: about 50% of maximal activity, 50°C: about 50% of maximal activity Aeribacillus pallidus

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
-
40 10 min, 2.5 mM NAD+, stable Aeribacillus pallidus
20
-
at room temperature, the enzyme is inactivated within 2 days Aeribacillus pallidus
50
-
10 min, 2.5 mM NAD+, about 50% loss of activity Aeribacillus pallidus
60
-
10 min, 2.5 mM NAD+, about 80% loss of activity Aeribacillus pallidus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
10
-
-
Aeribacillus pallidus

pH Stability

pH Stability pH Stability Maximum Comment Organism
8 10 4°C, 60 min, stable Aeribacillus pallidus