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Literature summary for 1.1.1.9 extracted from

  • Böer, E.; Wartmann, T.; Schmidt, S.; Bode, R.; Gellissen, G.; Kunze, G.
    Characterization of the AXDH gene and the encoded xylitol dehydrogenase from the dimorphic yeast Arxula adeninivorans (2005), Antonie van Leeuwenhoek, 87, 233-243.
    View publication on PubMed
Search for more literature for 1.1.1.9

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Blastobotrys adeninivorans

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.7
-
 D-ribulose 35°C, pH 7.5, native enzyme Blastobotrys adeninivorans
0.9
-
 L-sorbose 35°C, pH 7.5, native enzyme Blastobotrys adeninivorans
2
-
 D-xylulose 35°C, pH 7.5, native enzyme Blastobotrys adeninivorans
4
-
 xylitol 35°C, pH 7.5, native enzyme Blastobotrys adeninivorans
4
-
 D-sorbitol 35°C, pH 7.5, native enzyme Blastobotrys adeninivorans
5.2
-
 xylitol 35°C, pH 7.5, recombinant enzyme Blastobotrys adeninivorans

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
40300
-
 2 * 40300, calculated from sequence Blastobotrys adeninivorans
72000
-
 native enzyme, gel filtration, non-denaturing PAGE Blastobotrys adeninivorans
80000
-
 recombinant enzyme, gel filtration, non-denaturing PAGE Blastobotrys adeninivorans

Organism

Organism UniProt Comment Textmining
Blastobotrys adeninivorans Q6KAV2
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Blastobotrys adeninivorans

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
D-ribulose + NADH + H+
-
 Blastobotrys adeninivorans ?
-
 ?
D-sorbitol + NAD+
-
 Blastobotrys adeninivorans D-fructose + NADH + H+
-
 ir
L-sorbose + NADH + H+
-
 Blastobotrys adeninivorans ?
-
 ?
ribitol + NAD+
-
 Blastobotrys adeninivorans ?
-
 ?
xylitol + NAD+
-
 Blastobotrys adeninivorans D-xylulose + NADH + H+
-
 r

Subunits

Subunits Comment Organism
dimer 2 * 40300, calculated from sequence Blastobotrys adeninivorans

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
35
-
 native and recombinant enzyme Blastobotrys adeninivorans

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
74
-
 D-ribulose 35°C, pH 7.5, native enzyme Blastobotrys adeninivorans
216
-
 L-sorbose 35°C, pH 7.5, native enzyme Blastobotrys adeninivorans
302.9
-
 D-xylulose 35°C, pH 7.5, native enzyme Blastobotrys adeninivorans
528
-
 D-sorbitol 35°C, pH 7.5, native enzyme Blastobotrys adeninivorans
2115
-
 xylitol 35°C, pH 7.5, recombinant enzyme Blastobotrys adeninivorans
2644
-
 xylitol 35°C, pH 7.5, native enzyme Blastobotrys adeninivorans

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5
-
 native and recombinant enzyme Blastobotrys adeninivorans

pH Stability

pH Stability pH Stability Maximum Comment Organism
7.2 7.9 stable Blastobotrys adeninivorans

Cofactor

Cofactor Comment Organism Structure
NAD+ no activity with NADP+ Blastobotrys adeninivorans
NADH no activity with NADPH Blastobotrys adeninivorans